Báo cáo y học: " The establishment and characterization of the first canine hepatocellular carcinoma cell line, which resembles human oncogenic expression patterns" ppsx

... Division of Pathology, Faculty of Veterinary Medicine, Utrecht University, The Netherlands and 3 Department of Infectious Diseases and Immunology, Division of Virology, Faculty of Veterinary Medicine, ... with the neoplastic nature of the cells [11], and suggests the presence of both fully differentiated hepato- cytes and progenitor cells in the cHCC cell c...

Ngày tải lên: 13/08/2014, 13:20

... composition of the cell wall, DNA organization, synthesis of storage compounds such as glycogen and polyphosphate, and other cellular processes [1,2]. As a result of these changes, the cells become ... m M phenylmethanesulfonyl fluoride. Cells were then disrupted by treatment with lysozyme (1 mgÆmL )1 )onice for 10 min followed by a single rapid cycle of freeze and thaw at...

Ngày tải lên: 22/02/2014, 07:20

... allowed removal of theophylline, and a reduction of volume and detergent concentration. The higher degree of re ceptor homogeneity obtained by the ion- exchange step will help crystallization. ... determination of hA2aR by X-ray or electron crystallography, or infor- mation on the bound ligand conformation obtained by NMR spectroscopy, would assist in the design of subty...

Ngày tải lên: 24/03/2014, 00:21

... activity of the TRH-DE released into the culture medium, suggesting that the increase in the enzymatic activity is due to the increased synthetic a ctivity of hepatocytes and not due to cell leakage. For ... enzyme, the serum e nzyme and th e liver enzyme strongly suggeste d that these proteins differ only in their carbohydrate moiety. Analysis of the carbohydrate st...

Ngày tải lên: 31/03/2014, 21:21

... rearrangements. In the thyroid, endocytosis of thyroglobulin is the first step of the thyrotropin stimulated secretion of thyroid hormones. In the elucidation of the cellular function of the small G proteins, ... transcription/translation kit TnT (Promega, Leiden, the Netherlands) under the control of T7 promoter using pcDNA3.HIS, and the quality of synthesis...

Ngày tải lên: 23/03/2014, 21:20

... entry of free viruses, and the maturation or egress of the virus [8,9]. Glycoprotein K is one of the major glycoproteins encoded by the DEV-gK gene, which is located in the unique long region of ... is the first time to product and purify the rabbit anti-tgK polyclonal antibody. Through the western blot and ELISA assay, the truncated glycoprotein K (tgK) ha...

Ngày tải lên: 12/08/2014, 01:21

... Th2, Th17 and T reg responses may exist in fish, and arose early in verte- brate evolution, has been prompted by the discovery of many of the cytokines that are expressed by these cell types [22,23]. ... variety of tissues and cells in the Ginbuna crucian carp [27] and, in mammals, in the lung tissue of mouse and spleen and thymus of human and mouse [11,37]....

Ngày tải lên: 16/02/2014, 09:20

... A ˚ ke Engstro ¨ m at the Peptide Synthesis and Analysis Laboratory of the Department of Medical Biochemistry and Microbio- logy, Uppsala University. The SP6 primer 5¢-ATT TAG GTG ACA CTA TAG-3¢ and the three ... other combinations of phosphatases and interme- diary phosphorylated enzymes. Therefore, kinetic data on the dephosphorylation of autophosphorylated ATP-cit...

Ngày tải lên: 21/02/2014, 01:21

... membrane and in addition to de novo synthesis, the plant enzyme is capable of catalysing the exchange of the inositol polar head for another inositol. Our data clearly show that the CDP-diacylglycerol-independent exchange ... allow detectable synthesis. The level of PtdIns in the membranes does not allow synthesis of detectable PtdIns by exchange of the polar head. A...

Ngày tải lên: 22/02/2014, 04:20

... family Caricaceae [1]. The two economically most important genera of this family are the commonly grown tropical species Carica papaya and the group of highland papa- yas (Vasconcellea spp.), of which ... equivalent or slightly higher proteolytic and lipolytic activity than that of papaya [22]. In a larger study, comparing the proteolytic activity of C. papaya latex wi...

Ngày tải lên: 07/03/2014, 11:20