... times of adenosine and adenine, inosine and hyp-oxantine, guanosine and guanine, uridine and uracil, cyti-dine and cytosine were 12.2 and 6.2 min, 10.5 and 4.7 min,15.2 and 6.1 min, 6.8 and ... and 4.2 min and 6.6 and 3.9 minrespectively. The amount of purine or pyrimidine baseformed is determined by integrating the peak of producednucleobase and converting this to the amount of nucleobaseby ... Thermolysin was obtained fromBoehringer (Mannheim, Germany). Trypsin, proteinase K,subtilisin, nucleosides, purine and pyrimidine bases,O-bromoacetyl-N-hydroxysuccinimide and standard pro-teins...