Báo cáo khoa học: " Systemic hypothermia increases PAI-1 expression and accelerates microvascular thrombus formation in endotoxemic mice" ppsx

... patients. Conclusion Systemic hypothermia superimposed on endotoxemic chal- lenge further increases microvascular thrombus formation in vivo. This involves an increase in circulating PAI-1 expression rather ... in a marked increase in the expression of P-selectin and PAI-1 within the microvascular endothelium. In endotoxemic animals endothelial PAI-1 e...

Ngày tải lên: 13/08/2014, 03:20

... determined by absorbance at 275 nm using e 275 = 1400 m )1 cm )1 . Assessment of aggregation using thioflavin T binding and electron microscopy The kinetics of fibril formation was determined using ... by silver staining. In the examples shown, 100 ng of each peptide were loaded per lane, and only a single band was detected in the lanes containing Ab(1–40) and Ab(M1–40) (Fig. 5E)....

Ngày tải lên: 18/02/2014, 13:20

... of proteins involved in retinoid-related metabolism (retinoic acid receptors, cellular binding proteins and oxidizing enzymes) have been mapped within the adult dopamine system [35]. In the adult ... implications in the metabolism of ethanol and retinoic acid. In vitro and in vivo data indicate that ADH1, ADH3 and ADH4 can oxidize retinol to retinal, with ADH4 having very hi...

Ngày tải lên: 21/02/2014, 00:20

... galectin 3, an S-type lectin containing a carbohydrate-binding domain, as a physiological ligand of CD98 in vivo [18]. Figure 5A shows the primary intracellular distribu- tion of galectin 3 in the ... and in intestine [26]). Finally, there is evidence for galec- tin 3 as a factor in RNA splicing, based on the local- ization of the protein in the nucleus [27]. In this article, we...

Ngày tải lên: 23/03/2014, 09:20

... accompa- nied by a reduction in the amount of cytosolic Sp1. In order to determine whether glutamine increases the binding of Sp1 to the SREBP-1a promoter region containing the Sp1-binding elements, we performed ... pathway plays a key role in SREBP-1 processing in the presence of glutamine but not in glutamine-stimulated SREBP-2 processing. Recent reports indicate that insulin en...

Ngày tải lên: 28/03/2014, 22:21

... pneu- moniae-induced overexpression of PAI-1 in vascular smooth muscle cells and endothelial cells [39]. Furthermore, NF-jB-like binding site in the PAI-1 promoter is responsible for IL-1-induced PAI-1 expression ... Thymosin beta 4 induces the synthesis of plasminogen activator inhibitor 1 in cul- tured endothelial cells and increases its extracellular expression. Blood...

Ngày tải lên: 30/03/2014, 11:20

... proteins. The selective labeling of proteins by cell-free expression is highly efficient and advantageous compared with the in vivo labeling. No auxotrophic strains and minimal media are needed, and ... were combined, washed in 1% (v/v) NM and in 1% (v/v) DPC and dissolved in 2 mL of 1% (v/v) MHPG in 25 m M sodium phosphate, pH 6.0, with 5 m M dithiothreitol. After remova...

Ngày tải lên: 30/03/2014, 13:20

... listeriosis. The involvement of iNOS in bovine and caprine listeriosis has been reported previously, and the increased expression of iNOS and the resultant NO generation were confirmed in brains with ... role in the pathogenesis of arthritis and spinal cord inflammation. In summary, these results suggest that both nNOS and iNOS are constitutively expressed in brain cell...

Ngày tải lên: 07/08/2014, 14:23

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Ngày tải lên: 07/08/2014, 17:22

... protection standards for PM by adding an indicator for even “fine” particles (PM2.5). In this trend, our government is trying to update on ongoing litigation over PM2.5 standard. However, relative less information ... collected and removed the biological species such as pollen and endotoxin. Collected PM s were weighted and resuspended in PBS solution. Resuspension took place in...

Ngày tải lên: 07/08/2014, 17:22