analysis of genes and genomes phần 3 pot
analysis of genes and genomes phần 3 pot
... 2. 13) .
10000
Size
(bp)
Distance
migrated
(mm)
8000
6000
5000
4000
35 00
30 00
2500
2000
1500
1000
750
500
250
10000 12.00
8000 12.85
6000 14.55
5000 16.12
4000 18.24
35 00 19.74
30 00 21.86
2500 23. 97
2000 27 .32
1500 31 .72
1000 38 .79
750 43. 29
500 50.25
250 ... phosphorylation.
2 .3 THE BASICS OF CLONING 79
GGATCC
CCT
A
G
G
5′
3
GA A T T C
CTTAAG
3
3
5′
BamHI
EcoRI
G
G
A...
analysis of genes and genomes phần 4 potx
... between 17 and 30
nucleotides in length, which flank the DNA target sequence that is to be
copied. One of the primers is the same sequence as one strand of the DNA
Analysis of Genes and Genomes Richard ... (Yanisch-Perron, Vieira and Messing, 1985;
Norrander, Kempe and Messing, 19 83) . The RF form of M 13 vectors can be
isolated by standard plasmid DNA preparation pro...
analysis of genes and genomes phần 5 potx
... 0-470-8 437 9-9 (HB); 0-470-8 438 0-2 (PB)
180 POLYMERASE CHAIN REACTION 4
3
5′
3
5′
3
5′
3
5′
3
5′
Primer 1
5′
3
QR
Primer 2
Probe
5 3
3
5′
3
5′
5′
5′
Q
R
3
3
5′
3
5′
5′
5′
Q
R
3
3
5′
3
5′
5′
5′
Q
R
Displacement
Cleavage
Completed
synthesis
Primer ... produce the first cDNA
strand (Murakawa et al., 1988). The production of a single strand of cDNA is
mRNA
5'–...
analysis of genes and genomes phần 7 pot
... of changes in the expression of all of the thousands or tens of
thousands of genes within a genome is essential if we are to understand the
interplay between genes and gene products.
DNA microarrays ... in
expression of genes within the tissue samples can be detected through the analysis of the
intensity and individual bands. (b) Differential display of four RNA sa...
DESIGN OF MACHINERYAN INTRODUCTION TO THE SYNTHESIS AND ANALYSIS OF MECHANISMS AND MACHINES phần 3 pot
... the lengths of those links but also the
locations of the fixed pivots Oz and
04
in the plane and the lengths oflinks 3 and 1. Link
1 is defined as vector G in Figure 5-4 (p. 202) and can be found ... PJ, Pz, and P3. Points A and B are the attachment points for the rockers
(links 2 and 4, respectively) on the coupler (link 3) . We wish to solve for the coordinates
of vec...
analysis of genes and genomes phần 2 doc
... 75
G
A
AT
T
C
CTTAAG
5′
3
3 5′
G
CT
A
TAG
ATATC
5′
3
3 5′
CTGCAG
G
A
CGTC
5′
3
3 5′
EcoRV
PstI
G
CTTAA
5′ 3
3 5′
AATTC
G
5′ 3
3
5′
+
5′ overhang ends
EcoRI
C
G
T
T
A
A
5′
3
3
5′
5′
3
A
TAG
3
5′
+
Blunt ... one old strand and
one new strand
28 DNA: STRUCTURE AND FUNCTION 1
Double-stranded DNA
2 nm
10 nm
30 nm
“Beads on a string”
Cross-section
of 30 nm fibre
Mitotic ch...
analysis of genes and genomes phần 6 pps
... architecture of a typical expression vector is shown in Figure 8.1.
Analysis of Genes and Genomes Richard J. Reece
2004 John Wiley & Sons, Ltd ISBNs: 0-470-8 437 9-9 (HB); 0-470-8 438 0-2 (PB)
254 ... mixture of A and a small quantity
of the other nucleotide precursors would be added. Such mixtures might
commonly contain 95 per cent of the wild-type nucleotide and...
analysis of genes and genomes phần 8 pdf
... reproduce
Analysis of Genes and Genomes Richard J. Reece
2004 John Wiley & Sons, Ltd ISBNs: 0-470-8 437 9-9 (HB); 0-470-8 438 0-2 (PB)
32 8 POST-GENOME ANALYSIS 10
Gene 1
URA3
URA3
Ura
+
PCR ... possess RNA genomes and are therefore
difficult to manipulate using standard techniques. However, the isolation of
a full-length cDNA clone corresponding to entire RNA genomes...
analysis of genes and genomes phần 9 ppsx
... introns and ligating
the ends of exons
Splicing – the removal of introns and joining of exons to form a mature mRNA
13. 1 PRONUCLEAR INJECTION 38 3
high levels of growth hormone mRNA in their liver and ... the fertilized
Analysis of Genes and Genomes Richard J. Reece
2004 John Wiley & Sons, Ltd ISBNs: 0-470-8 437 9-9 (HB); 0-470-8 438 0-2 (PB)
404 GLOSSARY
Homo...