Báo cáo y học: "Phosphoproteomics: new insights into cellular signaling" pptx

... and the discovery of new phos- phorylation sites in known signaling proteins. As enzymatic catalysis by protein kinases is the only known physiological mechanism for phosphorylation of serine, ... The availabil- ity of genome databases and advancements in analytical technology, especially mass spectrometry, has made it possible to study many phosphoproteins and phosphorylation sites at ......

Ngày tải lên: 14/08/2014, 14:22

... metabolism t hat affect methylation, such as the inhibition of the methylation reactions by S-adenosylhomocysteine and the inhibition of glycine methyl- tranferase by 5-methyltetrahydrofolate. The fact ... University, 722 West 168th Street, New York, NY 10032, USA. 4 Department of Environmental Health Sciences, Mailman School of Public Health, Columbia University, 722 West 168th Street, Ne...

Ngày tải lên: 13/08/2014, 16:20

... complete redundancy, quantitative redun- dancy, and incongruent redundancy. e latter category was unexpected yet is relatively common and can arise from partial redundancy between two factors ... treatment. e sensi- tivity of this approach meant that they could identify about tenfold more protein-coding genes rapidly regu lated by estrogen than did microarrays. Moreover, the data revea...

Ngày tải lên: 09/08/2014, 20:22

... a trusted quality and may also summarise the evidence, and/or host expert discussions about it. This journal may well serve the new dissemination requirements of innovative ways to develop new therapies ... Central and other full-text repositories. A widely accessible journal of this type may become a key resource for the worldwide sarcoma com- munity, filling some of the gaps in medical...

Ngày tải lên: 13/08/2014, 15:21

... interaction analysis for proteome-wide efforts. Tony Pawson (University of Toronto, Canada) is using a combination of classical cell biology techniques with quanti- tative mass spectrometry to study signaling ... the difficulty of a gene-centric approach for mass spectrometry, whereas such an approach is more feasible for antibody- and interaction-based analysis. The shortcomings of previous...

Ngày tải lên: 14/08/2014, 21:20

... photoreceptor types in the retina, although both are also present in pinealocytes. The intracellular concentra- tion of rod arrestin in rod photoreceptors is enormous (over 100 ␮M) [58]. Virtually every mammalian ... predomi- nantly cytoplasmic, whereas the subcellular distribution of arrestin2 varies: for example, it is more abundant in the cytoplasm of striatal neural precursors and neurons...

Ngày tải lên: 14/08/2014, 17:22

... respiratory distress syndrome. New Horiz 1993, 1:593-602. 10. Hannan EL, Siu AL, Kumar D, Kilburn H Jr, Chassin MR: The decline in coronary artery bypass graft surgery mortality in New York State. ... administration or by key members of the health care system. For example, although the clinicians may wish to use a new and highly effective therapy, the pharmacy may be reluctant to stock...

Ngày tải lên: 12/08/2014, 18:21

... transferred N-acetylgalactosamine into the second Thr residue only when N-acetylgalactosamine was attached to the third one. Keywords: O-glycosylation; mucin; polypeptide N-acetylga- lactosaminyltransferase; ... UDP-GalNAc. Biosynthesis of O-glycans is mediated by the step-wise addition of monosaccharides by a variety of glycosyl- transferases, where topology and kinetic properties of Golgi...

Ngày tải lên: 17/03/2014, 10:20

... SOCS-3. Peptide K d (l M ) pY343 > 30 pY401 9.5 ± 0.12 pY429pY431 1.1 ± 0.03 F429pY431 4.6 ± 0.02 pY429F431 4.9 ± 0.02 Y4 2 9Y4 31 ND pY443 ND pY461pY464 ND Y4 61pY464 ND pY46 1Y4 64 ND pY479 ND Ó FEBS 2002 ... L93A and R94E with the peptide pY429pY431 (pYpY). (B) Precipitation of SOCS-3 WT or SOCS-3 R94E with the peptides pY429pY431 (pYpY), pY429F431 (pYF) and F429pY431 (FpY). 2522 M. H...

Ngày tải lên: 24/03/2014, 00:21

... mutation at the 597 position), GST– cyto -Y6 41F (Y! F mutation at the 641 position), GST– cyto -Y6 67F (Y !F mutation at the 667 position), GST– cyto -Y6 91F (Y! F mutation at the 691 position), and ... fusion constructs. Tyrosine phosphorylation (minus GST alone) of GST– cyto-wt with individual tyrosine kinases in a cell-free format.Tyrosine phosphorylation of GST–cyto-wt and GST–cytoY !F...

Ngày tải lên: 24/03/2014, 04:21