analysis of genes and genomes phần 6 pps

... initial treatment of the mutagenesis reaction as two separate components means 8.3 EXPRESSION IN INSECT CELLS 271 ORF603 ORF 162 9 polyH RERE RE ORF603 polyH RE RE ORF603 ORF 162 9 ori AMP R lac Z Target ORF603 ... protein–protein interactions than mutagenesis of other residues. Two approaches are commonly used for the creation of random mutations within individual genes, or parts of...

Ngày tải lên: 14/08/2014, 11:21

... (1972) Nature of ColE1 plasmid replication in Escherichia coli in the presence of chloramphenicol. J. Bacteriol., 110, 66 7 67 6. Cline, J., Braman, J.C. and Hogrefe, H.H. (19 96) PCR fidelity of Pfu DNA ... contributions to the establishment of oligonucleotide based, site directed mutagenesis and its development for protein studies (Chemistry) Analysis of Genes and Genom...

Ngày tải lên: 14/08/2014, 11:21

... suggested by Watson and Crick. The separation of the DNA strands of the parent helix (black) means that each strand can act as a template of the synthesis of a new strand of DNA (blue). This ... Meselson and Stahl, however, dismissed this mode of replication by the analysis of individual single strands of replicated DNA. They heated their DNA (Figure 1.11) and found th...

Ngày tải lên: 14/08/2014, 11:21

... Chapter 6. 2 .6. 2 The Compass Points of Blotting Since Southern’s first description of blotting and hybridization, a number of variants have been described. These all involve the transfer of material ... number and inter-pulse interval) (Ho and Mittal, 19 96; Canatella et al., 2001). Two main factors seem to influence the formation of electropores – the types of cell that a...

Ngày tải lên: 14/08/2014, 11:21

... between 17 and 30 nucleotides in length, which flank the DNA target sequence that is to be copied. One of the primers is the same sequence as one strand of the DNA Analysis of Genes and Genomes Richard ... ends of the + strand are ligated to form the single-stranded genome. The switch between the double-stranded RF form and the single-stranded + form of the M13 viral geno...

Ngày tải lên: 14/08/2014, 11:21

... a biochemical function of the polypeptide or • the ability of the polypeptide encoded by the recombinant clone to interact with other polypeptides. Analysis of Genes and Genomes Richard J. Reece  ... bait and prey, in yeast (Fields and Song, 1989). The screen was performed in a yeast strain that was deleted for the wild-type copies of the GAL4 and GAL80 genes, and th...

Ngày tải lên: 14/08/2014, 11:21

... function. The analysis of changes in the expression of all of the thousands or tens of thousands of genes within a genome is essential if we are to understand the interplay between genes and gene ... in expression of genes within the tissue samples can be detected through the analysis of the intensity and individual bands. (b) Differential display of four RNA sam...

Ngày tải lên: 14/08/2014, 11:21

... number of genes into a small genome, the virus utilizes a number of strategies such as splicing and ribosomal frame- shifting (Farabaugh, 19 96) . For example, the pro gene often overlaps gag and/ or ... strong selection Further rounds of regeneration and selection Figure 11 .6. Selection of homogenous transgenic chloroplasts. Plant cells can contain between 50 and 100 chloro...

Ngày tải lên: 14/08/2014, 11:21

... immunoprecipi- tation and CpG island microarray analysis. Genes Dev., 16, 235–244. Weiss, B. and Richardson, C.C. (1 967 ) Enzymatic breakage and joining of deoxyri- bonucleic acid, I. Repair of single-strand breaks ... P., Challberg, M.D. and Kelly, T.J. (2001) Assembly of the human origin recognition complex. J. Biol. Chem., 2 76, 26 666 – 26 673. Vasquez, J.R., Evnin, L....

Ngày tải lên: 14/08/2014, 11:21