Báo cáo khoa học: "Isolation and characterization of Treponema phagedenis-like spirochetes from digital dermatitis lesions in Swedish dairy cattle" pps

... Scandinavica Open Access Research Isolation and characterization of Treponema phagedenis-like spirochetes from digital dermatitis lesions in Swedish dairy cattle Märit Pringle* 1 , Christer Bergsten 2 , ... coronet. Spirochetes of the genus Treponema are found in high numbers in the lesions and are likely to be involved in the pathogenesis. The aim o...

Ngày tải lên: 12/08/2014, 18:22

... relation to carbohydrate binding. From the putative C-terminal sugar binding site (identical in P I and P II but differ- ing by a single residue (Trp instead of Tyr) in both P III and P IV), the same ... chains of ricin, a toxic protein inhibiting protein synthesis. Biochemistry 12, 3121–3126. 21 Olsnes S & Pihl A (1973) Isolation and properties of abrin: a toxic protein...

Ngày tải lên: 18/02/2014, 16:20

... site between amino acids 19 and 20 counting from the start methionine was predicted, indicating a presequence of 19 amino acids. The mature protein would have a molecular mass of 41.7 kDa and a pI of 6.34. The ... high probability of occurrence. Enzyme activity of the recombinant protein The recombinant Arabidopsis d-CDes proteins inclu- ding and excluding the targeting pept...

Ngày tải lên: 16/03/2014, 18:20

... whether binding affinity was retained in the absence of the conserved immunoglobulin intradomain disulphide bond. In an initial series of experiments, recombinant 12F-11 protein was denatured and reduced ... allowing formation of a large number of conformational antigen- binding surfaces including planar and ridged topologies [2]. The orientation of the loops is maintained by...

Ngày tải lên: 17/03/2014, 10:20

... by the generation of deletion mutants containing discrete functional domains of XHIVEP1. Thus, the cloning and characterization of the XHIVEP interacting factors would be of interest and should also ... BMP signaling (data not shown). While the mutations were sufficient to disrupt binding of the zinc finger pair, they may not be capable of inhibiting binding of the full leng...

Ngày tải lên: 30/03/2014, 13:20

Ngày tải lên: 07/08/2014, 18:21

... cardiomyocytes and osteoblasts in vitro and in vivo under controlled conditions [15,24,27]. These cells can be isolated from many kinds of tissues, including fat, skin, and even the brain [2,13,18,22]. ... shown in pellet formation and toluidine blue staining. In this study, we used cUCB-MSCs at 3∼5 passage. Generally, increasing the passage number of adult stem ce...

Ngày tải lên: 07/08/2014, 23:22

... far from the genotype 4 HEV strain that was commonly prev- alent in Shanghai swine group, suggesting that this strain may not come from swine group and not involved in zoonotic transmission in ... sequence of the isolate in this study and other 10 genotype 4 HEV isolates in China, using the neighbor-joining method and evaluated using the interior branch test method with...

Ngày tải lên: 12/08/2014, 04:21

... purified enzyme was incubated with different ratio of E600. In each case, the kinetics of inactivation of Rv1399c have been monitored b y measuring the remaining activity, a s a function of time, using tripropionin ... LB medium after 4 h of IPTG induction (Fig. 1). Purification and refolding of Rv1399c Given the large quantity of insoluble material obtained [up to 160 mg of...

Ngày tải lên: 07/03/2014, 16:20

Ngày tải lên: 07/08/2014, 20:23