Báo cáo y học: "Binding of protegrin-1 to Pseudomonas aeruginosa and Burkholderia cepacia" potx
Báo cáo y học: "Binding of protegrin-1 to Pseudomonas aeruginosa and Burkholderia cepacia" potx
... TSB, and 1% BSA. Samples were layered over 0.3 ml of a cush-ion composed of 5 parts of dibutyl phthalate and 3 parts of di-isodecyl phthalate (density20 = 1.01) and centrifuged atapproximately ... Antimicrobial and cytotoxic peptides of mammalian cells. Annu Rev Immunol1993, 11:105-12815. Wang J, Lory S, Ramphal R, Jin S: Isolation and characterization of Pseudomonas aeruginosa genes inducible by ... patient to another and its propensity to give rise to the B. cepacia syndrome,which results in a rapid decline in pulmonary function[2,18]. The ability of B. cepacia as well as P. aeruginosa to cause...
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Báo cáo Y học: Complexation of ytterbium to human transferrin and its uptake by K562 cells pot
... with Band Pass (BP) filter and LongPass (LP) 590 nm filter.RESULTSBinding of Yb3+ to apo-hTfThe thermodynamics of binding of Yb3+ to apoTf.Binding of Yb3+ to apo-Tf was investigated by addition of ... lM and Kd2¼18.5 ± 7.0 lM.ICP-AES was also used to study the binding ratio of Yb3+ to apo-Tf. After addition of 2.0 and 2.5 mol equiv of Yb3+ to apo-Tf, the final ratios of Yb3+ to Tf, ... dependence of e (top) and k (bottom) on the molar ratio of Yb3+ to apo-Tf. In the lower panel the data of k1, k2 and k over therange of the ratio [Yb3+]/protein between 2 and 6 are linearly fitted:k1¼...
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Báo cáo khoa học: Binding of hemolin to bacterial lipopolysaccharide and lipoteichoic acid An immunoglobulin superfamily member from insects as a pattern-recognition receptor doc
... ability of hemolin to bind to hemocytesmay promote phagocytosis and the formation of hemo-cyte nodules to clear micro-organisms from the insecthemolymph.Recognition of micro-organisms by pattern-recognitionreceptors ... of bacteria and yeast by hemolin may be due to binding and crossing-linking of these micro-organisms by hemolin.Binding of hemolin to bacteria and yeast cellsM. sexta hemolin was reported to ... display direct antibacterial activity.Instead, its role may be related to its ability t o bind to thesurface of hemocytes [5,13–15] a nd to bacteria. Hemolin hasbeenshowntobindtoE. coli [4,23] and...
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Tài liệu Báo cáo Y học: Binding of gelsolin domain 2 to actin An actin interface distinct from that of gelsolin domain 1 and from ADF/cofilin pptx
... studies of the interaction of thetropomyosin-troponin complex with actin and the proteolyticfragments of myosin. J. Biol. Chem. 246, 4866–4871.19. Roustan, C., Benyamin, Y. , Boyer, M., Bertrand, ... proteolysis of the complex by V8 proteaseor on cross-linking of the 159–193 peptide to actin afterdigestion by thrombin. Digestion of actin by V8 proteasegives two major fragments [19] of 31 and ... inhibitingpolymerization.S2, in contrast, preferably binds to the side of the actinfilament. Severing activity seems to require the binding of S2 to the filament, followed by interaction of S1 betweentwo...
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Báo cáo Y học: Binding of Thermomyces (Humicola) lanuginosa lipase to the mixed micelles of cis-parinaric acid/NaTDC Fluorescence resonance energy transfer and crystallographic study ppt
... lack of steric h indrance created usually bythe hydroxyl g roup of Ser146 in the w ild-type enzyme,results in a larger binding cavity, capable of accommodation of lipid analogues normally not ... lack of a hydroxyl group of Ser146 diminishes therole of this residue in the stabilization of the substrate–ligand molecule, leading to the ÔconfusionÕ of the enzyme inthe process of the ligand ... quenching of the tryptophyl fluorescence of model compounds and of lysozyme by iodide ion. Biochemistry 10, 3254–3263.26. Otwinowski, Z. & Minor, W. (1997) Processing of X-ray diffrac-tion data...
