Báo cáo sinh học: "Electroporation by nucleofector is the best nonviral transfection technique in human endothelial and smooth muscle cells" pdf

... Access Methodology Electroporation by nucleofector is the best nonviral transfection technique in human endothelial and smooth muscle cells Nina Iversen*, Baard Birkenes, Kari Torsdalen and Srdjan Djurovic Address: ... participated in the design and coordination of the study, established the transfection methods electropora- tion, nucleofection and...

Ngày tải lên: 14/08/2014, 19:22

... esponse by the probe (i.e. an increase in the emission ratio from base- line) and (2) the rate of this response. Analyses involved comparing responses to isoproterenol and a range of clinically ... states resulting in the favouring of one downstream pathway over another which has intriguing therapeutic implications. Despite the recent expansion of studies investigating lig...

Ngày tải lên: 12/08/2014, 13:22

... variance explained by this QTL. This weighted mean information content is presented in Table III for candidates of years 2004 and 2006 and for their sires and dams. For all the traits, information content ... Tab. I). Therefore, the MAS evaluation is more demanding in computational terms but the information on QTL is increasing with time. More families are genotyped a...

Ngày tải lên: 14/08/2014, 13:22

... active site of the protease [8]. Assembly of the complex is initiated in the ER, where Aph-1 and nicastrin interact, followed by binding of PS. Thereafter, Pen-2 binds to the com- plex and facilitates ... better in SH-SY5Y cells than in brain samples. Difficulties in obtaining pure DRM fractions from brain tissue are probably due to the heterogeneity of the sample...

Ngày tải lên: 30/03/2014, 04:20

... important clinico- pathological role in the lung cancer. It will be of consid- erable interest to further understand these interactions and elucidate the intrinsic mechanism s, since one of the most ... analysis of LCMR1 in normal human tissues. ZL prepared GST- LCMR1 protein and antibody. CL participated in the qPCR and drafted the manuscript. ZL and XM performed im...

Ngày tải lên: 10/08/2014, 10:21

... δ) is a non-increasing function in δ. We are interested in determining the numbers δ k (n)where δ k (n)=max{δ |γ(n, δ) ≥ k},k≥ 1. Since the domination number of an (n, δ)-graph G is 1 if and ... +1≥ √ 2n −1. Since this is larger that the previous bound, we obtain the desired lower bound for |T |. The following theorem is immediate from Lemmas 3 and 5. Theorem 8 If T...

Ngày tải lên: 07/08/2014, 06:22

... in the pathogenesis of disease and aging [7,8]. Normal mitochondrial function is a critical place in maintaining cellular homeostasis because mitochondria produce ATP and they are the major intracellular ... is char- acterised by a decrease in lean body mass and an increase in adipose mass, osteopenia, muscle atrophy, reduced exercise tolerance and changes in th...

Ngày tải lên: 18/06/2014, 22:20

... operator and six PLWHAs – two involved in counselling, two in keeping files and guiding patients to the doctors, and two in organizing home visits of irregular and defaulter patients. In August ... reduction in length of consultations. The most impor- tant gains were in the pre-ART and in ART initiation phases, with lesser gains in the ART follow-up phases....

Ngày tải lên: 18/06/2014, 17:20

... JZ and HL performed the statistical analysis and drafted the manuscript. DW and LL helped in drafting the manuscript and contributed specific information and critical analysis throughout the ... 100%), according to the percen- tages of the posit ive staining areas relative to the entire carcinoma-involved area or, for the normal samples, the entire section. The...

Ngày tải lên: 18/06/2014, 19:20

... interpreted the data and harvested the samples. KA established the immunohistochemistry, analyzed the tissue samples, interpreted the data, and performed the histopatholgic analysis of BRONJ- related and ... mucoperiosteal tissue in BRONJ tissues might be explained by the increased Galectin-3, which is known to mediate inhi- bition of intraoral inflammation [1,16]. G...

Ngày tải lên: 18/06/2014, 19:20