Báo cáo y học: " Model-driven analysis of experimentally determined growth phenotypes for 465 yeast gene deletion mutants under 16 different conditions" potx

... negatives. 0.85 0.90 0.95 1.00 0.20 0.30 0.40 0.50 0.60 0.70 0.65 0.70 0.75 0.80 0.85 0.90 0.95 0.75 0.80 0.85 0.90 0.95 YPAc YPEtOH YPGal YPD YPGly YPLac YPTE no O2 YPRaff SCade Scall SCarg SCino SClys SCmet SD minimal media YPTE no glucose YPAc YPEtOH YPGal YPD YPGly YPLac YPTE no O2 YPRaff SCade Scall SCarg SCino SClys SCmet SD ... O2 YPRaff SCade Scall SCarg SCino SClys SCmet SD minimal m...

Ngày tải lên: 14/08/2014, 21:20

... for analysis. The intracellular location of the Bag-1 Morpho/AS was confirmed by fluorescent microscopy. Cell viability assay Cell viability assays were performed after 12 and 24 hours of ... used for the PCR were as follows: 94°C for 2 min, 30 cycles of 94°C for 45 sec, 68°C for 45 sec, and 74°C for 1 min, with final extension at 74°C for 3 min. The integrity of...

Ngày tải lên: 31/10/2012, 15:12

... of hCDC4 can also be observed in Acute Myeloid Leukemia (AML) or high risk Myelodysplastic Syndrome (MDS). Therefore we carried out a mutational analysis of the hCDC4 gene in 35 samples of ... Terminator system (Applied Biosystems, Germany). Sequences were analyzed using the Chromas software (Technely- sium Pty Ltd, Tewantin, Australia) and GeneDoc software (http://www.psc.ed...

Ngày tải lên: 31/10/2012, 16:49

... genes identified in the array analysis. Moreover, Western blot analysis of thymo- cyte lysates also confirmed that IgM increases with age (Figure 3). Flow cytometry analysis shows that there is ... and array analysis. For each array sample, the RNA was prepared from the purified thymocytes. The thymocytes were processed by using the RNEasy Mini Kit (Qiagen, Valencia, CA). Quali...

Ngày tải lên: 03/11/2012, 11:35

... as marker. Chemical deglycosylation DM64 was chemically deglycosylated with anhydrous trifluoromethanesulfonic acid (TFMS) using the GlycoFree kit k-500 from Oxford GlycoSystems, USA and submitted to ... potentiated by enzymatic degradation of phospholipids [7,44]. The capacity of DM64 to inhibit the myotoxicity induced by myotoxins I (Asp49) and II (Lys49) from B. asper venom was analyzed...

Ngày tải lên: 21/02/2014, 01:21

... H.S., Han, Y. & Fung, B.K K. (2000) Subunit e xchange of small h eat sho ck proteins. Analysis of olig- omer formation of aA-crystallin and Hsp27 by fluorescence res- onance energy transfer ... disassembly of active units from an oligomeric (storage) state of a-crystallin was proposed, upon structural analysis of aA-crystallin by site- directed spin labelling, as a model...

Ngày tải lên: 22/02/2014, 04:20

... irre- versible formation of gamma glutamyl-lysine bonds [4]. Furthermore, tTG plays a role in the transduction of extracellular signals, mediated by its additional GTP- hydrolyzing activity [5], analogous ... phage carrying a single antibody specificity. We have recently made and selected phage antibody libraries from lymphocytes of CD patients [16] . We were able to isolate single-chai...

Ngày tải lên: 08/03/2014, 09:20

... 2-N-(3-O- acyl 4 -acyl 2 )-b-GlcN-(1–6)-2-N-acyl 1 )3-O-acyl 3 -GlcN where acyl 1 ,acyl 2 and acyl 3 are 3-hydroxyhexadecanoic or 3-hy- droxyoctadecanoic acids, acyl 4 is tetradecanoic or (minor) hexadecanoic ... of Kdo to the lipid moiety [15]. Acyl residues at each acylation position in F. tularensis lipid A can be of two different chain lengths (C14/C16, C16/ C18). Such random distri...

Ngày tải lên: 08/03/2014, 09:20

... core-oligosaccha- rides of E. coli F653 LPS for which only preliminary data were available and investigated the deacylated LPS by NMR and MS. The proposed structure determined pre- viously by methylation analysis ... chemical analysis of 7-O-(2-amino-2-deoxy-a- D -glucopyranosyl- L -glycero- D -manno-heptose as a constituent of the lipopolysaccharides of the UDP-galactose epim...

Ngày tải lên: 08/03/2014, 09:20

... 2002 Kinetic analysis of hydroxylation of saturated fatty acids by recombinant P450foxy produced by an Escherichia coli expression system 7 Tatsuya 7 Kitazume 1 , Akinori Tanaka 1 , Naoki Takaya 1 , ... Sudo, Y. & Beppu, T. (1985) Subterminal hydro- xylation of fatty acids by a cytochrome P-450-dependent enzyme system from a fungus, Fusarium oxysporum. J. Biochem. 97, 755– 763....

Ngày tải lên: 08/03/2014, 22:20