Báo cáo y học: "Cell culture and passaging alters gene expression pattern and proliferation rate in rheumatoid arthritis synovial fibroblasts" pdf

... Changes in gene expression during passaging. (a) The changes in gene expression increase substantially during passaging of the cells in all fibroblast cultures. In addition, the variations and differences ... Neumann et al., Cell culture and passaging alters gene expression pattern and proliferation rate in rheumatoid arthritis synovial fibrob...

Ngày tải lên: 12/08/2014, 12:20

... 40:1106-1114. 42. Goronzy JJ, Weyand CM: Aging, autoimmunity and arthritis: T- cell senescence and contraction of T-cell repertoire diversity: catalysts of autoimmunity and chronic inflammation. Arthritis Res ... clustering may be explained by both genetic and environmental factors. The association between HLA-DRB1 genotypes and RA dis- ease severity, including ExRA, has been int...

Ngày tải lên: 09/08/2014, 07:20

... MMP-9, and MMP-13) are detected in RA compared with osteoarthritis synovial fluid [20]. In the RA synovium, MMP-2 is expressed in the lining and sublining layers, in addition to the synovial ... MIF-induced MMP-2 expression in rheumatoid arthritis (RA) synovial fibroblastsA schematic model of signaling pathways involved in MIF-induced MMP-2 expression in rheum...

Ngày tải lên: 09/08/2014, 08:22

... by synovial lining hyperplasia and sublining infil- tration of inflammatory cells [1]. Fibroblast-like synoviocytes (FLSs) play a crucial role in joint damage as well as the prop- agation of inflammation ... in rheumatoid arthritis synoviocytes Deepa R Hammaker 1 , David L Boyle 1 , Tomoyuki Inoue 2 and Gary S Firestein 1 1 Division of Rheumatology, Allergy and Immunology, UC...

Ngày tải lên: 09/08/2014, 10:20

... gelatin zymography, and collagenase activity assays using both SW1353 chondrosarcoma cells and primary human chondrocytes. The HDAC inhibitors trichostatin A and sodium butyrate potently inhibit ... inhibitors (TSA, phenylbu- tyrate, or FK228) block proliferation of cultured synovial fibroblasts with accompanying up-regulation of cell cycle inhibitors (p16 INK4 and p21 Cip1 ) [31,...

Ngày tải lên: 09/08/2014, 06:22

... major phenotypic shift occurs in OA toward a de-differentiated chondrocyte [40]. Interestingly, in the present study we observed increased amounts of types I and III collagens by immunohistology in both ... meniscectomy). Changes in collagen subtypes in pathological cartilage may not only influence the biomechanical integrity of the tissue but may sequester and modulate the acti...

Ngày tải lên: 09/08/2014, 06:23

... elevated in inflammatory arthritis synovial biopsies. On the contrary, IL- 1F8 protein levels were not increased in inflamed joint tissue. IL-1F8 levels measured in synovial fluids were consistently similar ... modification, and that this may alter its activity. The possible role played by IL-1F5 therefore remains unknown. Investigation by RT-PCR of their expression in joint...

Ngày tải lên: 09/08/2014, 08:22

... 100:3351-3356. 20. Lu Y, Rosenfeld R, Bar-Joseph Z: Identifying cycling genes by combining sequence homology and expression data. Bioinformatics 2006, 22: e314-322. 21. Lu Y, Mahony S, Benos PV, Rosenfeld ... Affymetrix GeneChip data in bioinformatics and computational biology solutions using R and Bioconductor. In Bioinformatics and Computational Biology Solutions Using R an...

Ngày tải lên: 09/08/2014, 22:23

... clustering reveals general activating and repressing chromatin features for individual genes As some chromatin features are positively correlated with gene expression levels and some are negatively ... histone modification may influence another by recruiting or activating chromatin-modifying complexes [27]. How- ever, a study in yeast revealed only simple and cumula- tive function...

Ngày tải lên: 09/08/2014, 22:23

... is highly effective in inhibiting HCMV gene expression and growth in human cell culture and, furthermore, demon- strate the feasibility of using DNA-based EGSs for the studies and treatment of infections ... was studied using a gel-shift assay. In this assay, the EGSs were incubated with the substrate to allow for bind- ing, and the EGS-UL49 mRNA complexes were separated i...

Ngày tải lên: 12/08/2014, 04:20