Báo cáo y học: "A catalog of stability-associated sequence elements in 3'''''''' UTRs of yeast mRNAs" ppt

... ‡ Protein yield of one typical protein preparation. § As determined from measurements of purified protein preparations of 0.2-1 mg/ml. ¶ Melting temperature determined by DSC. The number of independent ... grown in SB media (see above) containing 50 mg/ml ampicillin and 10 mg/ml kanamycin in 5 l baffle shaker flasks in 2 l volumes or in a 5 l fermenter to a cell density of...

Ngày tải lên: 14/08/2014, 14:21

... properly cited. Sequence elements associated with mRNA stability<p>By analyzing 3' UTR sequences and mRNA decay profiles in yeast, 53 sequence motifs have been identified that may be ... Puf fam- ily RNA-binding proteins in yeast. PLoS Biol 2004, 2:E79. 28. Lieb JD, Liu X, Botstein D, Brown PO: Promoter-specific binding of Rap1 revealed by genome-wide maps of protei...

Ngày tải lên: 14/08/2014, 14:22

... standard. RESULTS Induction of citral lyase Initially, the reproducibility of the results was hindered by variations in the citral lyase activity of the P. digitatum spores. Remarkably, lyase activity was ... citral lyase could be induced in the nonger- minating spores of P. digitatum. To the best of our knowledge, the induction of an enzymatic activity in nongerminating spo...

Ngày tải lên: 21/02/2014, 01:21

... phosphotyrosines bind to the phosphotyrosine binding pocket of the SH2 domain, with R94 only playing a minor role in the binding of these peptides. DISCUSSION The cytoplasmic part of the EpoR contains ... tyrosines in the context of SOCS-binding we deter- mined binding affinities of SOCS-3 to peptides containing only phosphotyrosine pY429 or pY431, as well as a double- phosphoryl...

Ngày tải lên: 24/03/2014, 00:21

... of birtoxin (Fig. 4). In ikitoxin the presence of Glu23 charge preceding the a-helix modifies the activity of this toxin in a unique way to result in reduced potency in mice. The C-termini of scorpion ... diversity in scorpion toxins. However, the exact mechanism(s) of diversifying peptide toxins is yet to emerge. Clearly, making small changes in peptide sequences is a me...

Ngày tải lên: 31/03/2014, 08:20

... osteocal- cin and bone formation. The findings suggest a defect in bone formation that may be explained, in part, by systemic inflamma- tion. Indeed, in one of our previous studies [16], uncoupling between ... observed only in bone resorption markers (urinary excretion of N-telopeptide of type I collagen and deoxypyridinoline) [18]. No effect on serum bone alkaline phosphatase,...

Ngày tải lên: 09/08/2014, 10:20

... memory defects in mouse models, suggesting that trisomy of these genes may contribute to learning disability in DS patients [13- 15]. Many phenotypic features of DS are determined very early in ... conse- quence of a deficit in tumour angiogenesis arising from suppression of the calcineurin pathway [12]. Overexpres- sion of a number of HSA21 genes, including Dyrk1a, Synj1...

Ngày tải lên: 09/08/2014, 20:22

... the site of involvement, the size of the cyst, and the pressure caused b y the enlarged cyst [1]. Usually, it presents as an inert, painless, non-inflammatory mass without any deterioration of the ... mechanism of infection [6]. In our case, the hydatid cyst was located subcuta- neously. The patient had not undergone previou s sur- gery for any hydatid cysts, which were never foun...

Ngày tải lên: 10/08/2014, 23:21

... Bond* †1 Address: 1 Moyne Institute for Preventive Medicine, Department of Microbiology, University of Dublin, Trinity College, Dublin 2, Ireland, 2 Institute of Immunology & Department of Biology, National ... phages displaying the DSP peptide specifically bind to a synthetic biotinylated TMG peptide (Fig. 1C, TMG). Conversely, phages displaying the TMG peptide bind to a synthe...

Ngày tải lên: 11/08/2014, 10:23

... movements of the finger and hand. Introduction Intravenous administration of phenytoin can result in soft tissue injury at the site of injection leading to oedema and purplish-black discolouration of ... Although 20 G intravenous Cannula inserted into the interscalene groove for continuous infusion of bupivacaine (0.1%) with fentanyl (2 μg ml-1) Figure 2 20 G intravenous Cannul...

Ngày tải lên: 11/08/2014, 11:22