analysis of genes and genomes phần 3 pot

... 2. 13) . 10000 Size (bp) Distance migrated (mm) 8000 6000 5000 4000 35 00 30 00 2500 2000 1500 1000 750 500 250 10000 12.00 8000 12.85 6000 14.55 5000 16.12 4000 18.24 35 00 19.74 30 00 21.86 2500 23. 97 2000 27 .32 1500 31 .72 1000 38 .79 750 43. 29 500 50.25 250 ... phosphorylation. 2 .3 THE BASICS OF CLONING 79 GGATCC CCT A G G 5′ 3 GA A T T C CTTAAG 3 3 5′ BamHI EcoRI G G A...

Ngày tải lên: 14/08/2014, 11:21

... between 17 and 30 nucleotides in length, which flank the DNA target sequence that is to be copied. One of the primers is the same sequence as one strand of the DNA Analysis of Genes and Genomes Richard ... (Yanisch-Perron, Vieira and Messing, 1985; Norrander, Kempe and Messing, 19 83) . The RF form of M 13 vectors can be isolated by standard plasmid DNA preparation pro...

Ngày tải lên: 14/08/2014, 11:21

... 0-470-8 437 9-9 (HB); 0-470-8 438 0-2 (PB) 180 POLYMERASE CHAIN REACTION 4 3 5′ 3 5′ 3 5′ 3 5′ 3 5′ Primer 1 5′ 3 QR Primer 2 Probe 5 3 3 5′ 3 5′ 5′ 5′ Q R 3 3 5′ 3 5′ 5′ 5′ Q R 3 3 5′ 3 5′ 5′ 5′ Q R Displacement Cleavage Completed synthesis Primer ... produce the first cDNA strand (Murakawa et al., 1988). The production of a single strand of cDNA is mRNA 5'–...

Ngày tải lên: 14/08/2014, 11:21

... of changes in the expression of all of the thousands or tens of thousands of genes within a genome is essential if we are to understand the interplay between genes and gene products. DNA microarrays ... in expression of genes within the tissue samples can be detected through the analysis of the intensity and individual bands. (b) Differential display of four RNA sa...

Ngày tải lên: 14/08/2014, 11:21

... the lengths of those links but also the locations of the fixed pivots Oz and 04 in the plane and the lengths oflinks 3 and 1. Link 1 is defined as vector G in Figure 5-4 (p. 202) and can be found ... PJ, Pz, and P3. Points A and B are the attachment points for the rockers (links 2 and 4, respectively) on the coupler (link 3) . We wish to solve for the coordinates of vec...

Ngày tải lên: 08/08/2014, 13:20

... 75 G A AT T C CTTAAG 5′ 3 3 5′ G CT A TAG ATATC 5′ 3 3 5′ CTGCAG G A CGTC 5′ 3 3 5′ EcoRV PstI G CTTAA 5′ 3 3 5′ AATTC G 5′ 3 3 5′ + 5′ overhang ends EcoRI C G T T A A 5′ 3 3 5′ 5′ 3 A TAG 3 5′ + Blunt ... one old strand and one new strand 28 DNA: STRUCTURE AND FUNCTION 1 Double-stranded DNA 2 nm 10 nm 30 nm “Beads on a string” Cross-section of 30 nm fibre Mitotic ch...

Ngày tải lên: 14/08/2014, 11:21

... architecture of a typical expression vector is shown in Figure 8.1. Analysis of Genes and Genomes Richard J. Reece  2004 John Wiley & Sons, Ltd ISBNs: 0-470-8 437 9-9 (HB); 0-470-8 438 0-2 (PB) 254 ... mixture of A and a small quantity of the other nucleotide precursors would be added. Such mixtures might commonly contain 95 per cent of the wild-type nucleotide and...

Ngày tải lên: 14/08/2014, 11:21

... reproduce Analysis of Genes and Genomes Richard J. Reece  2004 John Wiley & Sons, Ltd ISBNs: 0-470-8 437 9-9 (HB); 0-470-8 438 0-2 (PB) 32 8 POST-GENOME ANALYSIS 10 Gene 1 URA3 URA3 Ura + PCR ... possess RNA genomes and are therefore difficult to manipulate using standard techniques. However, the isolation of a full-length cDNA clone corresponding to entire RNA genomes...

Ngày tải lên: 14/08/2014, 11:21

... introns and ligating the ends of exons Splicing – the removal of introns and joining of exons to form a mature mRNA 13. 1 PRONUCLEAR INJECTION 38 3 high levels of growth hormone mRNA in their liver and ... the fertilized Analysis of Genes and Genomes Richard J. Reece  2004 John Wiley & Sons, Ltd ISBNs: 0-470-8 437 9-9 (HB); 0-470-8 438 0-2 (PB) 404 GLOSSARY Homo...

Ngày tải lên: 14/08/2014, 11:21