Báo cáo sinh học: "DNA vaccine constructs against enterovirus 71 elicit immune response in mice" ppt

... include formalin-inactivated whole virus vac- cine, DNA vaccine and recombinant protein vaccine. These vaccine constructs remain promising vaccine strate- gies that require further refinement, thus ... biotinylated lysine residues were incorporated into the VP1 protein during translation. Using the chemiluminescent detection method with streptavidin-alkaline phosphatase in Western...

Ngày tải lên: 14/08/2014, 19:22

... to resource, guide and support training institutions to not only upgrade training but also ensure appropriate initial and continuing education (integrated, multidisciplinary, community- and outcomes-based). Crucially, ... cannot be done in favour of creativ- ity and problem-solving. Competing interests The authors declare that they have no competing interests. Authors' contributions Al...

Ngày tải lên: 18/06/2014, 17:20

... following formulation: [fluorescence intensity in stimulating cells - fluorescence intensity in non-stimulating cells]/fluorescence intensity in stimulating cells X 100. Experimental design In order ... experi- ment was terminated. The lung tissues were embedded in paraffin wax, stained with hematoxylin and eosin, and examined under a light microscope. The lung injury was scored acc...

Ngày tải lên: 18/06/2014, 19:20

... 6955-6965. 11. Hsieh MK, Wu CC, Lin TL. Priming with DNA vaccine and boosting with killed vaccine conferring protection of chickens against infectious bursal disease. Vaccine 2007, 25, 5417-5427. 12. ... DNA vaccine against infectious bursal disease virus expressing VP2 protein and chicken IL-2. Vaccine 2009, 27, 864-869. 18. Larsen DL, Karasin A, Olsen CW. Immunization of...

Ngày tải lên: 07/08/2014, 23:22

... greatest challenge in designing DNA vaccines against parasites is making the vaccine suitable for humans while providing strong, long lasting immune responses. Many studies in laboratory animals ... priming with DNA vaccine for P. falciparum CSP and boosting with a recombinant protein vaccine in adjuvant (RTS, S/AS02A) induced the production of significant antibody and T cell...

Ngày tải lên: 14/08/2014, 19:22

... to vaccination against HIV, we ana- lyzed immune responses to an HIV vaccine in mice using adenoviral vectors expressing HIV Env and Gag-Pol alone or in combination with vectors encoding IFNa2, IFNa4, ... vaccine remains elusive. Up to now, innumerable vaccine candi dates have been developed and evaluated in preclinical models, but only three vaccines have been advanced into e...

Ngày tải lên: 13/08/2014, 01:21

... 3044-3048 Garfinkel LI, Periasamy M, Nadal-Ginard B (1982) Cloning and characterization of cDNA sequences corresponding to myosin light chain 1, 2 and 3, troponin-C, troponin-T, ... 60 chromosomal species in the Israeli range persisted during 70 000 years in a continuous range connecting the coastal plains of the Negev and Sinai deserts with n...

Ngày tải lên: 14/08/2014, 19:22

... of administration of DNA vaccines have been studied, including intramuscular, intradermal, subcutaneous, intravenous, intraperitoneal, oral, vaginal, intranasal and, more recently, non-invasive delivery ... against influenza in chickens. [ 14] Influenza virus H5 hemagglutinin (HA) CMV, β-actin, RSV, SV40 Similar in vitro expression of HA. The greatest HA-specific antibody and protecti...

Ngày tải lên: 14/08/2014, 19:22

... autoimmune dis- eases [7,8]. In this context, there is a great interest in the development of new vaccines against TB. A number of alternative living and non-living putative TB vaccines are being ... studies, is that BCG vaccine protects against dissem- inated disease in newborns and children. In addition, this immunity wanes with age, resulting in insufficient protec- tion a...

Ngày tải lên: 14/08/2014, 19:22

... tattoo elicited much higher specific humoral immune responses than three or even four intra- muscular injections. Further, tattooing induced higher specific cellular immune responses than intramuscular DNA ... the tattoo delivery induced more robust immune responses than intramuscular deliv- ery that was in concordance with previous findings of Bins and coworkers [6]. However, in ou...

Ngày tải lên: 14/08/2014, 19:22