Báo cáo y học: " Koala retrovirus: a genome invasion in real time" ppsx

... absent from koalas on Kangaroo Island off the coast of South Australia. This island was stocked with koalas in the early part of the twentieth century and has remained essentially isolated since then; ... present, at variable copy number, in the germline of all koalas found in Queensland, but that animals from some areas of southern Australia lack the provirus. Most notably, KoRV appear...

Ngày tải lên: 14/08/2014, 17:22

... We report a new ultra sensitive real time PCR molecular beacon based assay with remarkable analytical and clinical sensitivity, calibrated against the WHO 1 st International standard. Background Chronic ... statistical analysis; AH was the study coordinator and participated to the writing and editing of the manuscript. All authors read and approved the final manuscript. Competing interest...

Ngày tải lên: 12/08/2014, 04:20

... an active complex that mediates an intracellular event (for example, activation of adenylate cyclase). Cocchi et al. Annals of General Psychiatry 2010, 9:25 http://www.annals-general-psychiatry.com/content/9/1/25 Page ... platelet adenylate cyclase. Biol Psychiatry 1988, 23:543-559. 28. Garcia-Sevilla JA, Padro D, Giralt MT, Guimon J, Areso P: Alpha 2- adrenoceptor-mediated inhibition of pl...

Ngày tải lên: 08/08/2014, 23:21

... compartmentalization in lymphoid tissues under non-inflammatory conditions [2]. Most chemokines play a role in inflammatory conditions by inducing integrin activation, chemotaxis, and angiogenesis. Apart ... neutralizing antibody against IL-8 was administered in several types of acute inflammatory disease, including lipopolysaccharide/IL-1 induced arthritis. Anti-IL-8 treatment preven...

Ngày tải lên: 09/08/2014, 01:23

... tissue events. In particular, cytokine mediated pathology may be distinct in cartilage and bone as opposed to synovial tissue or draining lymph node. For many given cytokines, establishing tissue ... (interferon-gamma-inducing factor) is produced by osteoblasts and acts via granulocyte/macrophage colony- stimulating factor and not via interferon-gamma to inhibit osteoclast formation. J Ex...

Ngày tải lên: 09/08/2014, 06:22

... But since the clinical his- tory, examination, upper gastrointestinal endoscopy and barium swallow X-ray were suggestive of achalasia cardia, a final diagnosis of achalasia cardia was made and ... classic X-ray findings are not present. Manometry is the gold standard for diagnosing achalasia cardia [1]. In patients with typical radiographic findings of achalasia, the barium study can be u...

Ngày tải lên: 11/08/2014, 19:21

... artery injury from humeral neck fracture: A rare but disabling traumatic event. Vasc Endovascular Surgery 2004, 38:175-184. 2. Husain AK, Khandeparkar JM, Tendolkar AG, Magotra RA, Parulkar GB: ... humerus. Case presentation: We present a case of axillary artery pseudoaneurysm secondary to such a fracture, in a 82-year-old white woman, presenting 10 weeks after the initial injury, s...

Ngày tải lên: 11/08/2014, 21:22

... and another PCR analysis was performed using the same primers as in the initial analysis. DNA of organs and tissue supernatants was extracted using the QiAamp tissue kit according to the manufacturer's ... PCR using the TaKaRa-Ex PCR system (Takara Bio inc., Otsu, Japan) according to the manufacturer's instructions. An amplification product of approximately 3,3 kb size was obtaine...

Ngày tải lên: 12/08/2014, 04:21

... leukaemia virus-related virus) is a gammaretrovirus that has been detected in human patients with prostate carcinoma, chronic fatigue syndrome (CFS) and also in a small percentage of clinically healthy ... was detected in 8 of 20 of these patients (40%) using a DNA microarray and RT-PCR analysis. In heterozygous patients and patients without the muta- tion, XMRV was found only...

Ngày tải lên: 12/08/2014, 23:23

... alternatively spliced and polyadenylated. Retrovirology 2006, 3:15. 59. Murata K, Hayashibara T, Sugahara K, Uemura A, Yamaguchi T, Harasawa H, Hasegawa H, Tsuruda K, Okazaki T, Koji T, Miyanishi T, Yamada ... 5′-GCATGACACAGG CAAGCATCGAAA; ACTB-F, 5′-ACCAACTGGGACGA- CATGGAGAAA; ACTBR, 5′ -TAGCACAGCCTGGA- TAGCAACGTA. The DKK1b primer pair was used for standard PCR amplification of cDNA prepar...

Ngày tải lên: 13/08/2014, 01:20