ENZYME KINETICS A MODERN APPROACH – PART 4 pptx
ENZYME KINETICS A MODERN APPROACH – PART 4 pptx
... is appropriate. When more than two values are compared, a one-way analysis of variance (ANOVA), TABLE 4. 2 Rate of Hydration of Fumarate to Malate by Fumarase at various Substrate Concentrations a Velocity ... phenomenological irreversible inhibition mechanisms are discussed in turn. APPLICATIONS 67 TABLE 4. 3 Estimates of the Catalytic Parameters for the Fumarase-Catalyzed hydration of...
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ENZYME KINETICS A MODERN APPROACH – PART 2 pptx
... TOOLS AND TECHNIQUES OF KINETIC ANALYSIS 1 .4 ACID–BASE CHEMICAL CATALYSIS Many homogeneous reactions in solution are catalyzed by acids and bases. ABr ¨ onsted acid is a proton donor, HA + H 2 O ... for example, the reaction A → B → C. Usually, equations in differential or algebraic form are fitted to indi- vidual data sets, A, B, and C and a set of parameter estimates obtained. 26 TO...
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ENZYME KINETICS A MODERN APPROACH – PART 5 pptx
... ternary enzyme substrate complexes EAB (K AB and K BA ), and the enzyme mass balance are, respectively, v = k cat [EAB] (7.7) K A s = [E] [A] [EA] K B s = [E][B] [EB] (7.8) K BA = [EB] [A] [EBA] K AB = [EA][B] [EAB] [E T ] ... constant, Eq. (7.11) can be expressed as v V max = [B] K + [B] (7.12) where V max = V max [A] K BA + [A] (7.13) and K = K A s K AB + K AB [A] K BA...
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ENZYME KINETICS A MODERN APPROACH – PART 6 pptx
... Ax becomes greater than that of the exponential term, and the shape of the curve approaches that of a straight line. Valuable information can be gained from analysis of the early and late stages of ... plot for an interfa- cial enzyme. various parameters in Eq. (10.9) on the velocity of a reaction catalyzed by an interfacial enzyme. As the enzyme interface dissociation constant increa...
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ENZYME KINETICS A MODERN APPROACH – PART 1 pps
... service, call 1-800-CALL-WILEY. Library of Congress Cataloging-in-Publication Data: Marangoni, Alejandro G., 1965- Enzyme kinetics : a modern approach / Alejandro G. Marangoni. p. ; cm. ISBN 0 -47 1-15985-9 ... 28 1.6.1.2 Least-Squares Minimization (Regression Analysis) / 29 1.6.2 Exact Analytical Solution (Non-Steady-State Approximation) / 39 1.6.3 Exact Analytical Solution (Steady...
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ENZYME KINETICS A MODERN APPROACH – PART 3 pps
... statistic: χ 2 = n i=1 (y i −ˆy i ) 2 s 2 i = n i=1 w i (y i −ˆy i ) 2 (1.125) Consider a typical experiment where the value of a dependent variable is measured several times at a particular value of the independent variable. From these repeated determinations, a mean and variance ... Exact Analytical Solution (Non-Steady-State Approximation) Exact analytical solutions for the rea...
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ENZYME KINETICS A MODERN APPROACH – PART 7 ppsx
... treatment above assumes that there are no differences in heat capac- ity between native and denatured states of an enzyme and that the heat capacity remains constant throughout the temperature ... stability, enzyme solutions are incubated at a particular temperature and aliquots removed at the appro- priate times. Enzyme activity in these samples is then measured at the enzyme s temp...
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ENZYME KINETICS A MODERN APPROACH – PART 8 ppt
... Loughran, and M. D. Walkinshaw, J. Biol. Chem. 2 74, 249 0 1–2 49 05 (1999). Vilain, A C., V. Okochi, I. Vergely, M. Reboud-Raveax, J P. Mazalelyrat, and M. Wakselman, Biochim. Biophys. Acta 1076, 40 1 4 05 ... Finer-Moore, Y. Wataya, and D. V. Santi, Biochemistry 33, 1508 6–1 50 94 (19 94) . Groutas, W. C., R. Kuang, R. Venkataraman, J. B. Epp, S. Ruan, and O. Prakash, Biochemistry...
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ENZYME KINETICS A MODERN APPROACH – PART 9 docx
... less than either valine and serine mutants, which had comparable catalytic constants. Compared to valine and serine, alanine has the smallest van der Waals volume and accessible surface area. Therefore, ... Wild-Type, Lys36pArg, and Lys36pMet Trx-PG at 14 ◦ C determined with the Synthetic Peptide Substrate Lysine–Proline–Alanine– Glutamic Acid– Phenylalanine–Phenylalanine (NO 2 )–Alanine–Leu...
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ENZYME KINETICS A MODERN APPROACH – PART 10 pps
... entries acid–base, 2 0–2 3 enzyme, 4 1 4 3, 4 8–5 2, 5 2–5 3 in mechanism-based inhibition, 15 8–1 59 models of, 4 8–5 2 pH dependence of, 7 9–8 9 practical example of enzyme, 5 3–5 8 Catalysts, enzymes as, 4 1 4 3 Catalytic ... enzymes, 14 0–1 57, 20 5–2 12 Standard-state enthalpy of denaturation, 14 7–1 50, 155 Standard-state entropy of denaturation, 14 7...
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