Báo cáo sinh học: "PhyloScan: identification of transcription factor binding sites using cross-species evidence" ppsx

... the binding of transcription factors (proteins) to cognate sites on a chromosome (tran- scription factor binding sites) . For a given transcription factor and an experimentally identified set of ... for transcription factor binding sites is reduced. A search of such a reduced database in and of itself will allow the detection of more statistically significant...

Ngày tải lên: 12/08/2014, 17:20

... sitesFigure 2 'Fuzziness' of Reb1 binding sites. Average fit of Reb1 binding sites to the consensus matrix, as a function of the number of binding sites within the promoter they appear ... number of binding sites. 0 5 10 1 5 0.35 0.4 0.45 0.5 0.55 0.6 0.65 0.7 Number of binding sites Fraction of 'divergent' promoters Table 2...

Ngày tải lên: 14/08/2014, 15:20

... bona fide targets of the transcription factor [9]. A major challenge in understanding the regulation of transcription is to be able to distinguish real transcription factor binding sites (TFBSs) ... the binding sites of each transcription factor. The evolutionary model we use for TFBSs [25] assumes that sites were under selection to remain binding sites throug...

Ngày tải lên: 14/08/2014, 14:21

... D, Whiteside TL, Kasid U: Identification of a novel tumor necrosis factor- alpha-inducible gene, SCC-S2, con- taining the consensus sequence of a death effector domain of fas-associated death domain-like ... Engagement of the TCR by antigen-MHC results in rearrangement of the actin cytoskeleton, induction of gene transcription, and progression into the cell cycle [2,3]. The pr...

Ngày tải lên: 06/08/2014, 18:20

... distribution of character states along a cyclic ordering of the taxa, allows the identification of phylogenetically uninformative homoplastic sites in a multiple sequence alignment. Removal of these sites ... 5 of 10 (page number not for citation purposes) in the original paper [25], manual removal of homoplas- tic sites improved the trees considerably. The application of...

Ngày tải lên: 12/08/2014, 17:20

... Solution structure of the DNA binding domain of the human fork- head transcription factor AFX (FOXO4). Biochemistry 2001, 40:5861-5869. http://jbiol.com/content/2/3/20 Journal of Biology 2003, ... by the activation of any of the known cell-death pathways. Expression of the caspase inhibitors p35 or DIAP1, or of p21, an inhibitor of p53-induced apop- tosis [42], and loss...

Ngày tải lên: 06/08/2014, 18:20

... eosin using routine his- tological techniques. All of the paraffin-embedded tissue sections from normal horses were stained for lectins. Histochemical Characterization of the Lectin -binding Sites ... 2003 Histochemical Characterization of the Lectin -binding Sites in the Equine Vomeronasal Organ 17 Discussion This is the first study to examine the binding of DBA, SBA, Isole...

Ngày tải lên: 07/08/2014, 17:22

... change in the occupancy patterns of two transcription factors in the livers of ve vertebrates. © 2010 BioMed Central Ltd Dramatic changes in transcription factor binding over evolutionary time Matthew ... wealth of evidence [10] - most losses (and gains) of individual binding sites are likely to have a small effect on overall transcriptional output. In such a model,...

Ngày tải lên: 09/08/2014, 20:22

... score more in the vicinity of 10 or 11. We have found a similar pattern of behaviours at the score of 11 but not at the rec- ommended cut-off of five. Therefore, a score of five in a non-clinical ... evaluation of one of the self-report scales of DSH using Rasch analysis. Further, such evaluations of the BMC Psychiatry 2009, 9:53 http://www.biomedcentral.com/1471-244X/9/53...

Ngày tải lên: 11/08/2014, 17:20

... The success of this approach for elimination of con- taminating amplicon DNA depends on the availability of a heat-labile UNG enzyme, the heat-inactivation of which prevents cleavage of product ... availability of heat-labile UNG makes use of this approach feasible for RT-PCR. In this study, real-time RT-PCR was used to quantify UNG degradation of amplicon DNA and the effect...

Ngày tải lên: 19/06/2014, 08:20