Báo cáo sinh học: "Long term physiologic modification using rAAV in utero gene-therapy" doc

... citation purposes) Effect of in utero rAAVcntf-gfp on water intake in ratsFigure 2 Effect of in utero rAAVcntf-gfp on water intake in rats. Rats were treated in utero with an rAAVcntf-gfp at 16 days ... method of in utero gene transfer with rAAV can be useful in treating metabolic diseases by providing long -term gene expres- sion. A number of experiments involving i...

Ngày tải lên: 14/08/2014, 19:22

... inverse probability of cen- soring weights to adjust estimates of mortality for loss to follow-up using measured covariates [21]. CD4 gain was assessed using two linear generalized esti- mating ... suppression was defined as achieving viral load below 400 copies per ml. Initial viro- logic success was defined as achieving a viral suppression within six months of HAART initiation in pati...

Ngày tải lên: 20/06/2014, 08:20

... management option in CCTGA [24]. In addi- tion, the incidence of heart block was reported to be higher in the anatomic repair group resulting in more pacemaker implants [17,22,24]. In case of severe ... patients were suffering from right-ventricular dysfunction, in 12 of them (six in the non-Rastelli group, six in the Rastelli grou p) progressively, resulting in 10 patients...

Ngày tải lên: 10/08/2014, 09:22

... avoided, since in the cases not diagnosed early, the diaphragm becomes very thin, causing ruptures at the suture line and preventing the tightening of the dia- phragm. Pleural space was drained using ... thoracotomy in the 6th or 7th intercostal space using controlateral single lung ventilation. The hemidiaph- ragm transsected approximately 5 cm initally to avoid intraabdominal organ...

Ngày tải lên: 10/08/2014, 09:22

... proteins is a sobering issue for the ‘post-genomic era’. And finding functionally relevant informa- tion within the non-coding sequence presents a formidable challenge, akin to tracking footprints ... collectively termed the JASPAR database, was produced by the pure determination of Albin Sandelin for his thesis project studying the binding similarities of transcrip- tion factors in the sam...

Ngày tải lên: 06/08/2014, 18:20

... are linked by seven SGI interactions to the bar-1 query gene, 11 fine genetic interactions, 36 co-phenotype links, three coexpression links, and one protein-protein interaction link. To further investigate ... experiments in at least three independent replicates in a blind fashion. Finally, we used a global analysis of our data to identify interacting gene pairs in an unbiased fashion....

Ngày tải lên: 06/08/2014, 18:21

... function transiently. To inhibit miRNA expression in C. elegans, Zamore’s group injected 2’-oxy- methyl oligonucleotides into developing embryos [11]. eir embryo injection technique is technically ... demonstrate specific inhibition of lys-6 miRNA in the ASEL neurons, where it functions to maintain left-right symmetry. is technique also successfully demonstrated the inhibition in...

Ngày tải lên: 06/08/2014, 19:21

...  for them using bootstrapping, but letting the lower bound of  be as determined in ELM. The  for the remaining predictors are fixed at the determined value from ELM. 4. Repeat step 3 for ... of an individual is of interest in many cases, favouring the first way of modelling the gen- otypic data in Gianola et al. [4]. For example, one might think of classifying individuals wit...

Ngày tải lên: 14/08/2014, 13:21

... BioRad, Hercules, CA). Cytokine determination Serum cytokines [Interleukin-2 (IL-2), Interferon gamma (IFN-γ), Interleukin-4 (IL-4), Interluekin-10 (IL-10)] were determined by sandwich ELISA (Bio- source, ... followed were in accordance with institutional guidelines involving human subjects. Vaccine description All adults were vaccinated with Influenza Virus Vaccine Fluzone ® (in...

Ngày tải lên: 25/10/2012, 11:10

... and emetine, a protein synthesis inhibitor. As seen in Fig. 5B, short -term ERK activation in response to a 15 min PMA treatment alone was, as expected, unaffected by emetine. However, in the presence ... could participate in a process that results in the modification of a pre-existing protein, leading to protection from cadmium toxicity. PKC activation results in a decrease in...

Ngày tải lên: 23/03/2014, 06:20