... temperature in the same facility.Virgin flies from each of the seven genotypes were collectedand housed in single sex vials in groups of ten for three days.On the morning of the fourth day, 3 replicates ... introns,levels of polymorphism in the first intron and the combineddata for all introns were examined separately. Divergencealong the D. simulans lineage was also calculated for each of these features.Association ... 6.865). This analysis, however, may be con-founded by the fact that most genes are not statistically signif-icantly different among lines and that inclusion of these genes in the analysis may obscure...