Báo cáo sinh học: "Characterization of a potent non-cytotoxic shRNA directed to the HIV-1 co-receptor CCR5" docx

... following primer pairs: for the antisense strand of CCR5 siRNA (1005); 5'-CTCGAGTCTAGAGAATTCCCCCAGTGGAAA- GAC-3' and 5'-GAATTCCTCGAGGCTAGCAAAAAGAGCA AGCTCAGTTTACACCGGTGTTTCGTCCTTTCCAC-3', ... 5'-GAATTCCTCGAGGCTAGCAAAAAGAGCA AGCTCAGTTTACACCGGTGTTTCGTCCTTTCCAC-3', for the sense strand of CCR5 siRNA (1005); 5'-CTC- GAGTCTAGAGAATTCCCCCAGTGGAAAGAC-3'...

Ngày tải lên: 14/08/2014, 19:22

... structures of T. versicolor [30,31], and Melanocarpus albomyces [32] laccases are compared (not shown). As folding of the backbone around the metal center was already proposed to be a major factor affecting ... variations found in laccases to the nature of the specific amino acids present in their active site as their oxidative capabilities appear tightly linked to this p...

Ngày tải lên: 08/03/2014, 09:20

... sample cuvette. A stock solution of diethyl pyrocarbonate prepared as above in alcohol was added to the sample cuvette at a final concentration of 0.3 mm. An equal amount of alcohol was added to the reference ... twofold for palmitoleoyl-CoA and cis-vaccenoyl-CoA at lower temperature, these remained largely unaltered for palmi- toyl-CoA, which should result in a higher rati...

Ngày tải lên: 16/03/2014, 13:20

... with anti-Flag serum. The 30 kDa protein band of Flag-tagged DmPCNA2 and the 36 kDa pro- tein band of Flag-tagged DmPCNA1 are indicated by arrows. The sizes of the molecular mass markers are indicated ... transla- tion initiation, 5¢-(C ⁄ A) AA (A ⁄ C)ATG, and a putative poly (A) addition signal sequence, 5¢-AATAAA [17,18]. It encoded a predicted product of 255 amino aci...

Ngày tải lên: 23/03/2014, 10:20

... from the antisense strand. OSM-Mn CCTCGGGCTCAGGAACAAC GTC TACTGCATGGCCCAGCTGCTGGACAA CTCAGACATGA CTGAGCCCACGAAGGCC OSM-AGM A OSM-Human A C.G T OSMa Primer OSM-FAM Probe OSMb Primer Standard ... Primers RDELa 4 ORF 60 bias 5 KSHV 5'-CTTGCCAACGATTACATTTCCAGRGAYGARCT-3' SRDEa 4 ORF 60 bias RRV 5' CTGGCTAACGACTACATCTCCAGRGAYGARCT-3' NFFEa ORF 60 bias KSHV 5'-GGC...

Ngày tải lên: 18/06/2014, 22:20

... mutagenesis at position 5979, upstream the env initiation codon, using the primer 5'-TGCAAATAAAT- GG ATCCAACAAGTAGCAAAAGT-3' (nt 5968 to 6000). Mutagenic primers 5'-GGGACAGCAAGC TAAGTATCAA- 3' ... Disruption of the SD 6123 site (G to C substitution at nt 6124) was achieved with primer 5'-TCTAAAGGATC- CCCCAGCAAGC TAAGTATCAACCCCAG-3' (non CAEV sequence...

Ngày tải lên: 13/08/2014, 06:20

... EB participated in the plan- ning of the experiments, review and interpretation of data and critical review of the manuscript. All authors read and approved the content of the manuscript. Acknowledgements We ... infected, and the spread of the infection to adjacent cells was all but eliminated. A half-maximal inhibitory effect was estimated to occur at 4.4 ± 1.0...

Ngày tải lên: 19/06/2014, 08:20

... 5¢-GACACCGTAGGTTGAGCCGCCAATC GTCCC-3¢; NP5, 5¢-CTTTAACTTGTTGGGCACTGG CATTG-3¢; NP6, 5¢-TTGATCGATTCTGTCTATGCCC CA-3¢ along with the adaptor primers: AP1 (5¢-GTAATAC GACTCACTATAGGGC-3¢) and AP2 (5¢-ACTATAGGG CACGCGTGGT-3¢). Nested ... from these strains (unpublished data). One of the marine bacteria showing peptidase activ- ity was closely related to Serratia proteamaculans of the S...

Ngày tải lên: 19/02/2014, 07:20

... 5¢-ATGTTAAA GAAAGTATTAAGTTGTTG-TATTGCAGC-3¢ and 5¢-AAAGTTTGCTTGGAGCGTCAAGCC-ACTGTAAG CCG-3¢, cloned into the expression vector pBAD-TOPO, transformed into the E.coliTop10 strain and grown on LB agar ... cloning of the proteinase gene primers were designed from the sequence of Vibrio alginolyticus [42]: 5¢-GCG GAATTCTACACCCGCTACATGTGGCGTCG CCAT-3¢ and 5¢-CGC GGATCCTGGGGACTAGATC GAA...

Ngày tải lên: 21/02/2014, 01:21

... melanogaster ejaculatory bulb [30], Mamestra brassicae proboscis [17], labial palps of the moth Cactoblastis cactorum [14] and cells underlying the cuticle in Phasmatodea and Orthoptera [31]. Although ... native signal peptide was amplified by PCR using the following primers: 5¢ primer, 5¢-GAGCCCGGATCCACCATGAA GGTCTCAATAATT 3¢;3¢ primer, 5¢-CTGACG GAAT TCTTAAACATTAATGCC 3¢. These prime...

Ngày tải lên: 21/02/2014, 03:20