Báo cáo sinh học: "A new, fast algorithm for detecting protein coevolution using maximum compatible cliques" pptx

... al. Algorithms for Molecular Biology 2011, 6:17 http://www.almob.org/content/6/1/17 Page 2 of 9 RESEARC H Open Access A new, fast algorithm for detecting protein coevolution using maximum compatible ... Press; 1972:85-103. doi:10.1186/1748-7188-6-17 Cite this article as: Rodionov et al.: A new, fast algorithm for detecting protein coevolution using maxim...

Ngày tải lên: 12/08/2014, 17:20

... is an explicit form of the multiple integral, but the explicit form involves inverse of the variance-covariance matrix. Instead of using the explicit form of the integral, ... simulation. With this algorithm, the standard deviation, rather than the variance, of the random effects is estimated using an iterative algorithm similar to the EM a...

Ngày tải lên: 09/08/2014, 18:22

... post-translational modified antigens and therefore to the presentation of conformational-restricted epitopes to the immune system [17]. We previously developed a strategy using recombinant invasive lactococci ... per milliliter): for E. coli, erythromy- cin (100) and chloramphenicol (10); for L. lactis, erythromycin (5) and chloramphenicol (10). DNA manipulations DNA manipulations were...

Ngày tải lên: 14/08/2014, 19:22

... VFP with EGFP and DsRed was performed using clustalw 2 (EMBL-EBI). Homology modeling was carried out using swiss-model [38]. Recombinant protein expression The proteins were expressed in E. coli ... the red chromophore formation in fluorescent proteins and chromoproteins. Chem Biol 11, 845–854. 33 Ward WW (2006) Biochemical and physical properties of green fluorescent protein. In Green F...

Ngày tải lên: 06/03/2014, 11:20

... of 7 (page number not for citation purposes) Acknowledgements The authors wish to thank Dr. Shmuel Pietrokovski for his precious com- ments, Dr. Keizo Nagasaki for the information about their ... Corresponding author Abstract Background: Inteins are " ;protein introns" that remove themselves from their host proteins through an autocatalytic protein- splicing. After their dis...

Ngày tải lên: 18/06/2014, 22:20

... was extracted using the Genomic Mini kit (A&A Biotechnology) according to the manufacturer’s guidelines. The mtDNA D-loop hypervariable region was amplified using the following: forward primer ... direc- tions, using the same primers then for amplification. To check the quality of sequencing, BLAST [1] searches were performed. The sequences were aligned using BioEdit [8] and the G...

Ngày tải lên: 14/08/2014, 13:22

... cm 2) containing a medium similar to that previously described for lymphocyte cultures. G-banding was achieved using a modification of the technique of Seabright (1971). The ... of the First International Conference for the Standardization of Banded Karyotypes of Domestic Animals, Reading, August 2-6, 1976 (Ford CE, Pollock DL, Gustavsson I, eds...

Ngày tải lên: 14/08/2014, 19:22

... chromosome has a double heterochromatic block. Synaptonemal complex analysis was performed using electron microscopy. cattle / chromosome / Robertsonian translocation Résumé - ... associations. In all known cases of centric fusions in cattle, except for the 1/29 translocation, the newly formed biarmed chromosomes have 2 heterochromatic blocks. The 1/...

Ngày tải lên: 14/08/2014, 19:22

... inbreeding and, therefore, a better performance of the candidates for selection, can be obtained maintaining at the same time an important selection response for the commercial ... mated following a criterion of maximum coancestry in order to obtain the 2M candidates for selection in the next generation. ? The values for Nl, n and N were...

Ngày tải lên: 14/08/2014, 20:20

... Therefore, more BLE protein may be produced in C. fusiformis transformants by using a Pfcp-based vector compared with the previ- ously used Pd-containing vectors, which allows more transformants ... in C. fusiformis (N. Kro ¨ ger, unpub- lished observation). Therefore, the promoter of a C. fusiformis fcp gene has been isolated and tested for its applicability in C. fusiformis transforma...

Ngày tải lên: 07/03/2014, 21:20