analysis of genes and genomes phần 4 potx
analysis of genes and genomes phần 4 potx
... temperature (
◦
C) 70–75 70– 74 70–75 70–75 70–75
[Magnesium] (mM) 1 4 1 4 2 4 2 4 2 4
pH @ 25
◦
C 7.0–7.5 7.0–7.5 8.0–9.0 7.0–7.5 7.0–7.5
[dNTP] (mM) 40 –200 40 –200 40 –200 40 –200 200
[Primers] (mM) ... between 17 and 30
nucleotides in length, which flank the DNA target sequence that is to be
copied. One of the primers is the same sequence as one strand of the DNA
Analysis...
analysis of genes and genomes phần 5 potx
... function of the polypeptide or
• the ability of the polypeptide encoded by the recombinant clone to interact
with other polypeptides.
Analysis of Genes and Genomes Richard J. Reece
20 04 John ... bait and prey,
in yeast (Fields and Song, 1989). The screen was performed in a yeast strain
that was deleted for the wild-type copies of the GAL4 and GAL80 genes, and
the...
analysis of genes and genomes phần 2 doc
... biology. The discovery of enzymes able to cleave DNA at specific
Analysis of Genes and Genomes Richard J. Reece
20 04 John Wiley & Sons, Ltd ISBNs: 0 -47 0- 843 79-9 (HB); 0 -47 0- 843 80-2 (PB)
2.1 RESTRICTION ... suggested by Watson and
Crick. The separation of the DNA strands of the parent helix (black) means that each
strand can act as a template of the synthesis...
analysis of genes and genomes phần 3 pot
... Construction of genomic libraries ∼23 λZAP, EMBL4
Cosmid Construction of genomic libraries 44 pJB8
M13 In vitro mutagenesis
DNA sequencing
8–9 M13mp18
Phagemid General DNA manipulation In vitro
mutagenesis
10–20 ... 2.13).
10000
Size
(bp)
Distance
migrated
(mm)
8000
6000
5000
40 00
3500
3000
2500
2000
1500
1000
750
500
250
10000 12.00
8000 12.85
6000 14. 55
5000 16.12
40 00 18. 24
3500...
analysis of genes and genomes phần 6 pps
... architecture of a typical expression vector is shown in Figure 8.1.
Analysis of Genes and Genomes Richard J. Reece
20 04 John Wiley & Sons, Ltd ISBNs: 0 -47 0- 843 79-9 (HB); 0 -47 0- 843 80-2 (PB)
2 54 CREATING ... sequence. Two example of oligonucleotides
produced are shown in Figure 7.10. By choosing an appropriate level
7 .4 PCR BASED MUTAGENESIS 243
to the anti-se...
analysis of genes and genomes phần 7 pot
... function.
The analysis of changes in the expression of all of the thousands or tens of
thousands of genes within a genome is essential if we are to understand the
interplay between genes and gene ... The
Analysis of Genes and Genomes Richard J. Reece
20 04 John Wiley & Sons, Ltd ISBNs: 0 -47 0- 843 79-9 (HB); 0 -47 0- 843 80-2 (PB)
2 94 GENOME SEQUENCING PR...
analysis of genes and genomes phần 8 pdf
... be induced to reproduce
Analysis of Genes and Genomes Richard J. Reece
20 04 John Wiley & Sons, Ltd ISBNs: 0 -47 0- 843 79-9 (HB); 0 -47 0- 843 80-2 (PB)
328 POST-GENOME ANALYSIS 10
Gene 1
URA3
URA3
Ura
+
PCR ... manipulate using standard techniques. However, the isolation of
a full-length cDNA clone corresponding to entire RNA genomes (Ahlquist
and Janda, 19 84) has...
analysis of genes and genomes phần 9 ppsx
... the fertilized
Analysis of Genes and Genomes Richard J. Reece
20 04 John Wiley & Sons, Ltd ISBNs: 0 -47 0- 843 79-9 (HB); 0 -47 0- 843 80-2 (PB)
40 4 GLOSSARY
Homology – a similarity often attributable ... sequence of human chromosome 22. Nature, 40 2,
48 9 49 5.
41 4 NOBEL PRIZE WINNERS
1989
Sidney Altman and Thomas R. Cech for their discovery of catalytic properti...
analysis of genes and genomes phần 10 pptx
... acids of GAL4 are
recognized by GAL80. Cell, 50, 137– 142 .
Ma, J. and Ptashne, M. (1987b) Deletion analysis of GAL4 defines two transcriptional
activating segments. Cell, 48 , 847 –853.
Ma, J. and ... 133, 141
λEMBL4, 133
λgt11, 1 34
λZAP, 1 34, 141 , 2 14
Bacteriophage P1, 146
Bacteriophage T2, 5
Bacteriophage φX1 74, 296
Baculovirus, 269, 270, 378
Bait, 2 24
Baltimore, David...