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Báo cáo sinh học: " Effects of recombinant adenovirus-mediated expression of IL-2 and IL-12 in human B lymphoma cells on co-cultured PBMC" pot

Báo cáo sinh học:

Báo cáo sinh học: " Effects of recombinant adenovirus-mediated expression of IL-2 and IL-12 in human B lymphoma cells on co-cultured PBMC" pot

... Combination treatment of< /b> recombinant < /b> IL-2 < /b> and < /b> IL-12 < /b> has been reported to be synergistic for inducing anti-tumor responses in < /b> solid tumors but the potential of < /b> IL-2/< /b> IL-12 < /b> gene modified B cell lymphoma ... NK cells and < /b> activated T cells. Although the combination of < /b> recombinant < /b> IL-2 < /b> and < /b> IL-12< /b> treatment has been reported to be synergistic for inducinganti-tumor responses, systemic administration of < /b> ... IL-2 < /b> and < /b> IL-12< /b> genes by adenoviral vectors, and < /b> monitored cytokine pro-duction and < /b> effects < /b> on proliferation and < /b> cytolytic activity of < /b> co-cultured human < /b> peripheral blood mononuclear cells (PBMC).MethodsCell...
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Báo cáo sinh học: " Yeast expressed recombinant Hemagglutinin protein of Novel H1N1 elicits neutralising antibodies in rabbits and mice" pot

... H1N1HA recombinant < /b> protein To evaluate the elicitation of < /b> neutralising antibodies against the yeast expressed Hemagglutinin recombinant < /b> protein, BALB/c mice and < /b> rabbits were immunised intramuscularly ... target of < /b> neutralizing antibodies that inhibit virus binding to target cells and < /b> are classically detected by the hemagglutination inhibition (HI) assay [6,7,8]. Hence recombinant < /b> HA protein based ... 4mammalian cells, expression < /b> of < /b> recombinant < /b> proteins in < /b> yeast could present a viable alternative in < /b> terms of < /b> large scale vaccine production and < /b> a short time line suitable for rapid response in < /b> influenza...
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Báo cáo sinh học: " Complement component C5a Promotes Expression of IL-22 and IL-17 from Human T cells and its Implication in Age-related Macular Degeneratio" potx

... direct interaction between B7 .1 /B7 .2 on monocytes and < /b> CD28 on T cells, or indirect effects < /b> such as the pro-duc tion of < /b> cytokines. C5a treatment promoted both B7 .1 and < /b> B7 .2 expression < /b> on monocytes ... that C5a may be one of < /b> the factors contributing to the elevatedserum IL-22< /b> and < /b> IL-17 levels in < /b> AMD patients. The possible involvement of < /b> IL-22< /b> and < /b> IL-17 in < /b> the inflammation thatcontributes to AMD ... alone. Interest-ingly, C5a induced expression < /b> of < /b> both cytokines only in< /b> co-cultures of < /b> CD4+T cells and < /b> monocytes, suggestingthat monocytes are necessary for C5a to promote IL-22< /b> and < /b> IL-17 expression.< /b> ...
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Báo cáo sinh học:

Báo cáo sinh học: "Mechanism-related circulating proteins as biomarkers for clinical outcome in patients with unresectable hepatocellular carcinoma receiving sunitinib" potx

... pro-tein (HBx) is expressed in < /b> HBV-infected cells and< /b> enhances VEGF-A expression < /b> by stabilizing the tran-scription factor HIF-1a through inhibition of < /b> HIF-1abinding to VHL [10]. These and < /b> other ... correlation betweenhigh plasma concentrations of < /b> VEGF-C at baseline and< /b> improved clinical outcome, as determined by objectiveresponse (RECIST), TTP, and < /b> OS, with baseline VEGF-C remaining an independent ... . in < /b> a phase II study of < /b> sunitinib in < /b> HCC [13], and < /b> suggest that inhibition of < /b> KIT signal-ing may contribute to sunitinib antitumor activity. Thelack of < /b> early separation in < /b> the sKIT TTP and...
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Báo cáo sinh học:

Báo cáo sinh học: " Simian immunodeficiency virus (SIV) envelope quasispecies transmission and evolution in infant rhesus macaques after oral challenge with uncloned SIVmac251: increased" pdf

... ahomoduplex band that migrated slightly slower than thehomoduplex band characterizing infection pattern B. Onenewborn in < /b> each vaccine group was infected with a SIVvariant of < /b> transmission pattern B. Infection ... Leandro, CA). All gel images were colorreversed to enhance visualization of < /b> banding patterns(e.g. black bands on white background). The number of< /b> heteroduplex bands observed is a measure of < /b> ... corresponded to one of< /b> each of < /b> the two VSEV in < /b> dilution series B and < /b> F. Thus, thedilution of < /b> the virus stock to an RT-PCR endpoint resulted in < /b> 4 independent variants (represented by homoduplexbands)...
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Báo cáo sinh học:

