analysis of genes and genomes phần 4 potx

... temperature ( ◦ C) 70–75 70– 74 70–75 70–75 70–75 [Magnesium] (mM) 1 4 1 4 2 4 2 4 2 4 pH @ 25 ◦ C 7.0–7.5 7.0–7.5 8.0–9.0 7.0–7.5 7.0–7.5 [dNTP] (mM) 40 –200 40 –200 40 –200 40 –200 200 [Primers] (mM) ... between 17 and 30 nucleotides in length, which flank the DNA target sequence that is to be copied. One of the primers is the same sequence as one strand of the DNA Analysis...

Ngày tải lên: 14/08/2014, 11:21

... function of the polypeptide or • the ability of the polypeptide encoded by the recombinant clone to interact with other polypeptides. Analysis of Genes and Genomes Richard J. Reece  20 04 John ... bait and prey, in yeast (Fields and Song, 1989). The screen was performed in a yeast strain that was deleted for the wild-type copies of the GAL4 and GAL80 genes, and the...

Ngày tải lên: 14/08/2014, 11:21

... biology. The discovery of enzymes able to cleave DNA at specific Analysis of Genes and Genomes Richard J. Reece  20 04 John Wiley & Sons, Ltd ISBNs: 0 -47 0- 843 79-9 (HB); 0 -47 0- 843 80-2 (PB) 2.1 RESTRICTION ... suggested by Watson and Crick. The separation of the DNA strands of the parent helix (black) means that each strand can act as a template of the synthesis...

Ngày tải lên: 14/08/2014, 11:21

... Construction of genomic libraries ∼23 λZAP, EMBL4 Cosmid Construction of genomic libraries 44 pJB8 M13 In vitro mutagenesis DNA sequencing 8–9 M13mp18 Phagemid General DNA manipulation In vitro mutagenesis 10–20 ... 2.13). 10000 Size (bp) Distance migrated (mm) 8000 6000 5000 40 00 3500 3000 2500 2000 1500 1000 750 500 250 10000 12.00 8000 12.85 6000 14. 55 5000 16.12 40 00 18. 24 3500...

Ngày tải lên: 14/08/2014, 11:21

... architecture of a typical expression vector is shown in Figure 8.1. Analysis of Genes and Genomes Richard J. Reece  20 04 John Wiley & Sons, Ltd ISBNs: 0 -47 0- 843 79-9 (HB); 0 -47 0- 843 80-2 (PB) 2 54 CREATING ... sequence. Two example of oligonucleotides produced are shown in Figure 7.10. By choosing an appropriate level 7 .4 PCR BASED MUTAGENESIS 243 to the anti-se...

Ngày tải lên: 14/08/2014, 11:21

... function. The analysis of changes in the expression of all of the thousands or tens of thousands of genes within a genome is essential if we are to understand the interplay between genes and gene ... The Analysis of Genes and Genomes Richard J. Reece  20 04 John Wiley & Sons, Ltd ISBNs: 0 -47 0- 843 79-9 (HB); 0 -47 0- 843 80-2 (PB) 2 94 GENOME SEQUENCING PR...

Ngày tải lên: 14/08/2014, 11:21

... be induced to reproduce Analysis of Genes and Genomes Richard J. Reece  20 04 John Wiley & Sons, Ltd ISBNs: 0 -47 0- 843 79-9 (HB); 0 -47 0- 843 80-2 (PB) 328 POST-GENOME ANALYSIS 10 Gene 1 URA3 URA3 Ura + PCR ... manipulate using standard techniques. However, the isolation of a full-length cDNA clone corresponding to entire RNA genomes (Ahlquist and Janda, 19 84) has...

Ngày tải lên: 14/08/2014, 11:21

... the fertilized Analysis of Genes and Genomes Richard J. Reece  20 04 John Wiley & Sons, Ltd ISBNs: 0 -47 0- 843 79-9 (HB); 0 -47 0- 843 80-2 (PB) 40 4 GLOSSARY Homology – a similarity often attributable ... sequence of human chromosome 22. Nature, 40 2, 48 9 49 5. 41 4 NOBEL PRIZE WINNERS 1989 Sidney Altman and Thomas R. Cech for their discovery of catalytic properti...

Ngày tải lên: 14/08/2014, 11:21

... acids of GAL4 are recognized by GAL80. Cell, 50, 137– 142 . Ma, J. and Ptashne, M. (1987b) Deletion analysis of GAL4 defines two transcriptional activating segments. Cell, 48 , 847 –853. Ma, J. and ... 133, 141 λEMBL4, 133 λgt11, 1 34 λZAP, 1 34, 141 , 2 14 Bacteriophage P1, 146 Bacteriophage T2, 5 Bacteriophage φX1 74, 296 Baculovirus, 269, 270, 378 Bait, 2 24 Baltimore, David...

Ngày tải lên: 14/08/2014, 11:21