Báo cáo khoa học: " Recruitment maneuvers and positive end-expiratory pressure/tidal ventilation titration in acute lung injury/acute respiratory distress syndrome: translating experimental results to clinical practice" ppsx

... determine the working envelope in the presentbreath and the chances of promoting intratidal recruitment during mechanical ventilation in ARDS patients.Overdistension and the opening and closing ... CSV, Matos GFJ, Pincelli MP, Borges ER, Antunes T,Barros JM, Okamoto V, Borges JB, Amato MBP, Carvalho CRR:Mechanical ventilation in acute respiratory failure: recruitment and high positive end-expiratory ... injury /acute respiratory distress syndrome (ARDS). (a) Computed tomography (CT)scan of pigs after saline lung lavage before and after recruitment maneuvers with 45 cmH2O of pressure, maintaining a positive...

Tài liệu Báo cáo khoa học: Complex transcriptional and translational regulation of iPLA2c resulting in multiple gene products containing dual competing sites for mitochondrial or peroxisomal localization docx

... 2004rate-determining factor in uncoupling protein (UCP) activ-ity [16]. Thus, t he role of mitochondrial i PLA2activities in regulating mitochondrial function is just now beginning to be understood. ... heat-ing a 4-lMmixture of primers to 95 °C for 3 min followedby cooling to 22 °C prior to cloning into the Xho1/Sal1sites of vector pEGFP-N3. Integrity and orientation of theN-terminal fusion ... to allow i PLA2c to participate in regulating lipid metabolism in multiplecompartments thereby contributing to orchestrating energystorage, u tilization and signal transduction pathways in multiple...

Báo cáo khoa học: Proteolytic activation and function of the cytokine Spatzle in the innate immune response of a lepidopteran insect, Manduca sexta ppt

... Immunity-relatedgenes and gene families in Anopheles gambiae. Science298, 159–165.20 Shin SW, Bian G & Raikhel AS (2006) A toll receptor and a cytokine, Toll5A and Spz1C, are involved in tollantifungal ... Spe I and XhoI, and then inserted into the same restriction sites in the vectorpFastBac1 (Invitrogen). The resulting plasmid, after sequenceveriﬁcation, was used to generate a recombinant baculovirusaccording ... patterning in earlyembryonic development and in the antimicrobial immune response in larvae and adults. We have investigated the function of Spa¨tzle in a lepidopteraninsect, Manduca sexta, in...

Báo cáo khoa học: Puriﬁcation and characterization of the cysteine proteinases in the latex of Vasconcellea spp. ppt

... manufac-turer’s instructions. Insert sequencing was carried out in both directions using T7 and SP6 primers. Inserts were se-quenced using the ABI prism BigDyeTMTerminator v1.1Cycle Sequencing Kit ... conservedP2,Q19 and the11GAVTP15-motif located at the N-ter-minus of papain-like cysteine proteinases, leaving nodoubt that these proteins belong to the papain super-family. Sequencing of VXH-I and ... response to wounding of the plant [26].Similar to other known cysteine proteinases from Car-icaceae, the deduced protein sequences of VXH-A, -B,-C and -D, and VS-A and -B, were predicted to con-tain...

Báo cáo khoa học: Puroindoline-a and a1-purothionin form ion channels in giant liposomes but exert different toxic actions on murine cells pptx

... action, together with membrane blebbing and disorganization and disarray of cytoskeletal actin, mayrepresent apoptotic events involved in a1-PTH cyto-toxicity. Experimental proceduresExtraction and ... involved in the toxicity of PTHs and PINs to mammalian cells remain poorly understood. There-fore, as a ﬁrst step toward understanding these mecha-nisms, we characterized (a) the pore-forming ... as a single peak justafter the PINs. Separate pools of the PTH-containing and PIN-containing crude fractions were dialyzed against de-ionized water and freeze-dried, and a1-PTH, a2-PTH and b-PTH...

Báo cáo khoa học: "Combining Statistical and Knowledge-based Spoken Language Understanding in Conditional Models" pptx

... optimization, Springer-Verlag. Povey, D. and P. C. Woodland. 2002. Minimum phone error and I-smoothing for improved discriminative training. IEEE International Conference on Acoustics, Speech, and Signal ... directly porting the model to HCRFs, and finally introduce the CRFs and 882Figure 3 is deterministic, as shown in Figure 4. However, the words are not aligned to the states in the shaded ... joint expertise in natural language processing and speech recognition, and best practices in language engineering for every new domain. On the other hand, a statistical learning approach needs...

Báo cáo khoa học: "Combining Stochastic and Rule-Based Methods for Disambiguation in Agglutinative Languages" pptx

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Báo cáo khoa học: Antioxidant defences and homeostasis of reactive oxygen species in different human mitochondrial DNA-depleted cell lines pot

... (Fe–S)-containing hydratase, present in various subcellularcompartments (i.e. mitochondria and cytosol) which isinactivated by OÆ À2[41]. In the cytosol, loss of aconitaseactivity results in the ... o f MnSOD, CuZ nSOD and actin, the last acting as an internal standard, and incubated with thecorresponding antibody. Forty micrograms of cell protein extrac t wasloaded in ea ch lane. The blots ... during electrontransport is reduced not to water by cytochrome c oxidasebut rather to superoxide by complexes I, and III of the respiratory chain [1,7,8]. ROS production increases w henrespiratory...

Báo cáo khoa học: Identiﬁcation and characterization of novel PKA holoenzymes in human T lymphocytes pdf

... kDa band in T cells. ACb-speciﬁc antibody anti-Cb(SNO157) (vi) recognized the 47 kDa band in T-cells, and the 40- and 47 kDa protein bands in Jurkat cells. (C) Lin-ear depiction of Ca1, Cb1 and ... analoguewas included to avoid potential binding of non-Cb2C-subuints to the R-subunits. The resulting precipitateswere analysed by SDS ⁄ PAGE and immunoblottingusing anti-Cmono (Fig. 2B) and a PKA-speciﬁc ... thatcould be inhibited by protein kinase inhibitor (PKI). In the presence of cAMP approximately one-third of thePKI-inhibited activity was released into the extract, indi-cating that the precipitated...

Báo cáo khoa học: "THE SYNTAX AND SEMANTICS OF USER-DEFINED MODIFIERS IN A TRANSPORTABLE NATURAL LANGUAGE PROCESSOR" pot

... most meaningful interpretations of phrases containing "non" or "not" can be obtained by inserting the retrieval r2.odule's Not command at an appropriate point in the ... that can occur in the "layered" domains of interest [1]. Finally, the macro file contains the meanings of modifiers, roughly in the form in which they were acquired using the specification ... shown in Figure i, the English-language processor of LDC achieves domain independence by restricting itself to (a) a domain-independent. linguistically-motivated phrase-structure grammar [6] and...

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