Báo cáo y học: " Phosphorylation of HIV-1 Tat by CDK2 in HIV-1 transcription" ppt

... purposes) PKR phosphorylation of HIV-1 Tat wild-type and mutants after 15 minutes and phosphorylation kineticsFigure 5 PKR phosphorylation of HIV-1 Tat wild-type and mutants after 15 minutes and phosphorylation ... the antiviral effects of IFN by the direct binding of its Tat protein to PKR [21]. In the infected cell, Tat blocks the inhibition of protein synt...

Ngày tải lên: 18/06/2014, 22:20

... containing HIS-tagged Tat pro- teins; M lanes, 14 kDa marker; C lanes, BL21(DE3)pLysS cell extract. M C Tat Tat Tat M C Tat Tat Tat Tat M C Tat Tat Tat M C Tat Tat M C Tat Tat Tat M C Tat Tat Tat ... the activity of the enzyme is blocked by Tat and Tat itself is phosphorylated by PKR [21] at serine 62, threonine 64 and serine 68 [22]. HIV-1 Tat is a 14 k...

Ngày tải lên: 20/06/2014, 04:20

... block intracellular enzymes that control signaling pathways. Inhibitors of tyrosine kinases expressed in lymphocytes, such as spleen tyrosine kinase and Janus kinase, are being tested in autoimmune ... Class I PI3Ks are a family of intracellular signaling proteins involved in many aspects of cell biology, including adaptive and innate immunity [65]. They are composed of heterodi...

Ngày tải lên: 09/08/2014, 01:22

... 1 Analysis of HIV-1 Tat phosphorylated by CDK2/ cyclin E in vitro. A, Tat is phosphorylated by CDK2. Recombinant Tat was phosphorylated in vitro by purified CDK2/ cyclin E (lane 1), by HeLa nuclear ... phosphorylated by CDK2/ cyc- lin E in vitro [20]. This dynamic interaction greatly stimu- lated the activity of CDK2/ cyclin E toward phosphorylation of CTD...

Ngày tải lên: 13/08/2014, 09:20

... mediated by in uencing its phosphorylation status and/or its interac- tion with the eIF4E binding proteins, as well as by affecting the integrity of eIF4G which serves as an adapter protein that ... GTP. As GTP binding to eIF2 is a prerequisite to Met-tRNA i binding, phosphorylation of eIF2a effectively inhibits eIF2 recycling and consequently inhibits additional translation init...

Ngày tải lên: 22/02/2014, 07:20

... During HIV-1 infection, the Tat protein plays a key role by transactivating the transcription of the HIV-1 proviral DNA. In addition, Tat induces apoptosis of non-infected T lymphocytes, leading ... Since the highly conserved cysteine-rich domain of Tat [22,23] likely induces a structure distinct from the eukaryotic zinc fingers, interference with zinc binding to Tat or...

Ngày tải lên: 13/08/2014, 05:21

... were observed in response to p-synephrine or p-synephrine in combination with naringin and hes- peridin (Table 2). p-Synephrine differs from ephedrine in that it has a hydroxyl group on the ... self-report ratings of symp- toms increases confidence in the safety of the combi- nation as well as in the blinding procedure used in this study. Strengths of the study are the u...

Ngày tải lên: 25/10/2012, 11:04

... indicate the difficulty in amplifying the sequences in these re- gions. In our study, we attempted to amplify each ex- tremity, but failed. Interestingly, phylogenetic analysis based on the NS1 ... etiology of hepatitis remains obscure in 3-10% of cases in Europe [5] and the United States [6] and up to 30% of cases in Asia [7, 8]. Particularly, the cause of fulminant...

Ngày tải lên: 26/10/2012, 10:03

... detected by silver staining on polyacrylamid gel. Statistical Analysis Allele frequencies were determined by gene counting and compared using the א² test. The data was analysed using SPSS 12.0 software. ... characterized by a rise of HDL and lowering of LDL, triglycerides, and insulin [5]. In addition, transgenic expression of an activated PPARδ in mice conferred resistan...

Ngày tải lên: 31/10/2012, 17:03

... recently, methods were devised for predicting residues involved in protein interaction s ites in the absence of any structural reports. By analysing hydrophobicity distribution, linear stretches of ... some of the complexes or by other experimental means. CONCLUSIONS We have analysed the possibility of predicting the residues forming part of protein–protein interacting surfa...

Ngày tải lên: 21/02/2014, 15:20