Báo cáo y học: "The fungal frontier" potx
Báo cáo y học: "The fungal frontier" potx
... genome and annotation of Phycomyces blakesleeanus, a classic model for fungal sensory physiology. Only the second zygomycete to be sequenced, the genome sequence has already shed light on families ... (University of California, Berkeley, USA) reported on the results of phylogenetic analysis. The P450 gene family, whose members are critical for lignin breakdown, arose independently in Phaner...
Ngày tải lên: 14/08/2014, 07:21
Báo cáo y học: "The next frontier of systems biology: higher-order and interspecies interactions" pps
... strategy for studying biological systems than as a field of biology. Systems approaches have been very successful in the realms of biochemistry and genetics, especially for genetically tractable ... of most labs. Second, the costs of systems biology scale sub-linearly while the payoffs scale super-linearly. Put simply, screening 100 times as many genes yields more than 100 times the inf...
Ngày tải lên: 09/08/2014, 20:21
Tài liệu Báo cáo Y học: The insert within the catalytic domain of tripeptidyl-peptidase II is important for the formation of the active complex potx
... describe d in Materials a nd methods. Enzyme activity was a nalysed by the standard assay and the i mmunoreactivity was detected by Western blot analysis and quantitated as described i n Materials ... described previously usin g a ffinity purifi ed polyclonal chicken anti-(human TPP II) Ig [25]. The immunoreactivity was quantitated from scanned X-ray films by use of the MOLECULAR ANALYST softwar...
Ngày tải lên: 21/02/2014, 15:20
Báo cáo Y học: The Saccharomyces cerevisiae type 2A protein phosphatase Pph22p is biochemically different from mammalian PP2A potx
... of other polycations on phosphorylase phosphatase activity of yeast and mamma- lian PP2Ac poly- L -lysine was added to the purified enzymes. As presented in Fig. 5 a peak of poly- L -lysine- stimulated ... activity vs. 2.5-fold activation of PP2Ac). PR65/A subunit (3 n M ) increased activation of PP2Ac by poly- L -lysine by approximately 70% and it also increased stimulation of Pph22p by poly-...
Ngày tải lên: 08/03/2014, 22:20
Báo cáo Y học: The proximal cis-acting elements Sp1, Sp3 and E2F regulate mouse mer gene transcription in Sertoli cells potx
... Polyadenylated RNA was prepared by oligo(dT) affinity chromatography using the PolyATract System IV (Promega). Northern blot analysis Northern blot analysis was performed as described previ- ously [20]. ... of antibody and incubation on ice for 30 min. The reactions were separated by polyacrylamide gel elec- trophoresis (6%), and analysed by autoradiography. Site-directed mutation analysis Sit...
Ngày tải lên: 08/03/2014, 23:20
Báo cáo Y học: The role of arginine residues in substrate binding and catalysis by deacetoxycephalosporin C synthase potx
... deacetylcephalosporin C (DAC 3)is catalysed by a closely related oxygenase, deacetylcephalo- sporin C synthase (DACS; Swissprot P42220). The DAC 3 product is then converted into cephamycin C ... deacetylcephalosporin C synthase; DAOC, deacetoxycephalosporin C; DAOCS, deacetoxycephalosporin C synthase; Dnase 1, deoxyribonuclease 1; EDTA, ethylenediaminetetraacetic acid; ESI MS, electrospray .....
Ngày tải lên: 18/03/2014, 01:20
Báo cáo Y học: The human b-globin locus control region A center of attraction potx
... Nucleosome fractionation by mercury affinity chromatography: contrasting distribution of transcrip- tionally active and acetylated histones in nucleosome fractions of wild-type yeast cells and cells ... suggest th at dynamic changes in the acetylation patterns, initiated by the recruitment of h istone acetyltransferase a nd deacetyltransferase to the LCR, may affect globin gene expression by re...
Ngày tải lên: 18/03/2014, 01:20
Báo cáo Y học: The presence of phosphate at a catalytic site suppresses the formation of the MgADP-inhibited form of F1-ATPase potx
... thermophilic F 1 -ATPase activity. ATP hydrolysis assay ATP hydrolysis was measured using an ATP-regenerating system as a decrease in A 340 of NADH at 25 °C. The assay mixture contained 50 m M Mops/KOH ... have previously reported th at the DNC a 3 b 3 c sub com - plex did not exhibit steady-state ATP hydrolysis in the absence o f P i [16], but signi®cant steady-state ATP hydro- lysis was lat...
Ngày tải lên: 24/03/2014, 00:21
Báo cáo Y học: The sodium pump Its molecular properties and mechanics of ion transport potx
... importance of Lys480 for ATP hydrolysis and enzyme function [27]. Various other ATP analogs such as 5¢-p-fluoro- sulfonylbenzoyl-adenosine (FSBA) or c-[4-(N-2-chloro- ethyl-N-methylamino)]benzylamide ... Lys480 by 8-azido-ATP [23] does not necessarily indicate that this amino acid directly interacts with the adenine moiety of the ATP molecule, but that it is merely within reach of the highly ....
Ngày tải lên: 24/03/2014, 00:21
Báo cáo Y học: The porcine trophoblastic interferon-c, secreted by a polarized epithelium, has specific structural and biochemical properties potx
... N-term. pyroglut. 7 12 EITILK 716.455 716.452 )0.003 13 34 DYF…ILK 2397.198 4540.260 4394.224 4250.802 2143.062 1997.026 1853.604 Glycosylation Glycosylation Glycosylation 44 55 IIQSQIVSFYFK 1472.814 ... apomyoglobin (M + H) + ¼ 16 952.6 Da and bovine carbonic anhydrase (M + H) + ¼ 29 024 Da. Tryptic peptide analysis by MALDI-TOF. Tryptic diges- tions of glycosylated IFNs were achieved direct...
Ngày tải lên: 24/03/2014, 04:21