Báo cáo y học: "Regulation of the cd38 promoter in human airway smooth muscle cells by TNF-α and dexamethasone" pot

... expression of CD38 in human air- way smooth muscle cells [5,7,8]. The regulation of CD38 expression by TNF-α in HASM cells involves NF-κB and AP-1 activation and signaling through the p38 and JNK Binding ... binding of NF-κB and AP-1 to putative cd38 binding sites. Dexamethasone reversed the TNF-α- induced activation of the 3 kb promoter an...

Ngày tải lên: 12/08/2014, 15:21

... Kayyali US, Gaestel M, Shore SA, Fredberg JJ: Role of heat shock protein 27 in cytoskeletal remodeling of the airway smooth muscle cell. Journal of Applied Physiology 2004, 96:1701-1713. 36. Dandurand ... every 10-14 days. ASM cells in culture were elongated and spindle shaped, grew with the typi- cal hill -and- valley appearance, and showed positive staining for th...

Ngày tải lên: 12/08/2014, 13:22

... carried out by comparison with a Cu 2+ standard [13]. RESULTS AND DISCUSSION Inhibition and uncoupling of the Na + -NQR activity by dioxygen In the presence of NADH and dioxygen, the purified ... already inactivated in the membrane- bound state if the cells are disrupted in the presence of dioxygen, resulting in an uncoupling of NADH oxidation and Q re...

Ngày tải lên: 31/03/2014, 21:21

... toxic to neurons in culture by increasing the vulnerability of these cells to excitotoxic and oxidative injury [50]. In smooth muscle cells, homocysteine can increase production of nitric oxide ... (b-site APP cleaving enzyme) at the N-terminus of Ab [4,5] and by an as yet unidentified c-secretase at the C-terminus of Ab (reviewedin[6]).Ab is the main compon...

Ngày tải lên: 22/02/2014, 07:20

... intense debate and remains poorly understood. The role of the airway epithelium in modifying the contractility of the underlying smooth muscle has been suggested but is not yet fully demon- strated. ... channel in the apical membrane of secretory epithelial cells [6]. In CF, defective function of CFTR in airway epithelial cells and submucosal glands...

Ngày tải lên: 12/08/2014, 16:20

... chronic inflammatory disease characterized by synovial lining hyperplasia and sublining infil- tration of inflammatory cells [1]. Fibroblast-like synoviocytes (FLSs) play a crucial role in joint ... phosphorylation by anisomycin, lipopolysaccharide, and sorbitol [19,29]. MKK4 and MKK7 are, in turn, regulated by a large family of ser- ine/threonine kinases known as the...

Ngày tải lên: 09/08/2014, 10:20

... NP cells by the action of TNF-α. Discussion This study investigates the effect of the pro-inflammatory cytokines IL-1β and TNF-α on catabolic gene expression in normal and degenerate IVD cells. ... Interleukin-1 receptor antagonist delivered directly and by gene therapy inhibits matrix degradation in the intact degenerate human interverte- bral disc: an in...

Ngày tải lên: 09/08/2014, 14:20

... possibly explained by the binding of methyl-CpG-binding protein 1 complex and methyl- CpG-binding protein 2 to methylated Sp1 transcription factor binding sites, thereby inhibiting the binding of ... role in shuttling of NF-κB from the nucleus and cytosol and in the binding NF-κB in the nucleus of T cells, potentially contributing to the lower activation lev...

Ngày tải lên: 12/08/2014, 23:22

... in the protein coding sequence [17–19]. The absence of a functional a1,3GalT gene copy in humans, and in apes and Old W orld monkeys, explains the absence of aGal epitopes in these species. In Correspondence ... METHODS Cells and cell lines COS7 cells were obtained from the Netherlands Cancer Institute (Amsterdam, the Netherlands). The pig kidney cells (P...

Ngày tải lên: 08/03/2014, 16:20

... translo- cation and in poly(U)-directed polyphenylalanine synthesis [26,27], by preventing it from binding to the ribosome [28]. Early data showed that the phosphorylation of eEF2 was increased by very low ... also increase the phosphorylation of the valyl-tRNA synthetase that associates with eEF1A/B. The available evidence suggests that phosphorylation of eEF1A/B and...

Ngày tải lên: 23/03/2014, 21:20