Báo cáo y học: "Signals and systems" pot

... cellular signal- ing and more genomic and systematic approaches to biology are becoming increasingly blurred, and the recent Keystone conference on signaling networks reflected an expanded view of signaling. ... spectrometry. Although proteomic studies have been previously published in budding yeast, the current studies attempt to tag and purify approximately 10 times as many unique p...

Ngày tải lên: 14/08/2014, 16:21

... 0.05; Fig. 4a). The ability of the culture-expanded cells to form collagens in the pellet model was analysed biochemically and by immuno- reactivity to type I and type II collagen (Fig. 3). The ... stained positive at days 7 and 14. The other samples were only weakly positive at day 14. To test the potential of using Hyaff-11 as a scaffold for the delivery of chondrocytes, the scaffold...

Ngày tải lên: 09/08/2014, 06:22

... pulmonary instillation [25]. Additionally, cells treated with PM 10 in vitro show activation of NF-κB and AP-1 by pathways that involve the generation of hydroxyl radicals by Fenton-type chemistry [26]. ... constriction and airway inflammation, and direct stimulation of lipid mediators and mucus, which contribute to airway narrowing and blockage respectively. The role of oxidative s...

Ngày tải lên: 12/08/2014, 18:20

... BMPs combine with type 1 (Alk2, Alk3, and Alk6) and type 2 (BR2, ActR2, and ActR2B) receptors, activate the Smad and p38/MAPK signal transduction pathways, and, finally, activate transcription ... in athymic nude (AN) rats. Bone volumes induced by these BMP vectors were greatest when ADhBMPs 4, 6, and 9 were used, followed by ADhBMP2 and, finally, by ADhBMP7. The osteogeni...

Ngày tải lên: 31/10/2012, 17:08

... anandamide and 2-arachidonoylglycerol (2-AG), the cannabinoid CB 1 and CB 2 receptors, and one of the enzymes mostly responsible for endocannabinoid hydrolysis, the fatty acid amide hydrolase (FAAH). By ... cannabinoid system is present in human dendritic cells and can be regulated by cell activation. Keywords: anandamide; 2-arachidonoylglycerol; cannabi- noid; receptor; fatty acid am...

Ngày tải lên: 22/02/2014, 07:20

... cells were examined by ¯uorescence microscopy and the images were acquired by using a Spot-2 cooled camera (Diagnostic Instruments). Sterol D14-reductase assay D14-SR activity was assayed in microsomes ... been hypothesized to originate from D14-SR de®ciency [14]. In addition, interest in the C29D 8,14,24 and C29D 8,24 sterols has been consid- erably stimulated by the ®nding that they pla...

Ngày tải lên: 08/03/2014, 16:20

... enzymatic systems able to activate aromatic rings by mono- and di-hydroxylations and to operate ortho or meta-cleavage pathway [1,2] which leads to citric acid cycle intermediates. Toluene/o-xylene-monooxygenase ... monooxygenation reactions [3]. Thus Tomo is able to oxidize o-, m-andp-xylene, 2,3- and 3,4-dimethyl- phenol, toluene, cresols, benzene, naphthalene, ethylben- zene, styrene...

Ngày tải lên: 17/03/2014, 10:20

... arrested by nocodazoleandthenreleasedintoG1andSphaseasmonitoredby FACS analysis (A). Soluble n uclear and chromatin-bou nd proteins (450 m M NaCl) were investigated by denaturing polyacrylamide ... dissociates and eventually disappears from the nucleus. The data suggest a surprisingly dynamic behaviour ofCdc6pintheHeLacellcycle. Keywords: cell cycle; DNA replication; hCdc6; phospho- rylat...

Ngày tải lên: 17/03/2014, 23:20

... 1. Enzymatic activity of DpAR1 and DpAR2 recombinant pro- teins. Data are mean values (± SD) of triplicate assays. Substrate Enzymatic activity (UÆmg )1 protein) DpAR1 DpAR2 Glyceraldehyde 1.81 ... problem commonly connected with AKR enzymes is their broad substrate specificity, which makes it difficult to determine the physiologically used substrate(s) and consequently the physiological ro...

Ngày tải lên: 18/03/2014, 01:20

... from liver cytosolic triacylglycerol through hydrolysis and reesterifica- tion, and possibly by lipolysis from cellular phospholipids [29,30,35] The major source of chylomicron triacylglycerol is ... (at 2-monoacylglycerol level) of phosphatidic acid and 2-mono- glycerol pathways of synthesis of chylomicron triacylglycerols. J. Lipid Res. 32, 1173–1186. 27. Yang, L .Y. , Kuksis, A. &...

Ngày tải lên: 23/03/2014, 21:20