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Báo cáo khoa học: Binding of cGMP to the transducin-activated cGMP phosphodiesterase, PDE6, initiates a large conformational change involved in its deactivation ppt
... & Yamazaki RK (2003) A critical rolefor ATP in the stimulation of retinal guanylyl cyclaseby guanylyl cyclase-activating proteins. J Biol Chem278, 33150–33160.36 Yamazaki A, Yamazaki M, Yamazaki ... Kachi S, Maeda T, YamamotoM, Fujii Y, Liu H, Yamazaki M, Usukura J & Yama-zaki A (2000) Phosphorylation by cyclin-dependent pro-tein kinase 5 of the regulatory subunit of retinal cGMPphosphodiesterase. ... inactive form of rod PDE is composed of a cat-alytic subunit complex, Pab, and two inhibitory subun-its, Pcs, i.e. Pabcc [4–10]. A study using electronmicroscopy and image analysis of single particles...
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Báo cáo khoa học: Binding of berberine to human telomeric quadruplex – spectroscopic, calorimetric and molecular modeling studies pot
... center of the G-tetrad in theregion of high negative charge density generated bythe carbonyl groups. Extension of this study to otherknown and well-established ligands, such as porphyrin and ... DNAs. Biochemistry 45, 6681–6691.16 Mita H, Ohyama T, Tanaka Y & Yamamoto Y (2006)Formation of a complex of 5,10,15,20-tetrakis (N-methyl-pyridinium-4-yl)-21H, 23H-porphyrin with G-quadru-plex ... of equilibrium and kinetic measurements to determinethermodynamic origins of stability and specificity and mechanism of formation of site-specific complexesbetween proteins and helical DNA. Methods Enzymol208,...
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Báo cáo Y học: Oxidation of propionate to pyruvate in Escherichia coli Involvement of methylcitrate dehydratase and aconitase pot
... AcnB and was hydrated to (2R,3S)-2-methylisocitrate. This product was cleaved byPrpB into succinate and pyruvate (Fig. 1). To monitor thereaction and to pull the equilibrium to the side of pyruvateformation, ... would be of interest to identify the product of thesyn hydration of cis-aconitate, perhaps one enantiomer of erythro-isocitrate. No significant activity was detected withmany other hydroxy or unsaturated ... aconitase enzymes cata-lyze the conversion of 2-methylcitrate to 2-methylisocitrate.Biochemistry 40, 4703–4713.12. Creighton, D.J. & Murthy, N.R.S.K. (1990) Stereochemistry of enzyme-catalysed...
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Báo cáo Y học: Contribution of Lys276 to the conformational flexibility of the active site of glutamate decarboxylase from Escherichia coli pptx
... due to the cofactor was significantlylower than that of the wild-type enzyme and, correspond-ingly, the amount of PLP released by NaOH treatment wasonly 10% of that expected for a fully saturated ... hydrated form of PLP in themutant enzymes. Conversely, the less hydrated environ-ment of the high-pH conformation favors the 340 nm-absorbing enolimine tautomer of the wild-type cofactor and ... of the holoenzyme by 11 °C, makes it resistant to tryptichydrolysis and prevents the precipitation observed with thewild-type apoenzyme demonstrates that the absence of thislysine residue,...
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Báo cáo y học: "Innovative combination strategy to enhance effect and diminish adverse effects of glucocorticoids: another promise" pot
... actions(especially at higher doses) and the genomic actionstransrepression and transactivation are not fully synonymouswith inflammatory and immunosuppressive activity and withadverse effects, respectively. ... of gluco-corticoids to inflammatory sites using polyethylene glycolliposomes as a vector, increasing local levels and reducingsystemic concentrations of glucocorticoids and thus limitingthe ... instance, hypopituitary-pituitary-adrenal axis suppression and increased risk of infection are linked to transrepression, not to transactivation.Another promising development is the targeting of gluco-corticoids...
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