ENZYME KINETICS A MODERN APPROACH – PART 3 pps
ENZYME KINETICS A MODERN APPROACH – PART 3 pps
... not be assumed for the derivation of an enzyme catalysis model. A steady-state approximation can also be used to obtain the rate equation for an enzyme- catalyzed reaction. 3. 2.2 Steady-State Model The ... statistic: χ 2 = n i=1 (y i −ˆy i ) 2 s 2 i = n i=1 w i (y i −ˆy i ) 2 (1.125) Consider a typical experiment where the value of a dependent variable is measured several times...
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ENZYME KINETICS A MODERN APPROACH – PART 1 pps
... customer service, call 1-800-CALL-WILEY. Library of Congress Cataloging-in-Publication Data: Marangoni, Alejandro G., 1965- Enzyme kinetics : a modern approach / Alejandro G. Marangoni. p. ; cm. ISBN ... Integration / 28 1.6.1.2 Least-Squares Minimization (Regression Analysis) / 29 1.6.2 Exact Analytical Solution (Non-Steady-State Approximation) / 39 1.6 .3 Exact Analytical Solut...
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ENZYME KINETICS A MODERN APPROACH – PART 7 ppsx
... G ◦ D (T ) T (12 .32 ) The treatment above assumes that there are no differences in heat capac- ity between native and denatured states of an enzyme and that the heat capacity remains constant throughout ... stability, enzyme solutions are incubated at a particular temperature and aliquots removed at the appro- priate times. Enzyme activity in these samples is then measured at the e...
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ENZYME KINETICS A MODERN APPROACH – PART 10 pps
... entries acid–base, 2 0–2 3 enzyme, 4 1–4 3, 4 8–5 2, 5 2–5 3 in mechanism-based inhibition, 15 8–1 59 models of, 4 8–5 2 pH dependence of, 7 9–8 9 practical example of enzyme, 5 3 5 8 Catalysts, enzymes as, 4 1–4 3 Catalytic ... INDEX Abbreviations, table for mutation research, 21 4–2 16 Absolute reaction rate theory, 2 3 2 6 Acid–base catalysis, 2 0–2 3 Acidity...
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ENZYME KINETICS A MODERN APPROACH – PART 2 pptx
... constant, and only k 2 and k 3 floated. After preliminary parameter estimates are obtained in this fashion, these parameters should be fixed as constants and the remaining parameters estimated. ... complex reac- tion schemes. These are (1) numerical integration of differential equations, (2) steady-state approximations to solve differential equations analytically, and (3) exact analytical sol...
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ENZYME KINETICS A MODERN APPROACH – PART 4 pptx
... the comparison of two values, a two-tailed t -test is appropriate. When more than two values are compared, a one-way analysis of variance (ANOVA), TABLE 4.2 Rate of Hydration of Fumarate to Malate ... V ∗ max and K ∗ s correspond, respectively, to apparent enzyme maxi- mum velocity and apparent enzyme substrate dissociation constant at a particular pH. For the model above, V ∗ max =...
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ENZYME KINETICS A MODERN APPROACH – PART 5 pptx
... as v V max = [B] K + [B] (7.12) where V max = V max [A] K BA + [A] (7. 13) and K = K A s K AB + K AB [A] K BA + [A] = K AB (K A s + [A] ) K BA + [A] (7.14) From determinations of K and V max at ... cooperativity, which can have noninteger values. Estimates of the parameters k and n are obtained using standard non- linear regression procedures available in most moder...
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ENZYME KINETICS A MODERN APPROACH – PART 6 pptx
... exponential term, and the shape of the curve approaches that of a straight line. Valuable information can be gained from analysis of the early and late stages of this reaction. 11.2.1 Early Stages ... plot for an interfa- cial enzyme. various parameters in Eq. (10.9) on the velocity of a reaction catalyzed by an interfacial enzyme. As the enzyme interface dissociation constant increas...
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ENZYME KINETICS A MODERN APPROACH – PART 8 ppt
... K i of an alter- nate substrate. Addition of an alternate substrate inhibitor to an enzyme assay results in an exponential decrease in rate to some final steady- state turnover of substrate (Fig. 13. 1). ... Finer-Moore, Y. Wataya, and D. V. Santi, Biochemistry 33 , 1508 6–1 5094 (1994). Groutas, W. C., R. Kuang, R. Venkataraman, J. B. Epp, S. Ruan, and O. Prakash, Biochemistry 36 , 4 73...
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ENZYME KINETICS A MODERN APPROACH – PART 9 docx
... than either valine and serine mutants, which had comparable catalytic constants. Compared to valine and serine, alanine has the smallest van der Waals volume and accessible surface area. Therefore, ... mean of a minimum of two deter- minations ± standard deviation. b ss1, substrate consisting of the peptide lysine–proline–alanine–glutamic acid–phenylalanine– phenylalanine (NO 2 )–alanine–le...
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