Báo cáo y học: "Transcriptome analysis of monocyte-HIV interaction" pot

... levels of many of these genes identified in the array analysis. Moreover, Western blot analysis of thymo- cyte lysates also confirmed that IgM increases with age (Figure 3). Flow cytometry analysis ... and array analysis. For each array sample, the RNA was prepared from the purified thymocytes. The thymocytes were processed by using the RNEasy Mini Kit (Qiagen, Valencia, CA)....

Ngày tải lên: 03/11/2012, 11:35

... char- acteristics of types A and B of Emiliania huxleyi (Prymnesio- phyceae) as determined by flow cytometry and chemical analyses. J Phycol 1994, 30:230-241. 22. Wahlund TM, Hadaegh AR, Clark R, Nguyen B, ... synchroneity of late quaternary coccolith datum levels - validation by oxygen isotopes. Geology 1977, 5:400-404. 7. Paasche E: A review of the coccolithophorid Emiliania huxleyi...

Ngày tải lên: 09/08/2014, 20:20

... underlying this detr imental form of immu- nopathology. Two genes in particular may be of func- tional interest: upregulation of CYP2C19, a member of the cytochrome P450 monooxygenase family with ... expressed differentially in monocytes from HIV patients versus healthy controls, as assessed by CodeLink HWG microarray analysis. Samples analysed by CodeLink HWG microarray were grouped...

Ngày tải lên: 13/08/2014, 01:20

... to identify key molecules in the course of hy- poxia-induced apoptotic pathways. Such analysis of trophoblastic cells will, in the near future, yield in- sights into the mechanisms of preeclampsia ... biology and biochemistry have enabled analy- sis of the expression profiles of numerous proteins at once. In this study, we looked at hypoxia-induced apoptosis and, by using the...

Ngày tải lên: 31/10/2012, 15:12

... of hCDC4 can also be observed in Acute Myeloid Leukemia (AML) or high risk Myelodysplastic Syndrome (MDS). Therefore we carried out a mutational analysis of the hCDC4 gene in 35 samples of ... Results Mutational analysis of hCDC4 in AML In this study the common exons 2 to 11 and the three known transcript variants of exon 1 of hCDC4 (Figure 1A) were analyzed by direct...

Ngày tải lên: 31/10/2012, 16:49

... potentiated by enzymatic degradation of phospholipids [7,44]. The capacity of DM64 to inhibit the myotoxicity induced by myotoxins I (Asp49) and II (Lys49) from B. asper venom was analyzed in vivo ... three domains and are present in the inhibitory receptors of Fig. 6. Inhibition of in vitro cytotoxicity of myotoxins I or II by DM64. Cytotoxicity was analyzed in vitro using C2C12 sk...

Ngày tải lên: 21/02/2014, 01:21

... H.S., Han, Y. & Fung, B.K K. (2000) Subunit e xchange of small h eat sho ck proteins. Analysis of olig- omer formation of aA-crystallin and Hsp27 by fluorescence res- onance energy transfer ... chaperoning in vitro [66], and disassembly of active units from an oligomeric (storage) state of a-crystallin was proposed, upon structural analysis of aA-crystallin by site- direct...

Ngày tải lên: 22/02/2014, 04:20

... formation of gamma glutamyl-lysine bonds [4]. Furthermore, tTG plays a role in the transduction of extracellular signals, mediated by its additional GTP- hydrolyzing activity [5], analogous to that of ... member of a family of seven isoforms of enzymes involved in protein cross-linking, including prostatic TGase and factor XIII. tTG is a Ca 2+ -dependent ubiquitous intracellular e...

Ngày tải lên: 08/03/2014, 09:20

... 2-N-(3-O- acyl 4 -acyl 2 )-b-GlcN-(1–6)-2-N-acyl 1 )3-O-acyl 3 -GlcN where acyl 1 ,acyl 2 and acyl 3 are 3-hydroxyhexadecanoic or 3-hy- droxyoctadecanoic acids, acyl 4 is tetradecanoic or (minor) hexadecanoic ... Acyl 1 ,acyl 2 and acyl 3 residues had hydroxy or acyloxy groups at C-3 ( 13 C signals of C-3 at 69.0–72.4 p.p.m.), while acyl 4 had no substituents. The signals of acyl chains c...

Ngày tải lên: 08/03/2014, 09:20

... chemical analysis of 7-O-(2-amino-2-deoxy-a- D -glucopyranosyl- L -glycero- D -manno-heptose as a constituent of the lipopolysaccharides of the UDP-galactose epimerase less mutant J-5 of Escherichia ... described previously [13]. Preparation of deacylated LPS of E. coli F653 LPS (2.11 g) was de-O-acylated by mild hydrazinolysis (yield 1.425 g) and 902.5 mg were further subjected...

Ngày tải lên: 08/03/2014, 09:20