Báo cáo khoa học: " In vitro analysis of expression vectors for DNA vaccination of horses: the effect of a Kozak sequence" docx

... AnI19R 5¢-AATTCATGAGGAGGTTTCTCTGTAACA-3¢;AnI19H 5¢-AGCTTGTTACAG-AGAAACCTCCTCATG-3¢; AnI17R 5¢-AATTCACGAGGAGGTTTCTCTGTACTA-3¢; AnI17H 5¢-AGCTTAGTACAGAGAAACCTCCTCG TG-3¢;AnI15R5¢-AATTCACAGGAGGTTTCTCT-GTC TA-3¢;AnI15H5¢-AGCTTAGACAGAGAAACCTCC TGTG-3¢. Each annealed ... to I-AniI before addition of the DNA substrate. To determine whether the RNA substrate can inhibit the cleavage of pr...

Ngày tải lên: 17/03/2014, 10:20

... tissues, and thus there are special demands for the sealing agent s [31]. The major task in reducing the bacterial load, concentrated in the apical region of the root canal, is achieved by the mechanical ... sea- lers have already been assessed for a possible antibacter- ial effect, most often measured against strains of E. faecalis [21-28]. Baer and Maki [29] demon...

Ngày tải lên: 11/08/2014, 20:21

... not for citation purposes) Acta Veterinaria Scandinavica Open Access Research In vitro analysis of expression vectors for DNA vaccination of horses: the effect of a Kozak sequence Guðbjörg Ólafsdóttir 1 , ... 5'-GTATGCGGCCGCCACCATGAAGT- GGGTAACCTTTAT-3' with Kozak sequence and 5'- CGCTAGGATCCAATCAATGGTGATGGTGATGATG-3'. Taq DNA Polymera...

Ngày tải lên: 12/08/2014, 18:22

... DNA drug complex [33]. The significant change in the band at 250 and 280 nm implied an alteration in the DNA conformation because of an overall bending of the DNA backbone [33,35]. Both DDI- 1A and ... not affect DNA slippage (data not shown). Interestingly, there was a similar hierarchy of intensities for the three bands in the (AAT) 3 ⁄ (ATT) 5 and (ATT) 3...

Ngày tải lên: 07/03/2014, 06:20

... Yasuda J, Mashiyama S, Makino R, Ohyama S, Sekiya T & Hayashi K (1995) Cloning and characterization of rat cellular nucleic acid binding protein (CNBP) cDNA. DNA Res 2, 45–49. 13 Tomonaga T, Michelotti ... open La autoantigen (La)-bound form would, instead, allow translation. Cell signals might in uence the affinities of La or CNBP for the 5¢ UTR, and the alternative binding...

Ngày tải lên: 16/03/2014, 12:20

... mass of the CTF migrating faster than C101-3FLAG as an a- CTF within 27 Da of the calculated molecular mass. Finally the same line was used to predict the molecular mass of a third FLAG-reactive ... c-3FLAG standard. Finally, the pri- mer pairs forward, 5¢-GGGGGGCCATGGTGATGCTGA AGAAGAACAG-3¢ and reverse 3FLAG HindIII were used to generate the plasmid e-3FLAG standard. Pre...

Ngày tải lên: 16/03/2014, 23:20

... cerevisiae, that the l -a- aminoadipate substrate could be adenyl- ated at the d-carboxylate rather than the a- carboxy- late and that the a- amino and a- carboxyl groups of the substrate bind at the bottom of the ... the A and T domains from NRPS systems and the C-terminal part contains a redox cofactor binding site for either pyrroloquinoline quinone (PQQ) or...

Ngày tải lên: 23/03/2014, 13:20

... that inactivates the protein. The self-cleavage of LexA protein is important in the SOS response in bacteria [21–23]. In vitro self-cleavage was also observed in all investigated bacterial SPase ... its substrates. Therefore, the self-cleavage of SPase I is believed to inactivate the activity of the enzyme. In vivo self-cleavage of S. pneumoniae SPase I As the...

Ngày tải lên: 23/03/2014, 21:21

... selection methods remains largely untested. We have investigated plasmid display as a suitable in vitro format by engineering a variant of phosphoribosylanth- ranilate isomerase (PRAI) that carried the FLAG epitope ... comparing the far-UV and near-UV CD spectra of PRAI and FLAG-PRAI in a buffer in which PRAI retains catalytic activity (Fig. 3). The spectra are effecti...

Ngày tải lên: 30/03/2014, 20:20

... washing away the chelator by Table 1. Effect of citrate, malate and NaCl on PTOX activity in vitro. Assays were performed in the presence of NADH, decyl-plastoqui- none and KCN as described in the ... concentration of NaCl was also increased in a separate assay. PTOX activity is given as percentage of starting O 2 consumption (before addition of these compounds). T...

Ngày tải lên: 31/03/2014, 07:20