analysis of genes and genomes phần 5 potx

... POLYMERASE CHAIN REACTION 4 3′ 5 3′ 5 3′ 5 3′ 5 3′ 5 Primer 1 5 3′ QR Primer 2 Probe 5 3′ 3′ 5 3′ 5 5 5 Q R 3′ 3′ 5 3′ 5 5 5 Q R 3′ 3′ 5 3′ 5 5 5 Q R Displacement Cleavage Completed synthesis Primer ... a biochemical function of the polypeptide or • the ability of the polypeptide encoded by the recombinant clone to interact with other polypeptides. Analysis...

Ngày tải lên: 14/08/2014, 11:21

... between 17 and 30 nucleotides in length, which flank the DNA target sequence that is to be copied. One of the primers is the same sequence as one strand of the DNA Analysis of Genes and Genomes Richard ... 2 2 2 Extension temperature ( ◦ C) 70– 75 70–74 70– 75 70– 75 70– 75 [Magnesium] (mM) 1–4 1–4 2–4 2–4 2–4 pH @ 25 ◦ C 7.0–7 .5 7.0–7 .5 8.0–9.0 7.0–7 .5 7.0–7 .5 [dN...

Ngày tải lên: 14/08/2014, 11:21

... 75 G A AT T C CTTAAG 5 3′ 3′ 5 G CT A TAG ATATC 5 3′ 3′ 5 CTGCAG G A CGTC 5 3′ 3′ 5 EcoRV PstI G CTTAA 5 3′ 3′ 5 AATTC G 5 3′ 3′ 5 + 5 overhang ends EcoRI C G T T A A 5 3′ 3′ 5 5 3′ A TAG 3′ 5 + Blunt ... suggested by Watson and Crick. The separation of the DNA strands of the parent helix (black) means that each strand can act as a template of the synthesis...

Ngày tải lên: 14/08/2014, 11:21

... 2.13). 10000 Size (bp) Distance migrated (mm) 8000 6000 50 00 4000 350 0 3000 250 0 2000 150 0 1000 750 50 0 250 10000 12.00 8000 12. 85 6000 14 .55 50 00 16.12 4000 18.24 350 0 19.74 3000 21.86 250 0 23.97 2000 27.32 150 0 31.72 1000 38.79 750 43.29 50 0 50 . 25 250 ... 31.72 1000 38.79 750 43.29 50 0 50 . 25 250 59 . 35 − + Direction of DNA migration 1 250 0 10000...

Ngày tải lên: 14/08/2014, 11:21

... mixture of A and a small quantity of the other nucleotide precursors would be added. Such mixtures might commonly contain 95 per cent of the wild-type nucleotide and 1.7 per cent of each of the ... Procedures 0 1020304 050 60708090100 Efficiency of mutation (%) 0 5 10 15 20 Number of clones analysed to give 90% chance of isolating the mutant Figure 7.2. Efficient mutag...

Ngày tải lên: 14/08/2014, 11:21

... function. The analysis of changes in the expression of all of the thousands or tens of thousands of genes within a genome is essential if we are to understand the interplay between genes and gene ... in expression of genes within the tissue samples can be detected through the analysis of the intensity and individual bands. (b) Differential display of four RNA sam...

Ngày tải lên: 14/08/2014, 11:21

... Ara- bidopsis thaliana, is composed of approximately 155 kbp of DNA containing 87 protein coding genes (Sato et al., 1999). Chloroplast genes, like those of bacteria, are generally arranged into ... T-DNA, the Ti plasmid also carries the genes needed for opine utilization, a series of approximately 35 genes required for virulence (vir)andanAgrobacterium origin of replication...

Ngày tải lên: 14/08/2014, 11:21

... G. and Brown, R. (19 85) Use of gene transfer and a novel cosmid rescue strategy to isolate transforming sequences. EMBO J., 4, 258 3– 258 8. Brandhorst, B.P. and McConkey, E.H. (1974) Stability of ... Biol., 85, 451 56 3. Brent, R. and Finley, R.L., Jr. (1997) Understanding gene and allele function with two-hybrid methods. Annu. Rev. Genet., 31, 663–704. Brent, R. and Ptashn...

Ngày tải lên: 14/08/2014, 11:21

... (RISC), 332 RNA polymerase, 34, 47, 51 , 55 , 124, 257 RNA pol I, 47 RNA pol II, 47, 51 , 53 , 191 CTD, 51 , 53 RNA pol III, 47, 51 RNA primer, 34 RNA silencing, 330 RNA splicing, 55 , 310 RNA-dependent RNA polymerase ... Biol., 4, 19 85 1998. Yoshida, M. and Seiki, M. (1987) Recent advances in the molecular biology of HTLV-1: trans-activation of viral and cellular genes....

Ngày tải lên: 14/08/2014, 11:21