Báo cáo sinh học: "Comparative analysis of macrophage associated vectors for use in genetic vaccine" ppt

... significantly higher in macro- phage cell line in comparison to non -macrophage cells. The ratio of macrophage/ non -macrophage evaluation was the highest in macrosalin as an indicator of macrophage specificity. ... the effect of cell dominant expression on the immune response and indication of protective ability would be interesting. In addition, targeting macrophage...

Ngày tải lên: 14/08/2014, 19:22

... staining and immunocytochemistry (ICC) SH-SY5Y cells and mixed spinal cord cell cultures were processed for β-Gal staining 3 days after transduction using an X-Gal staining kit (Invitrogen). In ... monitored for limb weakness, paralysis, and infection, as well as for symptoms of neurogenic pain including limb mutilation. Animals were euthanized 4 weeks after injections. β -Gal stain...

Ngày tải lên: 14/08/2014, 19:22

... 29.5-G tuberculin syringe was used to perform single intra-mus- cular (IM) injections of each vector formulated in phos- phate-buffered saline (total volume of 35 μL per injection). The bevel of the ... expressing sarcoglycan led to reduction or elimination of myofiber loss. Mitigating the membrane instability inherent in dystrophic muscle was able to prolong the life of individ...

Ngày tải lên: 14/08/2014, 19:22

... functions of lectins in the plant body. From an evolutionary viewpoint, it is of interest to argue the issue of origin of sugar-binding specificities of JRLs, in the particular context of biological ... conserved in both N-terminal and C-terminal CRDs in CCA and CRLL, all of these CRDs are likely to maintain a sugar-binding function. Thus, dis- tribution of a similar ty...

Ngày tải lên: 30/03/2014, 16:20

... infant foods. In this paper, we report the site-by-site analysis of the maize recombinant lactoferrin (mLf) in comparison with the lactoferrin pro- duced in tobacco (tLf), used as a model for dicotyledons. For ... located in the C-terminal part of the glycoprotein but is mostly unglycosylated [18,19]. Human lactoferrin is also an interesting model because it is a natural def...

Ngày tải lên: 31/03/2014, 07:20

... connector dropping below the chain of matches indicates the amount of DNA missing in BtI1 versus 0305φ8-36. A triangle above the chain of matches indicates the amount of DNA in BtI1 in excess over ... about the modes of evolution affecting the phage. Intragenomic exchange Several patterns of domain reuse cited in the results can be interpreted in terms of a process b...

Ngày tải lên: 18/06/2014, 18:20

... 6B). Discussion Induct ion of therapeutic toleranc e is of increasing inter- est in autoimmuni ty, allograft rejection, allergy, ast hma, and various forms of hypersensitivity. Because of their capacity ... the use of dexamethasone in the induction of tolerogenic DCs. Handb Exp Pharmacol 2009, , 188: 233-49. 46. Fischer R, Turnquist HR, Taner T, Thomson AW: Use of rapamy...

Ngày tải lên: 18/06/2014, 19:20

... linked to three cellular pathways in cluding p53, phosphatidyl inositol 3 (PI3) kinase, and apoptosis. The 14-3-3 proteins are a family of multifunctional phosphoserine/phosphothreonine binding ... forms of cancers [10]. Phosphorylatio n and dephosphorylation of specific amino acid residues, including serine, tyrosine and threonine, by specific kinases and phosphatases is a major form...

Ngày tải lên: 18/06/2014, 19:20

... were removed for spectratyping analysis of TCR repertoire of inf iltrating T cells. Earlier studies suggested this time point to be the best for examining implant infiltrate, before rejection ... is found in B cell reconstituted mice. Lack of disease in controls clearly indicated a key role for B cells in the onset of pathology. Phenotypic analysis of lymphocyte infi...

Ngày tải lên: 18/06/2014, 19:20

... by immunoblot. Mutational analysis of the putative catalytic and zinc-binding residues of G1L utilizing a trans complementation assayFigure 4 Mutational analysis of the putative catalytic and zinc-binding residues of ... one of which is believed to be the essential downstream residue involved in the coordination of a zinc ion (Fig. 2). A library of C-terminally flag-tagged...

Ngày tải lên: 19/06/2014, 08:20