Báo cáo sinh học: "Development of avian influenza virus H5 DNA vaccine and MDP-1 gene of Mycobacterium bovis as genetic adjuvant" pptx

... Development of avian influenza virus H5 DNA vaccine and MDP-1 gene of Mycobacterium bovis as genetic adjuvant Genetic Vaccines and Therapy 2010, 8:4 Jalilian et al. Genetic Vaccines and Therapy ... original work is properly cited. Research Development of avian influenza virus H5 DNA vaccine and MDP-1 gene of Mycobacterium bovis...

Ngày tải lên: 14/08/2014, 19:22

... there was an average reduction of 99.1% of infectious virus particles (Fig. 2). At an MOI of 5 there was an aver- age reduction of 95.7%, and at an MOI of 10 there was an average reduction of 90.3% ... In the presence of TET, the recombinant virus grew to the same yield and with the same kinetics as wild type virus while in the absence of TET the production of in...

Ngày tải lên: 18/06/2014, 22:20

... the idea of the handbook and managed the project. HN conducted a detailed review of the literature and produced the first drafts of the handbook and this paper. SP provided case studies and technical ... issues of academic honesty and correct referencing of material used in assignments. This would include clearly defined penalties for plagiarism and collu- sion. 4. Quali...

Ngày tải lên: 18/06/2014, 17:20

... was on assumption of 100% RT efficiency. This method allows a calculation of the minimum amount of NoV present based on cDNA values. Ideally quantification of an RNA virus involves the use of ... purposes) Start DNA Master SYBR Green I (Roche). A final reaction volume of 20 µl containing 0.5 µl of cDNA, 2 µl of SYBR green Mastermix, 2.8 µl of 25 mM MgCl 2 (3.5 mM), 1 µl...

Ngày tải lên: 18/06/2014, 18:20

... 2C–D). As is seen in all other models of filovirus infection, indicators of liver health such as alanine transaminase (ALT) and aspartate transaminase (AST) function increased as the MARV dis- ease ... the spleen and liver, and an equivalent mean time to death. Existence of the EBOV mouse model has increased our understanding of host responses to filovirus infections and...

Ngày tải lên: 18/06/2014, 18:20

... ET PCO2 and SpO 2 [14]. IntelliVent™ has been assessed in one clinical trial on feasibility and safety in children during the weaning phase. Fifteen children were included and IntelliVent™ was ... adjustments of PEEP in the case of hemodynamic instability based on the presence of pulsus paradoxus on pulse oximetry waveform (heart lung index) [37, 38]. The accuracy of the w...

Ngày tải lên: 18/06/2014, 18:20

... a region of the polymerase gene of PcyRV2 that could be compared to the sequence available for PapRV2. DNA sequence for 352 bp of the DNA polymerase gene was obtained. An alignment of this sequence ... Primer Standard curves obtained from the RV2 rhadinovirus and OSM reference cellular gene assaysFigure 4 Standard curves obtained from the RV2 rhadinovirus and OSM referen...

Ngày tải lên: 18/06/2014, 22:20

... infecting virus and one uninfected well for each virus) was processed for staining and the supernatant from each chamber was carefully aspirated and stored at - 80°C. Each chamber was then washed ... remarkably high level of similarity between their env and nef genes as well as their 3' LTR. As this genotype was found in the brains of two separate animals, and it e...

Ngày tải lên: 19/06/2014, 08:20

... ubiq- uitin-degrading enzyme in yeast, as well as the identity of a catalytic triad composed of histidine, cysteine, and aspartic acid, I7L has been classified as a cysteine protein- ase. The thiol reagents ... identification of the catalytic residues and the predicted structure of the I7L proteinase, a new class of small molecule inhibitors was developed that are capa- bl...

Ngày tải lên: 19/06/2014, 08:20

... technol- ogy of computerized image scanning and analysis. It has the advantage of providing more efficient and objective results while automating the most laborious and subjec- tive aspect of the assay ... Fleming DM: Contribution of influenza and respiratory syncytial virus to community cases of influenza- like illness: an observational study. Lancet 2001, 358:1410-141...

Ngày tải lên: 19/06/2014, 08:20