Báo cáo sinh học: "Comparison of bovine leukemia virus (BLV) and CMV promoter-driven reporter gene expression in BLV-infected and non-infected cells" ppt

... 75:1095-1103. 30. Xiao J, Buehring GC: In vivo protein binding and functional analysis of cis-acting elements in the U3 region of the bovine leukemia virus long terminal repeat. Journal of Virology 1998, 72:5994-6003. 31. ... mutant form of the tax protein of bovine leukemia virus (BLV), with enhanced transactivation activity, increases expression and propag...

Ngày tải lên: 14/08/2014, 19:22

... contributions All authors were involved in the design of the trial and took part in the interpretation of the data and in revising the manuscript. ES carried out the analysis of data and drafted the manuscript. ... changes in gene expression after i.v. injection of 13, 130, or 260 kBq. The total number of transcripts regulated in the tissues investigated is given...

Ngày tải lên: 20/06/2014, 21:20

... exists as quasispecies (QS) in infected individuals, consisting of a predominant viral variant and related, yet genetically distinct minor variants [1]. The study of HCV QS has historically focused ... allow determination of HCV QS heterogeneity without the need for sequencing (reviewed in [22]). HMA was originally described for analyzing the sequence heterogeneity of the envelo...

Ngày tải lên: 19/06/2014, 08:20

... HAU of JCV. DNA was extracted using a QIAprep Miniprep Spin Kit and eluted in 50 µl of the elution buffer. Two µl of the 1:10 diluted template DNA was used for PCR in a final volume of 20 µl of ... of the above suspension con- taining known HAU of JCV using the QIAprep ® Spin Min- iprep kit (Cat No. 27106), according to the manufacturer's instructions and DNA was e...

Ngày tải lên: 19/06/2014, 08:20

... handling a large number of genetic markers is the random forests methodology [32], which uses ensembles of trees. Each tree is built on a bootstrap sample of the original training data. Within ... than avoiding loss of information resulting from discarding some sire means. Perhaps including all sire samples brings noise, leading to a poorer predictive ability of the selected SNPs....

Ngày tải lên: 14/08/2014, 13:21

... may increase veterinarian and patient acceptance of vaccination by reducing the number of injections and avoiding the use of boosters containing live vectors. Using this technology, vaccinologists ... as a single subcutaneous injection of about 10 5 live bacteria in 50 μl saline. Animals received a single-dose of an intra- muscular injection of 2.5 mg of microspheres in...

Ngày tải lên: 14/08/2014, 19:22

... time point. Duration and levels of skin luciferase expression after deliv-ery of plasmid by EP on days 0, 10, and 20Figure 3 Duration and levels of skin luciferase expression after delivery of plasmid ... levels of skin luciferase expression after deliv-ery of plasmid by EP on days 0, 2, and 4Figure 2 Duration and levels of skin luciferase expression after de...

Ngày tải lên: 14/08/2014, 19:22

... detection of major genes in animal breeding data. Theor. Appl. Genet. 76, 311-319 M6nissier F. (1982) Present state of knowledge about the genetic determination of muscular ... double-muscled trait in cattle. In: Muscle hypertrophy of genetic origin and its use to improve beef production (King J.W.B. & M6nissier F. eds), Martinus Nijho...

Ngày tải lên: 14/08/2014, 20:20

... brain and spleen following an intravenous clone 13 infectionFigure 1 Distribution of LCMV in the brain and spleen following an intravenous clone 13 infection. Representative sagittal brain and ... points post-infection to reveal the distribution of LCMV (green) following an intravenous infection with 2 × 10 6 PFU of clone 13. Note the minimal amount of virus in the brai...

Ngày tải lên: 18/06/2014, 18:20

... glycoprotein gp120 of HIV-1 and HIV-2 and syncytium inhibition with lectins. AIDS 1989, 3:635-41. 32. Malvoisin E, Wild F: The role of N-glycosylation in cell fusion induced by a vaccine recombinant virus ... compounds known to interfere with mannose residues of envelope glycoprotein on HIV infectivity and HIV and measles virus- induced cell fusion [31,32]. The inhibitory a...

Ngày tải lên: 18/06/2014, 22:20