Báo cáo y học: "TANGOing along the protein secretion pathway" pps

... in the S2 cell model system with which Bard et al. [12] have looked into another fundamental cellular process - protein secretion. The secretory pathway The secretory pathway is the compulsory ... research Minireview TANGOing along the protein secretion pathway Catherine Rabouille and Vangelis Kondylis Address: University Medical Center Utrecht, The Cell Microscopy Center...

Ngày tải lên: 14/08/2014, 16:21

... purposes) ery of leukocytes attends highly active antiretroviral ther- apy (HAART) [12]. Some of these changes may be related to release of sequestered lymphocytes from secondary lymphoid tissues ... cells, most often by killer T cells. The virus-infected cells are predominately memory T cells with the phenotype CD4+CD45RA- or CD4+CD45RA+Fas and are primarily killed by cytotoxic T cells...

Ngày tải lên: 12/08/2014, 23:22

Ngày tải lên: 13/08/2014, 20:22

... chromatography- tandem mass spectrometry (LC-MS/MS) methods. These experiments revealed new specificities for the kinase inhibitors for enzymes they were not initially targeted to. These surprising ... Describing the application of these tools to sphingolipid metabolism, Merrill reported that when serine palmitoyltransferase was stably overexpressed (this is the first enzyme of the sp...

Ngày tải lên: 14/08/2014, 17:22

... of the primary or the secondary antibody or primary incubation in the presence of non-immunized rabbit serum instead of the primary antibody. Cell quantification and Statistical analysis The ... planus (OLP) may present anywhere in the oral cavity. The buccal mucosa, tongue and gingival are the most common sites, whereas palatal lesions are uncommon [3]. They are usually...

Ngày tải lên: 03/11/2012, 09:54

... temperature prior to SDS/PAGE. The Na + /H + exchanger from rat myocyte proteins was immunoprecipitated with a rabbit polyclonal antibody against the cytoplasmic domain of the protein as described earlier ... 8–12. The higher density fractions again contained the majority of the total protein present in the membranes (not shown). To confirm that the higher density fractions...

Ngày tải lên: 08/03/2014, 16:20

... synthesis. On e of the most detailed investigations of fat body proteins has b een the metab- olism of the storage protein arylphorin, which belongs t o the class of hexamerins [5–9]. These proteins ... interrupts the synthesis of a two-hybrid fusion protein, if the f ull-lenth c DNA is ligated in the library plasmid. Stage-specific and tissue-specific appearance of AFP We test...

Ngày tải lên: 08/03/2014, 22:20

... min by measuring pixel inten- sity of the highest band at each time point relative to fully phosphorylated wild-type PAG (Fig. 4B). The wild-type PAG protein was rapidly phosphorylated by Fyn, ... reduced Fyn SH3-domain affinity showed a significantly delayed tyrosine phosphorylation. Phos- phorylation of the PAG-RLP* protein most likely fol- lows a distributive course caused by rando...

Ngày tải lên: 24/03/2014, 00:21

... recog- nized only Cry1Aa toxin but not Cry1Ab, Cry1Ac or Cry9Da2 toxins (Fig. 4Bc). Monoclonal antibody 2A11 recognized Cry1Aa1 toxin and the 55 kDa protein of the activated Cry9Da2 toxin, but not Cry1Ab8 ... Cry1Ab8 or Cry1Ac1 toxins (Fig. 4Bb). Comparison of the domain II regions of Cry1Aa1, Cry1Ab8, Cry1Ac1 and Cry9Da2 toxins The sequences of Cry1Aa1 [40], Cry1Ab8 [41], Cry1Ac1 [...

Ngày tải lên: 24/03/2014, 00:21

... 3¢,3¢-diaminobenzidine hydrochloride c hromogen (Sigma). The speci®city of the method was tested by the omission of the primary antibody in the staining, and the use of p reimmune serum instead of the ®rst ... of adiposity by respiratory uncoupling in adipocytes may be limited by mitochondrial oxidative capacity. Importantly, it has been sho wn in vitro [42] that the uncouplin...

Ngày tải lên: 31/03/2014, 15:20