Báo cáo y học: "Antisense oligonucleotide inhibition of Heat Shock Protein (HSP) 47 improves bleomycin-induced pulmonary fibrosis in rats" ppt

... role of HSP47 in the progression of ble- omycin-induced pulmonary fibrosis. In fact, HSP47 pro- tein may actively participate in the pathophysiology of pulmonary fibrosis. Consequently, HSP47 antisense ... through 28 days. Hydroxyproline content in lung tissue Collagen content of bleomycin-injected lungs was assessed by the hydroxyproline assay. The concentration of hy...

Ngày tải lên: 12/08/2014, 15:20

... in our previous study also revealed a vital role for Smac in the apoptosis of myo- cytes induced by oxidative stress [12,13]. As a major stress-inducible heat shock protein, heat shock protein ... activation by eliminating inhibition of caspases by inhibitor of apoptosis protein (IAP) and is known to be a new and important regulator of apoptosis in a variety of...

Ngày tải lên: 16/03/2014, 01:20

... These inflamma- tory cytokines are key factors for activity of the Ub-pro- teasome system [36]. Thus, insulin may inhibit the activity of the Ub system by inhibiting inflammatory cytokines. However, ... degradation rates of total and myofibrillar proteins were determined by release in the medium of free tyrosine and 3-MH, respectively, and expressed as nanomoles of tyrosine/methyl...

Ngày tải lên: 11/08/2014, 06:23

... Transduction of PEP-1–HSP27 fusion protein across the blood–brain barrier. Transduction of PEP-1–HSP27 fusion protein in gerbil brain was analyzed by immunohistochemistry using antibody against histidine. ... successful delivery of full-length Tat fusion proteins by protein transduction technology. Several small regions of pro- teins, called protein transduction domains, hav...

Ngày tải lên: 18/02/2014, 17:20

... animal models of pulmonary fibrosis and possibly in patients with Idiopathic Pulmonary Fibrosis. The findings in this investigation point towards a central role of ROS in the pathophysiologic "vicious ... inflammation via induction of cytokines, chemokines and modulatory enzymes. Hyaluronan (HA), a negatively charged normally high molecular weight glycosaminoglycan, i...

Ngày tải lên: 11/08/2014, 08:22

... scored by light microscopy; some assays were stained using Giemsa. A syncytium formation value of 100% is defined as the number of syncytia formed in the absence of peptide but in the presence of 1.5% ... catalyzed by the virally encoded Env complex, which is synthesized as a polyprotein precursor and is subsequently cleaved to yield the surface glycoprotein (SU) and transmembrane...

Ngày tải lên: 13/08/2014, 05:21

... repressive effect of rapamycin is mediated through its formation of inhibitory complexes with cellular immu- nophilins, the FK506-binding proteins (FKBP). As with the cyclosporin A-cyclophilin complex, ... network, rapamycin may down-regulate a pathway linked to trans- lation of a gene which gives rise to a transcription factor with HTLV-I LTR binding capability. Alternatively rapamycin...

Ngày tải lên: 13/08/2014, 09:20

... and David Camerini* Address: Department of Molecular Biology & Biochemistry, 2230 McGaugh Hall, University of California, Irvine, Irvine, CA 92697-3900, USA Email: Ying Ye - yey@uci.edu; Jessica ... point, 3.0 × 10 6 cells were lysed in 100 µl of 100 µg/ml proteinase K in 10 mM Tris-HCl, pH 8.0 at 56°C for 1 hour, followed by heat inactivation at 95°C for 10 min. Each PCR re...

Ngày tải lên: 13/08/2014, 09:21

... clinical end points, clinical trials comparing the safety and efficacy between these DES types and BMSs have yielded inconsistent results. In the ENDEAVOR I, II,II Continued Access Registry ... outcome of ZES and PES in unselected patients treated in daily practice remains controversial. The long-term safety of DESs remains in question (10-11). Despite the results of meta...

Ngày tải lên: 25/10/2012, 11:18

... University of Thessaly), Elias Aggelopoulos, (Assistant Prof. of Psychiatry, University of Athens), Alexandros Chaidemenos (Neurologist/Psychiatrist, Director of the 8th Clinic of Psychiatric ... number of stable days and a minimal incremental cost effectiveness ratio (ICER) against risperidone (€3.39 er stable day in the case of -10% of frequency of relapses and €2.42...

Ngày tải lên: 08/08/2014, 23:21