Báo cáo y học: " Rev-dependent lentiviral expression vector" pdf

... of lymphomas h1 and h2 were analyzed by dot blot hybridization with [ 32 P]-labeled cDNA populations from SIV-associated monkey lymphomas and monkey B-lymphocytes. Those cDNAs whose hybridization ... markedly stronger with lymphoma cDNA than with cDNA of B- lymphocytes were further analyzed by Northern blot hybridization. Some genes (a-myb, set, SMG-1, ND4) involved in HIV-associated l...

Ngày tải lên: 02/11/2012, 11:08

... by 12.5 % sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Proteins were transferred to polyvinyl difluoride (PVDF) membrane (Bio- Rad, Hercules CA) and images were analyzed ... shown any changes by gene array (Table 1). Table 1. Analysis of IAPs expression in the HepG2.215 and HepG2 cells One hundred micrograms RNA from HepG2.215 and HepG2 cells were analyzed by g...

Ngày tải lên: 02/11/2012, 11:17

... cells were examined by ¯uorescence microscopy and the images were acquired by using a Spot-2 cooled camera (Diagnostic Instruments). Sterol D14-reductase assay D14-SR activity was assayed in microsomes ... family [37] were present at positions 12±18 and 337±348 of the deduced amino-acid sequence, respec- tively. Ergosterol b iosynthesis ERG4/ERG24 f amily signa- tures, Gx(2)[LIVM][YH]Dx[FYV]x...

Ngày tải lên: 08/03/2014, 16:20

... Biology (ZMBP), University of Tu ¨ bingen, Germany; 2 Department of Plant Biology, University of Valencia, Spain The aldo-keto reductase (AKR) superfamily comprises proteins that catalyse mainly ... reaction, are catalysed by enzymes of the aldo-keto reductase (AKR) superfamily [8]. The members of the AKR superfamily are cytosolic, monomeric proteins that catalyse mainly the NAD(P)H- dependen...

Ngày tải lên: 18/03/2014, 01:20

... RPMI- . Lymphocytes were counted using a hemocy- tometer chamber (Superior, Germany), and viability was de- termined by trypan blue exclusion. Antibodies and Flow Cytometric Analysis Antibodies ... host im- munity, possibly by upregulating the expression of CD on vaginal and peripheral T cells. Furthermore, resolution of the infection may depend on the ability of T lymphocytes to c...

Ngày tải lên: 08/08/2014, 21:20

... restricted to activated T lympho- cytes [5,22,23] and could be observed in resting T lym- phocytes, B lymphocytes, monocytes or granulocytes [20,24]. In contrast, Th2 lymphocytes were reported to produce ... osteoarthritis (OA) were analyzed using Affymetrix HuGeneFL microarrays. Data assessment was done using Affymetrix Microarray Suite 5.0. CXCL, Cys–X–Cys ligand; CXCR, Cys–X–Cys receptor;...

Ngày tải lên: 09/08/2014, 01:23

... analyzed individually. Flow cytometry analysis The cell type composition and level of protein expression for some genes in the inguinal lymph nodes were analyzed by flow cytometry. Single cell suspensions ... USA) and kindly donated by Dr Torres-Nagel (Institute for Virol- ogy and Immunobiology, University of Wurzburg, Germany). The lymph nodes were analyzed for the total numbers of leucocy...

Ngày tải lên: 09/08/2014, 01:23

... members of the TNF family were represented on the cDNA arrays, the expression of genes for only these two cytokines was modulated by protein I/II. IL-6 and leukemia inhibitory factor, which both ... leukemia inhibitory factor by rheu- matoid synovium and synoviocytes. Eur J Immunol 1994, 24:3222-3228. 23. Arnett FC, Edworthy SM, Bloch DA, McShane DJ, Fries JF, Cooper NS, Healey LA, Kaplan S...

Ngày tải lên: 09/08/2014, 06:22

... distributions. Figure 8 Flow cytometry profile of CXCR3 expression in peripheral blood (PB) and synovial fluid (SF) lymphocytes from patient 3 and a control subjectFlow cytometry profile of CXCR3 expression in ... that CXCR3 is expressed at high density by virtually all T lymphocytes isolated from synovial fluid (SF) and infiltrating the synovial membrane. Particularly strongly stained CX...

Ngày tải lên: 09/08/2014, 06:22

... analyzed for BMP expression by using microarray hybridization. Differential expression of BMP-4 and BMP-5 was validated by semiquantitative RT-PCR, in situ hybridization and immunohistochemistry. ... joint physiology and pathology. Introduction In patients with rheumatoid arthritis (RA), joint pathology is mediated by typical changes in the synovial tissue. Hyperpla- sia of the synovial l...

Ngày tải lên: 09/08/2014, 07:20