Báo cáo y học: "Isolation and identification of bioactive compounds in Andrographis paniculata (Chuanxinlian)" potx

... aerial part of A. paniculata is commonly used in Chinese medicine. According to Chinese medicine theory, A. paniculata 'cools' and relieves internal heat, inflammation and pain and is used ... effects in treating acute upper respira- tory tract infections and relieving the inflammatory symptoms of sinusitis [85]. The migratory response of leukocytes to chemokin...

Ngày tải lên: 13/08/2014, 15:21

... proteins [8,23] and to identify in vitro the most reactive internal cysteine (Cys207) of Sorghum NADP-MDH [12]. The cytosolic Trx h of Chlamydomonas has only two cysteines in its primary sequence, ... 2Cys-Prx subgroup, and 1Cys-Prx subgroup contain two and one conserved cysteines, respectively. 2Cys-Prx proteins are reduced by the AhpF protein in bacteria, and by the thiore...

Ngày tải lên: 08/03/2014, 16:20

... FEBS 2002 mass ranging from 95 to over 100 kDa in polyacrylamide gels. Antibody staining of sections from bovine prelactating and lactating mammary gland using monoclonal and polyclonal antibodies ... 2-propanol were collected and freeze- dried. Standard procedures were employed analysing pro- tein samples by SDS/PAGE using 18% polyacrylamide gels, and for the staining of protei...

Ngày tải lên: 24/03/2014, 04:21

... 72.7 Ofloxacin 00 Cefixime 00 Polymyxin B 0 36.4 Cefotaxime 0 18.2 Sparfloxacin 00 Cephalothin 18.2 27.3 Streptomycin 0 36.4 Chloramphenicol 00 Tetracycline 18.2 36.4 Ciprofloxacin 00 Tobramycin 00 Erythromycin 100 0 Trimethoprim/ sulfamethoxazole 00 Gentamicin 00 Imipenem 00 Molecular ... followed by EtBr staining and photographed under UV illumination. RAPD fingerprinting To increase the...

Ngày tải lên: 07/08/2014, 18:21

... the clinical Isolation and identification of a canine coronavirus strain from giant pandas 263 Fig. 3. Analysis of GPV based on gene sequencing. (A) Comparison of amino acid sequences of S gene ... indicates the cytoblas t of an infected FCWF cell. The black arrow indicates the inclusion bodies of GPV in the cytoplasm of cells. Scale bars = 200 nm. 10 volumes of Han...

Ngày tải lên: 07/08/2014, 23:22

... proteins. By in creasing the temperature of the heat treatment to 70°C, band X faded, whereas the intensity of band Y increased. With further increases in temperature, band Y faded, whereas the intensity ... glycine, and 25 mM Tris. N-terminal amino acid sequence analysis and amino acid sequence homology search After SDS-PAGE, proteins in the polyacrylamide gels were electro...

Ngày tải lên: 12/08/2014, 01:21

... lymphocytes) following chemical (cytokines, thrombin and endotoxin), physical (shear stress and hypoxia) [17] and apoptotic [18] stimuli. One of the first described roles for MPs was in the initiation ... from 50%, 70%, 95% and 100% for 10 minutes in each solution. The sample was finally dehydrated in pro- pylene oxide for 15 minutes. Finally, the sample was embedded in epox...

Ngày tải lên: 12/08/2014, 11:22

... University of Minnesota, Minneapolis, Minnesota, USA and 2 Dept. of Genetics, Cell Biology and Development and the Institute of Human Genetics, University of Minnesota, Minneapolis, Minnesota, ... efficiency was determined at the hypoxan- thine guanine phosphoribosyl transferase (HPRT) locus and at the adenine phosphoribosyltransferase locus by plating in medium containing...

Ngày tải lên: 13/08/2014, 05:22

... as those for hypothetical proteins, expressed proteins and putative proteins, are not included in the target prediction because of the difficulty in identifying their orthologs in the O. sativa ... miR- NAs bind to the protein-coding region of their mRNA target with nearly perfect sequence complementarity, and degrade the target mRNA in a way similar to RNA interference (RNAi)...

Ngày tải lên: 14/08/2014, 14:21

... 2002 D14-SR. To verify our hypothesis, bovine cDNA encoding the 38-kDa protein was cloned to iden tify the catalytic activity of the expressed protein. Cloning of the cDNA encoding bovine sterol D14-reductase Bovine ... DISCUSSION Isolation of sterol D14-reductase During the preparation and delipidation of a bovine liver 38-kDa protein exhibiting EPT activity [25], a protein co- mig...

Ngày tải lên: 08/03/2014, 16:20