Báo cáo y học: " Cloning and characterization of microRNAs from wheat (Triticum aestivum L )" docx
Báo cáo y học: " Cloning and characterization of microRNAs from wheat (Triticum aestivum L.)" docx
...
*
Key Laboratory of Crop Heterosis and Utilization (MOE) and State Key Laboratory for Agrobiotechnology, Key Laboratory of Crop
Genomics and Genetic Improvement (MOA), Beijing Key Laboratory of ... below
spikes, leaves, flag leaves, roots and spikes, were probed with labeled oligonucleotides. The tRNA and 5S RNA bands were visualized by ethidium bromide
staining of polya...
Báo cáo Y học: Cloning and characterization of novel snake venom proteins that block smooth muscle contraction pptx
... resulting from application of
calciseptine, a well-characterized neurotoxin from black
mamba (Dendroaspis polylepis polylepis).Calciseptineisa
known blocker of L- type Ca
2+
channels, a property that
underlies ... m
M
dithiothreitol in the presence of 0.5
M
Tris/HCl,
pH 8.5, 6
M
guanidine hydrochloride, and 2 m
M
EDTA in
a volume of 0.5 mL. Three microliters of 4-vinylpyridin...
Báo cáo Y học: Cloning and characterization of the mammalian-specific nicolin 1 gene (NICN1) encoding a nuclear 24 kDa protein doc
... Nuclear import of glycoconjugates is distinct
from the classical NLS pathway. J. Cell Sci. 108, 1325–1332.
SUPPLEMENTARY MATERIAL
The following material is available from http://www.
blackwell-science.com/products/journals/suppmat/EJB/
EJB3232/EJB3232sm.htm
Fig. ... was
added.
Biosynthetic labeling of transfected cells
and immunoprecipitation of cell extracts
COS-1 cells were cult...
Tài liệu Báo cáo khoa học: Cloning and characterization of CBL-CIPK signalling components from a legume (Pisum sativum) ppt
... by immunofluorescence labelling
and confocal microscopy
Localization of PsCIPK and PsCBL was analysed by
immunofluorescence labelling of tobacco BY2 cells fol-
lowed by confocal microscopy. Cell ... partially
cloned by PCR and then followed by isolation of full-length
clones by library screening. For partial cloning of pea CIPK
(PsCIPK) or pea CBL (PsCBL), all the known sequence...
Tài liệu Báo cáo khóa học: Cloning and characterization of two distinct isoforms of rainbow trout heat shock factor 1 ppt
... in a total volume of 50 lL with KOD-Plus-DNA
Polymerase (Toyobo) using 1 lL of the plasmid RTG-2
cDNA library described above as the template. The PCR
consisted of one initiation cycle of 2 min ... digoxigenin;
HA, hemagglutinin; EMSA, electrophoretic mobility shift assay;
EGS, ethylene glycol bis (succinimidyl succinate); DBD,
DNA binding domain; HR, hydrophobic heptad repeat;
NLS, n...
Tài liệu Báo cáo Y học: Expression and characterization of active site mutants of hevamine, a chitinase from the rubber tree Hevea brasiliensis docx
... catalysis; X-ray structure.
Chitin, b-(1,4)-linked poly (N-acetylglucosamine), is one of
the most abundant polymers in nature. It is a major
component of the cell wall of yeast and other fungi, and ... Hevea
brasiliensis. It is located in so-called lutoid bodies, which are
low pH vacuolar organelles filled with hydrolytic enzymes
and lectins [7]. These lutoid bodies are believed to...
Báo cáo khoa học: Cloning and characterization of the genes encoding toxic lectins in mistletoe (Viscum album L) pot
... MLIII,
respectively.
Materials and methods
Plant material
Young leaves of mistletoe (Viscum album L) were harvested
at the end of May from plants growing on a poplar tree
(Populus alba L) in the Poltavskaya ... the MLp
variants and ricin
The MLp variants have a high overall sequence similarity
to structurally and biologically related ricin D [26]. The
A-chains of ML1p, ML2p a...
Báo cáo Y học: Cloning and expression of sterol D14-reductase from bovine liver potx
... N-terminus of the protein (Fig. 3).
Cellular localization. The c ellular localization of myc-tag-
D14-SR was examined in transiently t ransfected COS-7
cells. Double immuno¯uorescence analysis of cells ... Biochemical Sciences ÔA. Rossi FanelliÕ, Universita
Á
ÔLa SapienzaÕ Roma, Italy;
4
Department of Biochemical Sciences and Molecular Biotechnology, University of Perugia, Italy
B...
Báo cáo Y học: Isolation and characterization of a thioredoxin-dependent peroxidase from Chlamydomonas reinhardtii doc
... to
homogeneity by HPLC and digested with t rypsin. The
tryptic peptides were separated by HPLC and some of them
were totally or partially analyzed by Edman sequencing
and/ or by MALDI-TOF mass spectrometry ... protein of Brassica, spinach, barley and
A. thaliana,PR1ofPhaseolus and MHF
9
of A. thaliana.
These proteins b elong to the 2Cys-Prx subfamily. A ll plan t
2Cys-Prx prote...
Báo cáo Y học: Expression and characterization of recombinant vitamin K-dependent c-glutamyl carboxylase from an invertebrate, Conus textile doc
... and 20%
(v/v) glycerol and sonicated. COS7 cells (5 · 10
6
cells) were
washed with NaCl/P
i
, trypsinized and collected in NaCl/P
i
(pH 7.2) containing 20% glycerol and 1 · PIC. Cells were
homogenized ... carboxylase activity and vitamin K-
dependent epoxidase activity. The recombinant carboxylase
hasamolecularmassof 130 kDa. The recombinant Conus
carboxylase carboxylated Phe-Leu-G...
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