analysis of genes and genomes phần 9 ppsx

... Mol. Biol., 2 19, 45– 59. Dunham, I. et al. ( 199 9) The DNA sequence of human chromosome 22. Nature, 402, 4 89 495 . 414 NOBEL PRIZE WINNERS 198 9 Sidney Altman and Thomas R. Cech for their discovery of catalytic ... N., Boehmelt, G. and Iscove, N. ( 199 5) Analysis of gene expression in a complex differentiation hierarchy by global amplification of cDNA from single cells. Cur...

Ngày tải lên: 14/08/2014, 11:21

... suggested by Watson and Crick. The separation of the DNA strands of the parent helix (black) means that each strand can act as a template of the synthesis of a new strand of DNA (blue). This ... Meselson and Stahl, however, dismissed this mode of replication by the analysis of individual single strands of replicated DNA. They heated their DNA (Figure 1.11) and found th...

Ngày tải lên: 14/08/2014, 11:21

... number and inter-pulse interval) (Ho and Mittal, 199 6; Canatella et al., 2001). Two main factors seem to influence the formation of electropores – the types of cell that are used, and the amplitude and ... transformation efficiencies of 10 7 –10 9 transformants per microgram of DNA added being achieved, depending on the particular strain of E. coli being employed (Liu and...

Ngày tải lên: 14/08/2014, 11:21

... between 17 and 30 nucleotides in length, which flank the DNA target sequence that is to be copied. One of the primers is the same sequence as one strand of the DNA Analysis of Genes and Genomes Richard ... ends of the + strand are ligated to form the single-stranded genome. The switch between the double-stranded RF form and the single-stranded + form of the M13 viral geno...

Ngày tải lên: 14/08/2014, 11:21

... screening of a large number of bacterial colonies from a single plate, and also utilizes a filter lift procedure (Grunstein and Hogness, 197 5; Grunstein and Wallis, 197 9; Hanahan and Meselson, 198 0). ... bait and prey, in yeast (Fields and Song, 198 9). The screen was performed in a yeast strain that was deleted for the wild-type copies of the GAL4 and GAL80 genes, an...

Ngày tải lên: 14/08/2014, 11:21

... al., 199 2; Marmorstein and Harrison, 199 4; Swaminathan et al., 199 7) and are shown in Figure 7.7(c)–(e). Each protein–DNA complex shows the same overall format. Each protein is dimeric and makes ... pro- duction. The architecture of a typical expression vector is shown in Figure 8.1. Analysis of Genes and Genomes Richard J. Reece  2004 John Wiley & Sons, Ltd ISBNs:...

Ngày tải lên: 14/08/2014, 11:21

... function. The analysis of changes in the expression of all of the thousands or tens of thousands of genes within a genome is essential if we are to understand the interplay between genes and gene ... different genes – representing almost 20 per cent of the total number of genes (Chu et al., 199 8). In other cases, changes in cellular environment may only alter the ex...

Ngày tải lên: 14/08/2014, 11:21

... one containing the genes required for the production of single-stranded AAV genomic DNA, and the other bearing the adenoviral genes required for lytic replication and packaging (Matsushita et al., 199 8). The ... from Birch ( 199 7) Crop and release date Name Company Novel properties Tomato ( 199 4) Flavr Savr Calgene Vine-ripened flavour, increased shelf life Tomato ( 199 5) Zenec...

Ngày tải lên: 14/08/2014, 11:21

... Gene, 111, 199 –206. Ho, S.Y. and Mittal, G.S. ( 199 6) Electroporation of cell membranes: a review. Crit. Rev. Biotechnol., 16, 3 49 362. Hoffman, A. and Roeder, R.G. ( 199 1) Purification of his-tagged ... S.P. and Rigby, P.W. ( 199 3) The regulation of myogenin gene expression during the embryonic development of the mouse. Genes Dev., 7, 1277–12 89. Yie, Y., Wei, Z. and T...

Ngày tải lên: 14/08/2014, 11:21