Báo cáo y học: "Binding of protegrin-1 to Pseudomonas aeruginosa and Burkholderia cepacia" potx

... TSB, and 1% BSA. Samples were layered over 0.3 ml of a cush- ion composed of 5 parts of dibutyl phthalate and 3 parts of di-isodecyl phthalate (density 20 = 1.01) and centrifuged at approximately ... Antimicrobial and cytotoxic peptides of mammalian cells. Annu Rev Immunol 1993, 11:105-128 15. Wang J, Lory S, Ramphal R, Jin S: Isolation and characterization of Pseudomo...

Ngày tải lên: 12/08/2014, 18:20

... with Band Pass (BP) filter and Long Pass (LP) 590 nm filter. RESULTS Binding of Yb 3+ to apo-hTf The thermodynamics of binding of Yb 3+ to apoTf. Binding of Yb 3+ to apo-Tf was investigated by addition of ... l M and K d2 ¼ 18.5 ± 7.0 l M . ICP-AES was also used to study the binding ratio of Yb 3+ to apo-Tf. After addition of 2.0 and 2.5 mol equiv of Yb 3+ to a...

Ngày tải lên: 08/03/2014, 09:20

... ability of hemolin to bind to hemocytes may promote phagocytosis and the formation of hemo- cyte nodules to clear micro-organisms from the insect hemolymph. Recognition of micro-organisms by pattern-recognition receptors ... of bacteria and yeast by hemolin may be due to binding and crossing-linking of these micro-organisms by hemolin. Binding of hemolin to bacteri...

Ngày tải lên: 31/03/2014, 09:20

... studies of the interaction of the tropomyosin-troponin complex with actin and the proteolytic fragments of myosin. J. Biol. Chem. 246, 4866–4871. 19. Roustan, C., Benyamin, Y. , Boyer, M., Bertrand, ... proteolysis of the complex by V8 protease or on cross-linking of the 159–193 peptide to actin after digestion by thrombin. Digestion of actin by V8 protease gives two major fr...

Ngày tải lên: 22/02/2014, 07:20

... lack of steric h indrance created usually by the hydroxyl g roup of Ser146 in the w ild-type enzyme, results in a larger binding cavity, capable of accommodation of lipid analogues normally not ... lack of a hydroxyl group of Ser146 diminishes the role of this residue in the stabilization of the substrate– ligand molecule, leading to the ÔconfusionÕ of the enzyme in the pr...

Ngày tải lên: 08/03/2014, 23:20

... & Yamazaki RK (2003) A critical role for ATP in the stimulation of retinal guanylyl cyclase by guanylyl cyclase-activating proteins. J Biol Chem 278, 33150–33160. 36 Yamazaki A, Yamazaki M, Yamazaki ... Kachi S, Maeda T, Yamamoto M, Fujii Y, Liu H, Yamazaki M, Usukura J & Yama- zaki A (2000) Phosphorylation by cyclin-dependent pro- tein kinase 5 of the regulatory subunit of ret...

Ngày tải lên: 06/03/2014, 00:21

... center of the G-tetrad in the region of high negative charge density generated by the carbonyl groups. Extension of this study to other known and well-established ligands, such as porphyrin and ... DNAs. Biochemistry 45, 6681– 6691. 16 Mita H, Ohyama T, Tanaka Y & Yamamoto Y (2006) Formation of a complex of 5,10,15,20-tetrakis (N-methyl- pyridinium-4-yl)-21H, 23H-porph...

Ngày tải lên: 16/03/2014, 06:20

... AcnB and was hydrated to (2R,3S)-2-methylisocitrate. This product was cleaved by PrpB into succinate and pyruvate (Fig. 1). To monitor the reaction and to pull the equilibrium to the side of pyruvate formation, ... would be of interest to identify the product of the syn hydration of cis-aconitate, perhaps one enantiomer of erythro-isocitrate. No significant activity w...

Ngày tải lên: 17/03/2014, 10:20

... due to the cofactor was significantly lower than that of the wild-type enzyme and, correspond- ingly, the amount of PLP released by NaOH treatment was only 10% of that expected for a fully saturated ... hydrated form of PLP in the mutant enzymes. Conversely, the less hydrated environ- ment of the high-pH conformation favors the 340 nm- absorbing enolimine tautomer of the wild-ty...

Ngày tải lên: 31/03/2014, 08:20

... actions (especially at higher doses) and the genomic actions transrepression and transactivation are not fully synonymous with inflammatory and immunosuppressive activity and with adverse effects, respectively. ... of gluco- corticoids to inflammatory sites using polyethylene glycol liposomes as a vector, increasing local levels and reducing systemic concentrations of glucocor...

Ngày tải lên: 09/08/2014, 01:22