... work, wedescribe the cloning of the gene sequence of BmKIM, the functional expression of the recombinant toxin in Escheri-chia coli and its effect on the sodium channels of neurons and ventricular ... asprimers, and the BmKIM genomic DNA as template forPCR, the nucleotide sequence obtained was the same as the cDNA. Therefore, there is only one intron in the sequence encoding the signal peptide. The ... toxins from BmK (BmKIM, BmKAEP and BmKIT2), but Arg6 or Lys6 and Thr57 are conserved inother scorpion species. Cloning and analysis of genomic sequence of BmKIMWe have isolated, cloned and sequenced...