Báo cáo sinh học: "Altering a-dystroglycan receptor affinity of LCMV pseudotyped lentivirus yields unique cell and tissue tropism" docx

... a-dystroglycan receptor affinity of LCMV pseudotyped lentivirus yields unique cell and tissue tropism. Genetic Vaccines and Therapy 2011 9:8. Submit your next manuscript to BioMed Central and take ... and Therapy 2011, 9:8 http://www.gvt-journal.com/content/9/1/8 Page 13 of 13 RESEARCH Open Access Altering a-dystroglycan receptor affinity of LCMV pse...

Ngày tải lên: 14/08/2014, 19:22

... percentage of SGHPL-4 and HFF cells expressing IE 1/2 over a period of time. Representative images of HCMV IE 1/2 are depicted at 8 h p.i in (B) CTBs and (C) HFFs and at 120 h p.i. in (D) CTBs and ... three fields of view were considered and the percent of IE-positive cells was calculated as: (average number of IE-stained cells/average number of DAPI-stained cells) ×...

Ngày tải lên: 18/06/2014, 22:20

... formalin-fixed and paraffin-embedded tumor tissue blocks were retrieved from the files of the Institute of Pathology (University Hospital of Essen, Germany) and processed using the tissue microarray ... pattern. One tissue core of each normal thyr- oid and kidney tissues in preset position in each block served as control tissue and helped with the orientation. 5 μm TMA...

Ngày tải lên: 18/06/2014, 22:20

... variable virus receptor affini- ties [91,92], evolutionary adaptation of receptor affinity [93], emergence of escape variants with altered receptor affinities [94], temporal alteration of receptor ... both non-inflammatory receptor blockade and immune-medi- ated inflammation directed at viral protein -receptor com- plexes could cause pathology of tissues non-permissive...

Ngày tải lên: 19/06/2014, 08:20

... distribution of apop- totic cells or pyknotic cell clusters in the neuroepithelium of Ptdsr -/- and Ptdsr +/+ mice. Thus, reduced cell death or dimin- ished clearance of apoptotic neural progenitor cells ... number of receptors have been iden- tified that can mediate phagocytosis of apoptotic cells. These include, for example, scavenger receptors and pattern recognition rece...

Ngày tải lên: 06/08/2014, 18:21

... methylcellulose culture for PCR analysis of the presence of the pIL3 plasmid. DNA and RNA analyses The simultaneous isolation of total cellular RNA and DNA from tissues or cells was performed using TriPure ... adrenal glands, and brain. PCR analysis of genomic DNA and RNA isolated from bone marrow and blood (or serum) was performed using primer specific pIL3 sequences. Speci...

Ngày tải lên: 14/08/2014, 19:22

... type I receptor specificity profiles of BMP-2, BMP-6, and BMP-7, we used in vitro interaction analy- sis (Table 1). Ligand proteins of BMP-2 (E. coli), BMP-6 (CHO cells) and BMP-7 (NS0 cells) were immobilized ... microgram of BMP-6, with a reac- tion time of 8 h at 37 °C. For glycosylation trimming, a mixture of 1 mU of PNGase F3 and 1 mU of PNGase H was used per microgram...

Ngày tải lên: 23/03/2014, 07:20

... the surface of virions and infected cells [23]. To gain further insight into the structural properties of gp64 and to increase the accessibility of peptide insertions to their ligands, we inserted ... the surface of infected cells was determined by FACS analysis 24 and 48 h.p.i. For this purpose Sf9 cells were infected three times, independently with an m.o.i. of 10 using 6-we...

Ngày tải lên: 23/03/2014, 21:21

... United Kingdom of Great Britain and Northern Ireland, 4 Tameside General Hospital, Ashton-under-Lyne, OL6 9RW, United Kingdom of Great Britain and Northern Ireland and 5 Monklands Hospital, ... 8HE, United Kingdom of Great Britain and Northern Ireland, 2 Harrogate District Hospital, Harrogate, North Yorkshire, HG2 7SX, United Kingdom of Great Britain and Northern Irelan...

Ngày tải lên: 18/06/2014, 17:20

... self-help groups and PLHIV, and by HIV counsellors at the VCT centre of the Impact theory of PEPPFigure 1 Impact theory of PEPP. PEPP was designed to promote community awareness, empowerment and participation ... combination of activities, including participatory trainings for peer educators, outreach educational activities with distribution of IEC materials, and referrals...

Ngày tải lên: 18/06/2014, 17:20