Báo cáo sinh học: "The Drosophila Forkhead transcription factor FOXO mediates the reduction in cell number associated with reduced insulin signaling" potx

... impaired insulin sig-naling. The pathway controlling body size in < /b> response toinsulin therefore bifurcates at the level of < /b> dPKB: dPKB con-trols cell number by inhibiting dFOXO function and < /b> dPKBcontrols ... to changing environmental conditions.Evolutionary conservation of < /b> insulin signaling and< /b> FOXO functionGenetic dissection of < /b> signaling by insulin and < /b> its targetDAF-16 has been pioneered in < /b> C. ... strikingconservation of < /b> insulin receptor function is also reflected in< /b> the conservation of < /b> the intracellular signaling cascade.Binding of < /b> insulin-like peptides to their receptor tyrosinekinases...
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Báo cáo sinh học: "Electroporation by nucleofector is the best nonviral transfection technique in human endothelial and smooth muscle cells" pdf

... (lipofec-tion) for gene transfer into somatic cells has become apopular method of < /b> delivering genes. Interaction betweencationic lipids and < /b> DNA through ionic interaction leads toforming cationic ... competinginterests.Authors' contributionsN.I. participated in < /b> the design and < /b> coordination of < /b> thestudy, established the transfection methods electropora-tion, nucleofection and < /b> PCI, and < /b> ... followingoptimalization of < /b> these methods. She was responsible forwriting the manuscript. B. B. and < /b> K.T. were responsible forperforming experiments independently, and < /b> interpreta-tion of < /b> the...
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Báo cáo Y học: Group IID heparin-binding secretory phospholipase A2 is expressed in human colon carcinoma cells and human mast cells and up-regulated in mouse inflammatory tissues doc

Báo cáo Y học: Group IID heparin-binding secretory phospholipase A2 is expressed in human colon carcinoma cells and human mast cells and up-regulated in mouse inflammatory tissues doc

... sPLA2-IID in < /b> several tissues of < /b> micebefore and < /b> 24 h after injection of < /b> LPS was examined byFig. 4. Expression < /b> of < /b> sPLA2-IID and < /b> other PGE2-biosynthetic enzymes in < /b> human < /b> colon carcinoma cell lines. ... regions [21,22]. Since the multiple cationicresidues in < /b> the corresponding C-terminal portions of < /b> mouse and < /b> human < /b> sPLA2-IIAs and < /b> rat and < /b> human < /b> sPLA2-Vs serveas functional heparin-binding ... respectively. (B) Expression < /b> of < /b> the wild-type (WT) and < /b> two mutants of < /b> mIID in< /b> HEK293 cells, as assessed by RNA blotting. (C) Membrane binding of< /b> the WT and < /b> two mutants of < /b> mIID. After collecting the...
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Báo cáo sinh học: " Effects of transplantation with bone marrowderived mesenchymal stem cells modified by Survivin on experimental stroke in rats" potx

... fluores-cence-activated cell sorting (FACS) as described pre-viou sly [10]. A fter blocking for nonspecific binding withbuffer containing 1% bovine serum albumin, the cells were incubated for 20 minutes at 4°C ... bone marrow. Bone marrow cells were intro-duced into 100-mm dishes and < /b> cultured in < /b> completemedium, consisting of < /b> Dulbecco’s Modified Eagle’s Med-ium (DMEM; Sigma) containing 10% fetal bovine ... in < /b> infectedMSCs supernatants under hypoxic conditions by ELSIA. In < /b> vivo, experiments consisted of < /b> three groups, onereceiving intravenous injection of < /b> 500 μl of < /b> phosphate-buffered saline (PBS)...
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Báo cáo sinh học: " Effects of pedigree errors on the efficiency of conservation decisions" pot

... all contributionswere non-negative. Next, these continuous contributionsper candidate were converted into a desired number of< /b> offspring per candidate. Each generation, mating beganwith a randomly ... proportion of < /b> kinship saved by applying optimal contributions based on observed pedigrees instead of < /b> true pedigrees.ρ is correlation between observed kinship and < /b> true kinship.β1 is regression ... Optimisation of < /b> contribution of< /b> candidate parents to maximise genetic gain and < /b> restrictinginbreeding using semidefinite programming (Open Accesspublication). Genetics Selection Evolution 2007,...
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