Báo cáo y học: " Cloning and characterization of microRNAs from wheat (Triticum aestivum L )" docx

... * Key Laboratory of Crop Heterosis and Utilization (MOE) and State Key Laboratory for Agrobiotechnology, Key Laboratory of Crop Genomics and Genetic Improvement (MOA), Beijing Key Laboratory of ... below spikes, leaves, flag leaves, roots and spikes, were probed with labeled oligonucleotides. The tRNA and 5S RNA bands were visualized by ethidium bromide staining of polya...

Ngày tải lên: 14/08/2014, 07:21

... resulting from application of calciseptine, a well-characterized neurotoxin from black mamba (Dendroaspis polylepis polylepis).Calciseptineisa known blocker of L- type Ca 2+ channels, a property that underlies ... m M dithiothreitol in the presence of 0.5 M Tris/HCl, pH 8.5, 6 M guanidine hydrochloride, and 2 m M EDTA in a volume of 0.5 mL. Three microliters of 4-vinylpyridin...

Ngày tải lên: 18/03/2014, 01:20

... Nuclear import of glycoconjugates is distinct from the classical NLS pathway. J. Cell Sci. 108, 1325–1332. SUPPLEMENTARY MATERIAL The following material is available from http://www. blackwell-science.com/products/journals/suppmat/EJB/ EJB3232/EJB3232sm.htm Fig. ... was added. Biosynthetic labeling of transfected cells and immunoprecipitation of cell extracts COS-1 cells were cult...

Ngày tải lên: 23/03/2014, 21:20

... by immunofluorescence labelling and confocal microscopy Localization of PsCIPK and PsCBL was analysed by immunofluorescence labelling of tobacco BY2 cells fol- lowed by confocal microscopy. Cell ... partially cloned by PCR and then followed by isolation of full-length clones by library screening. For partial cloning of pea CIPK (PsCIPK) or pea CBL (PsCBL), all the known sequence...

Ngày tải lên: 19/02/2014, 07:20

... in a total volume of 50 lL with KOD-Plus-DNA Polymerase (Toyobo) using 1 lL of the plasmid RTG-2 cDNA library described above as the template. The PCR consisted of one initiation cycle of 2 min ... digoxigenin; HA, hemagglutinin; EMSA, electrophoretic mobility shift assay; EGS, ethylene glycol bis (succinimidyl succinate); DBD, DNA binding domain; HR, hydrophobic heptad repeat; NLS, n...

Ngày tải lên: 19/02/2014, 12:20

... catalysis; X-ray structure. Chitin, b-(1,4)-linked poly (N-acetylglucosamine), is one of the most abundant polymers in nature. It is a major component of the cell wall of yeast and other fungi, and ... Hevea brasiliensis. It is located in so-called lutoid bodies, which are low pH vacuolar organelles filled with hydrolytic enzymes and lectins [7]. These lutoid bodies are believed to...

Ngày tải lên: 22/02/2014, 04:20

... MLIII, respectively. Materials and methods Plant material Young leaves of mistletoe (Viscum album L) were harvested at the end of May from plants growing on a poplar tree (Populus alba L) in the Poltavskaya ... the MLp variants and ricin The MLp variants have a high overall sequence similarity to structurally and biologically related ricin D [26]. The A-chains of ML1p, ML2p a...

Ngày tải lên: 07/03/2014, 15:20

... N-terminus of the protein (Fig. 3). Cellular localization. The c ellular localization of myc-tag- D14-SR was examined in transiently t ransfected COS-7 cells. Double immuno¯uorescence analysis of cells ... Biochemical Sciences ÔA. Rossi FanelliÕ, Universita Á ÔLa SapienzaÕ Roma, Italy; 4 Department of Biochemical Sciences and Molecular Biotechnology, University of Perugia, Italy B...

Ngày tải lên: 08/03/2014, 16:20

... to homogeneity by HPLC and digested with t rypsin. The tryptic peptides were separated by HPLC and some of them were totally or partially analyzed by Edman sequencing and/ or by MALDI-TOF mass spectrometry ... protein of Brassica, spinach, barley and A. thaliana,PR1ofPhaseolus and MHF 9 of A. thaliana. These proteins b elong to the 2Cys-Prx subfamily. A ll plan t 2Cys-Prx prote...

Ngày tải lên: 08/03/2014, 16:20

... and 20% (v/v) glycerol and sonicated. COS7 cells (5 · 10 6 cells) were washed with NaCl/P i , trypsinized and collected in NaCl/P i (pH 7.2) containing 20% glycerol and 1 · PIC. Cells were homogenized ... carboxylase activity and vitamin K- dependent epoxidase activity. The recombinant carboxylase hasamolecularmassof 130 kDa. The recombinant Conus carboxylase carboxylated Phe-Leu-G...

Ngày tải lên: 17/03/2014, 10:20