Báo cáo y học: "Multiple independent origins of a protease inhibitor resistance mutation in salvage therapy patients" docx
... amplicons DNA is shown TCAGATTGGTTGCACTTT T A C C AGTCTAACCAACGTGAAA ATG AC TCAGATTGGTTGCACTTT TTG T A C C AGTCTAACCAACGTGAAA AC L90M specific primer binding to L90M L90M specific primer binding to ... drug resistance variation in vivo [4]. HIV protease inhibitors impair the maturation and result- ing infectivity of viral particles leading to a rapid decline in plasma viremia...
Ngày tải lên: 13/08/2014, 06:20
... are likely to play a significant role in nucleotide-induced pul- monary vasoconstriction as ATP, UTP and UDP each induce contractions of the rat pulmonary artery with matching pharmacological profiles. Competing ... was measured for 1 min immediately before and after antagonist addition and average values calculated. The % decrease in amplitude was then calculated as the difference...
Ngày tải lên: 12/08/2014, 18:20
... performed using lactacystin (Sigma), epoxomicin (Sigma) and MG132 (Sigma). The effect of the proteasome inhibitors was determined by analysing the capability of the treated cells to release the fluorogenic ... MJ: A clathrin, caveolae, and dynamin- independent endocytic pathway requiring free membrane cholesterol drives HIV-1 internalization and infection in polarized trophoblastic...
Ngày tải lên: 12/08/2014, 23:21
Báo cáo y học: "CD4-independent use of the CCR5 receptor by sequential primary SIVsm isolates" docx
... T cells, in chronically infected human T lymphocytes [44]and as well as in primary macrophages [32]. Viruses accumulated intracellularly in macrophages can retain infectivity for at least 6 weeks ... to late virus populations. Interestingly, in the P/SP group of macaques there was also a fraction of ani- mals that late in infection had a CD4 -independent- HIGH and neutraliza...
Ngày tải lên: 13/08/2014, 05:22
Báo cáo y học: "Multiple-center evaluation of mortality associated with acute kidney injury in critically ill patients: a competing risks analysis" pdf
... not modify patients’ management in any way, and because statistical analyses were processed anonymously, informed consent for participation in the study was waived. Study population and definitions All ... class were based on c 2 tests for categorical data and on one-way analysis of variance or the Kruskal-Wallis test for continuous data as appropriate. The association of AKI with mor...
Ngày tải lên: 14/08/2014, 08:21
Báo cáo y học: "Multiple independent evolutionary solutions to core histone gene regulation" potx
... sequences and, accordingly, the location/presence of the cis-motifs of individual members of each family may vary for each gene. Not drawn to scale. (b) H4 H3 H 2A H2B H4 H3 H2B H 2A TATA TATA TATA TATA NEG NEG SPT10 SPT10 ... fixation of adaptive variants by positive selection. Adaptive expression changes of this type may be facilitated by the emergence of intermediate redunda...
Ngày tải lên: 14/08/2014, 17:22
Báo cáo y học: "Long Term Persistence of IgE Anti-Influenza Virus Antibodies in Pediatric and Adult Serum Post Vaccination with Influenza Virus Vaccine"
... were determined by sandwich ELISA (Bio- source, Camarillo, CA) according to the manufactur- er’s protocol. Statistical Analysis Cytokine determinations from vaccinated and non vaccinated subjects ... cold-adapted (CA) live, attenuated vaccines are ad- ministered intranasally in children and provide pro- tective immunity [21]. After CA vaccination, serum antibody responses and/or virus...
Ngày tải lên: 25/10/2012, 11:10
Tài liệu Báo cáo Y học: Exploring the role of a glycine cluster in cold adaptation of an alkaline phosphatase pdf
... 2002 Exploring the role of a glycine cluster in cold adaptation of an alkaline phosphatase Konstantinos Mavromatis 1, *, Iason Tsigos 2, *, Maria Tzanodaskalaki 2 , Michael Kokkinidis 1,3 and Vassilis ... the same as in the case ofthenativeenzyme(Table1). Thermal inactivation of mutant and wild-type enzymes In order to investigate the effects of mutations on the stability of...
Ngày tải lên: 22/02/2014, 04:20
Báo cáo Y học: Isolation and characterization of a thioredoxin-dependent peroxidase from Chlamydomonas reinhardtii doc
... identity with the BAS1 protein of Brassica, spinach, barley and A. thaliana,PR1ofPhaseolus and MHF 9 of A. thaliana. These proteins b elong to the 2Cys-Prx subfamily. A ll plan t 2Cys-Prx proteins, ... with an online phenylthiohydantoin amino-acid analyser. F or MALDI-TOF analysis, 1 lL of tryptic digest or HPLC-puri®ed peptides was mixed with 1 lLof saturated solution of a- cyan...
Ngày tải lên: 08/03/2014, 16:20
Báo cáo Y học: The regulatory subunit of a cGMP-regulated protein kinase A of Trypanosoma brucei docx
... least in some instances, the activity of mammalian PKA appears to be stimulated by cGMP rather than by cAMP [22]. In the unicellular eukaryote Trypanosoma brucei, the causative agent of human ... exhibiting many character- istics of the catalytic subunit of trypanosomal PKA. The coprecipitated kinase is recognized by an antibody against the bovine PKA catalytic subunit, the enzyme...
Ngày tải lên: 31/03/2014, 23:20