Báo cáo y học: "enomic analysis of early murine mammary gland development using novel probe-level algorithms" pdf

... characterization of early mammary gland development The goal of these methodological developments has been the elucidation of biological mechanisms underlying mammary gland development and carcinogenesis. ... RB, Chodosh LA: A novel doxycycline-inducible system for the transgenic analysis of mammary gland biology. FASEB J 2002, 16:283-292. 41. Marquis ST, Rajan J...

Ngày tải lên: 14/08/2014, 14:21

... transcripts not previously detected by microarray analysis in NB4 cellsFigure 3 (see previous page) RACE sequencing can detect transcripts not previously detected by microarray analysis in NB4 cells. ... transcript may be an artifact due to reverse transcription, we employed a novel strategy, namely direct chemical labeling of RNA fol- lowed by strand-specific oligonucleotide tiling m...

Ngày tải lên: 14/08/2014, 08:20

... cancers. Gynecol Oncol 2005, 97:206-213. 65. Doi J, Takemori H, Ohta M, Nonaka Y, Okamoto M: Differential regulation of 3beta-hydroxysteroid dehydrogenase type II and 17alpha-hydroxylase/lyase P450 ... Biology 2006, 7:R48 Meta -analysis of enrichments Enrichment of GO or TF binding sites in different gene groups can depend nonlinearly on the parameters used to define sig- nificantly d...

Ngày tải lên: 14/08/2014, 16:21

... biology and biochemistry have enabled analy- sis of the expression profiles of numerous proteins at once. In this study, we looked at hypoxia-induced apoptosis and, by using the Western array ... to identify key molecules in the course of hy- poxia-induced apoptotic pathways. Such analysis of trophoblastic cells will, in the near future, yield in- sights into the mechanisms o...

Ngày tải lên: 31/10/2012, 15:12

... Big Dye Terminator system (Applied Biosystems, Germany). Sequences were analyzed using the Chromas software (Technely- sium Pty Ltd, Tewantin, Australia) and GeneDoc software (http://www.psc.edu/biomed/genedoc ). ... of hCDC4 can also be observed in Acute Myeloid Leukemia (AML) or high risk Myelodysplastic Syndrome (MDS). Therefore we carried out a mutational analysis of the hCD...

Ngày tải lên: 31/10/2012, 16:49

... levels of many of these genes identified in the array analysis. Moreover, Western blot analysis of thymo- cyte lysates also confirmed that IgM increases with age (Figure 3). Flow cytometry analysis ... and array analysis. For each array sample, the RNA was prepared from the purified thymocytes. The thymocytes were processed by using the RNEasy Mini Kit (Qiagen, Valencia, CA...

Ngày tải lên: 03/11/2012, 11:35

... three domains and are present in the inhibitory receptors of Fig. 6. Inhibition of in vitro cytotoxicity of myotoxins I or II by DM64. Cytotoxicity was analyzed in vitro using C2C12 skeletal muscle cells. Toxins ... database using BLAST Search Program (NCBI, Bethesda, MD). Myotoxicity in vivo Myotoxicity was analyzed by quantification of plasma creatine kinase (CK) activity using...

Ngày tải lên: 21/02/2014, 01:21

... H.S., Han, Y. & Fung, B.K K. (2000) Subunit e xchange of small h eat sho ck proteins. Analysis of olig- omer formation of aA-crystallin and Hsp27 by fluorescence res- onance energy transfer ... chaperoning in vitro [66], and disassembly of active units from an oligomeric (storage) state of a-crystallin was proposed, upon structural analysis of aA-crystallin by site- direct...

Ngày tải lên: 22/02/2014, 04:20

... formation of gamma glutamyl-lysine bonds [4]. Furthermore, tTG plays a role in the transduction of extracellular signals, mediated by its additional GTP- hydrolyzing activity [5], analogous to that of ... member of a family of seven isoforms of enzymes involved in protein cross-linking, including prostatic TGase and factor XIII. tTG is a Ca 2+ -dependent ubiquitous intracellular e...

Ngày tải lên: 08/03/2014, 09:20

... C-1, and was mostly present in an a-pyranose form. Acyl 1 ,acyl 2 and acyl 3 residues had hydroxy or acyloxy groups at C-3 ( 13 C signals of C-3 at 69.0–72.4 p.p.m.), while acyl 4 had no substituents. The ... 2-N-(3-O- acyl 4 -acyl 2 )-b-GlcN-(1–6)-2-N-acyl 1 )3-O-acyl 3 -GlcN where acyl 1 ,acyl 2 and acyl 3 are 3-hydroxyhexadecanoic or 3-hy- droxyoctadecanoic acids, acyl 4 is tetradecanoic...

Ngày tải lên: 08/03/2014, 09:20