Báo cáo khoa học: "Isolation and characterization of Treponema phagedenis-like spirochetes from digital dermatitis lesions in Swedish dairy cattle" pps

... ScandinavicaOpen AccessResearchIsolation and characterization of Treponema phagedenis-like spirochetes from digital dermatitis lesions in Swedish dairy cattleMärit Pringle*1, Christer Bergsten2, ... coronet. Spirochetes of the genus Treponema are found in high numbers in the lesions and are likely to beinvolved in the pathogenesis. The aim of this study was to obtain pure cultures of spirochetes ... an MIC of 4–8 μg/ml for the remaining isolates.Tylosin (a macrolide) and lincomycin (a lincosamide)have overlapping binding sites on the ribosome and theMICs often follow each other. Compared...

Tài liệu Báo cáo khoa học: Isolation and characterization of four type 2 ribosome inactivating pulchellin isoforms from Abrus pulchellus seeds docx

... relationto carbohydrate binding. From the putative C-terminalsugar binding site (identical in P I and P II but differ-ing by a single residue (Trp instead of Tyr) in bothP III and P IV), the same ... chains of ricin, a toxicprotein inhibiting protein synthesis. Biochemistry 12,3121–3126.21 Olsnes S & Pihl A (1973) Isolation and properties of abrin: a toxic protein inhibiting protein ... suboptimal binding of GalNAc in one or bothbinding pockets of P I. However, in striking contrastwith the rescue of protein synthesis observed for P I and P II, protection against P III and P IV...

Báo cáo khoa học: Isolation and characterization of a D-cysteine desulfhydrase protein from Arabidopsis thaliana pptx

... sitebetween amino acids 19 and 20 counting from the startmethionine was predicted, indicating a presequence of 19 amino acids. The mature protein would have amolecular mass of 41.7 kDa and a pI of 6.34.The ... highprobability of occurrence.Enzyme activity of the recombinant proteinThe recombinant Arabidopsis d-CDes proteins inclu-ding and excluding the targeting peptide were expressed in E. coli and already ... b-chloro-d-alanine and b-chloro-l-alanine were used in the O-acetyl-l-serine(thiol)lyase (OAS-TL) assay instead of O-acetyl-l ⁄ d-serine and the formation of cysteine wasdetermined; the d-CDes protein...

Báo cáo khoa học: Isolation and characterization of an IgNAR variable domain speciﬁc for the human mitochondrial translocase receptor Tom70 potx

... whetherbinding afﬁnity was retained in the absence of the conservedimmunoglobulin intradomain disulphide bond. In an initialseries of experiments, recombinant 12F-11 protein wasdenatured and reduced ... allowingformation of a large number of conformational antigen-binding surfaces including planar and ridged topologies [2].The orientation of the loops is maintained by a combination of their internal ... As an initial step in producing ÔintrabodyÕ variants of 12F-11, the impact of modifying or removing the conserved immunoglobulinintradomain disulphide bond was assessed. High aﬃnitybinding was...

Báo cáo khóa học: Isolation and characterization of the Xenopus HIVEP gene family ppt

... bythe generation of deletion mutants containing discretefunctional domains of XHIVEP1. Thus, the cloning and characterization of the XHIVEP interacting factors wouldbe of interest and should also ... BMP signaling (data not shown). While themutations were sufﬁcient to disrupt binding of the zincﬁnger pair, they may not be capable of inhibiting binding of the full length protein. Additionally, ... organization of the XHIVEP proteins. Black barsindicate the position of the ZAS C2H2zinc ﬁngers (ZAS-N and ZAS-C) and the isolated C2HC zinc ﬁnger (I-Znf). The ZAS zinc ﬁngerDNA binding domains...

Báo cáo khoa học: " Isolation and characterization of Streptococcus sp.from diseased flounder (Paralichthys olivaceus )in Jeju Island" ppt

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Báo cáo khoa học: " Isolation and characterization of canine umbilical cord blood-derived mesenchymal stem cells" pps

... cardiomyocytes and osteoblasts in vitro and in vivo under controlled conditions [15,24,27]. These cells can be isolated from many kinds of tissues, including fat, skin, and even the brain [2,13,18,22]. ... shown in pellet formation and toluidine blue staining. In this study, we used cUCB-MSCs at 3∼5 passage. Generally, increasing the passage number of adult stem cells often leads to a decline in ... the bottom of the tube (Fig. 3D), and positive to toluidine blue staining (Figs. 4E and F).DiscussionIsolation and characterization of stem cells derived from various tissues and sources...

Báo cáo khoa học: "Isolation and characterization of a genotype 4 Hepatitis E virus strain from an infant in China" ppt

... far from the genotype 4 HEV strain that was commonly prev-alent in Shanghai swine group, suggesting that this strainmay not come from swine group and not involved in zoonotic transmission in ... sequence of the isolate in this study and other 10 genotype 4 HEV isolates in China, using the neighbor-joining method and evaluated using the interior branch test method with Mega 4 softwareFigure ... sequence of the isolate in this study and other 10 genotype 4 HEV isolates in China, using the neighbor-joining method and evaluated using the interior branch test method with Mega 4 software....

Báo cáo khoa học: Expression and characterization of the protein Rv1399c from Mycobacterium tuberculosis A novel carboxyl esterase structurally related to the HSL family docx

... puriﬁedenzyme was incubated with different ratio of E600. In eachcase, the kinetics of inactivation of Rv1399c have beenmonitored b y measuring the remaining activity, a s afunction of time, using tripropionin ... LBmedium after 4 h of IPTG induction (Fig. 1).Puriﬁcation and refolding of Rv1399cGiven the large quantity of insoluble material obtained [upto 160 mg of protein from 6 L of cell culture (Table ... vectorfollowing the manufacturer’s instruction (Gateway, Invi-trogen). The correct insertion of the Rv1399c ORF wasconﬁrmed by DNA sequencing. In our hands, protein expression using the commerciallyavailable...

Báo cáo khoa học: "Purification and characterization of two larval glycoproteins from the cattle tick, Boophilus annulatus" doc

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