ENZYME KINETICS A MODERN APPROACH – PART 8 ppt
ENZYME KINETICS A MODERN APPROACH – PART 8 ppt
... Groutas et al., 19 98; Macchia et al., 2000; Clemente et al., 2001). Amino acid analysis of both native and inactivated enzyme can identify which amino acid is modified (for examples see Pochet et al., ... K i of an alter- nate substrate. Addition of an alternate substrate inhibitor to an enzyme assay results in an exponential decrease in rate to some final steady- state turnover of substra...
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ENZYME KINETICS A MODERN APPROACH – PART 2 pptx
... TOOLS AND TECHNIQUES OF KINETIC ANALYSIS 1.4 ACID–BASE CHEMICAL CATALYSIS Many homogeneous reactions in solution are catalyzed by acids and bases. ABr ¨ onsted acid is a proton donor, HA + H 2 O ... for example, the reaction A → B → C. Usually, equations in differential or algebraic form are fitted to indi- vidual data sets, A, B, and C and a set of parameter estimates obtained. 26 TOOL...
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ENZYME KINETICS A MODERN APPROACH – PART 4 pptx
... is appropriate. When more than two values are compared, a one-way analysis of variance (ANOVA), TABLE 4.2 Rate of Hydration of Fumarate to Malate by Fumarase at various Substrate Concentrations a Velocity ... K ∗ s correspond, respectively, to apparent enzyme maxi- mum velocity and apparent enzyme substrate dissociation constant at a particular pH. For the model above, V ∗ max = V ma...
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ENZYME KINETICS A MODERN APPROACH – PART 5 pptx
... cooperativity, which can have noninteger values. Estimates of the parameters k and n are obtained using standard non- linear regression procedures available in most modern graphical software packages. ... constant, Eq. (7.11) can be expressed as v V max = [B] K + [B] (7.12) where V max = V max [A] K BA + [A] (7.13) and K = K A s K AB + K AB [A] K BA + [A] = K AB (K A s +...
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ENZYME KINETICS A MODERN APPROACH – PART 6 pptx
... exponential term, and the shape of the curve approaches that of a straight line. Valuable information can be gained from analysis of the early and late stages of this reaction. 11.2.1 Early Stages ... plot for an interfa- cial enzyme. various parameters in Eq. (10.9) on the velocity of a reaction catalyzed by an interfacial enzyme. As the enzyme interface dissociation constant increas...
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ENZYME KINETICS A MODERN APPROACH – PART 1 pps
... expressed as d [A] dt =−k r [A] (1 .8) Integration of Eq. (1 .8) for the boundary conditions A = A 0 at t = 0and A = A t at time t, A t A 0 d [A] [A] =−k r t 0 dt(1.9) yields the integrated rate equation ... specifically disclaim any implied warranties of merchantability or fitness for a particular purpose. No warranty may be created or extended by sales representatives...
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ENZYME KINETICS A MODERN APPROACH – PART 3 pps
... statistic: χ 2 = n i=1 (y i −ˆy i ) 2 s 2 i = n i=1 w i (y i −ˆy i ) 2 (1.125) Consider a typical experiment where the value of a dependent variable is measured several times at a particular value of the independent variable. From these repeated determinations, a mean and variance ... Exact Analytical Solution (Non-Steady-State Approximation) Exact analytical solutions for the rea...
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ENZYME KINETICS A MODERN APPROACH – PART 7 ppsx
... treatment above assumes that there are no differences in heat capac- ity between native and denatured states of an enzyme and that the heat capacity remains constant throughout the temperature ... stability, enzyme solutions are incubated at a particular temperature and aliquots removed at the appro- priate times. Enzyme activity in these samples is then measured at the enzyme s temp...
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ENZYME KINETICS A MODERN APPROACH – PART 9 docx
... less than either valine and serine mutants, which had comparable catalytic constants. Compared to valine and serine, alanine has the smallest van der Waals volume and accessible surface area. Therefore, ... mean of a minimum of two determi- nations ± standard deviation. b ss1, substrate consisting of the peptide lysine–proline–alanine–glutamic acid–phenylalanine– phenylalanine (NO 2 )–alani...
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ENZYME KINETICS A MODERN APPROACH – PART 10 pps
... entries acid–base, 2 0–2 3 enzyme, 4 1–4 3, 4 8 5 2, 5 2–5 3 in mechanism-based inhibition, 15 8 1 59 models of, 4 8 5 2 pH dependence of, 7 9 8 9 practical example of enzyme, 5 3–5 8 Catalysts, enzymes as, 4 1–4 3 Catalytic ... enzyme- catalyzed reactions, 7 9 8 2 plotting substrate concentration versus, 5 0–5 2, 5 3–5 8, 10 3–1 09, 113, 11 4–1 15, 1 78, 17 9–1 81...
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