fms phospho fms and m csf in rheumatoid arthritis patients

... expression and MMP-9 activity in mice immunized for collagen-induced arthritis (a) Matrix metalloprotease (MMP)-3, MMP-9, MMP-13, ADAMTS-4 and ADAMTS-5 gene expression levels in joints following collageninduced ... CAIA and the K/BxN serum transfer models all involve immune complex formation and deposition in joints, and u-PA has been reported to be required in an immune complex-induced lung inflammation model ... report showing a requirement for u-PA in immune complex-driven lung inflammation [28] In order to explore further the proposed involvement of u-PA in immune complex-dependent inflammation we required...

Ngày tải lên: 12/08/2014, 11:23

... collagen-induced arthritis in mice – a model for rheumatoid arthritis Ageing Res Rev 2002, 1:135-147 Williams RO, Feldmann M, Maini RN: Anti-tumor necrosis factor ameliorates joint disease in murine ... structure and levels of serum interleukin-6 and tumour necrosis factor-alpha in experimental lathyrism Clin Exp Dermatol 2004, 29:168-175 Feldmann M, Maini RN: Anti-TNF alpha therapy of rheumatoid arthritis: ... B6 mice to mouse collagen, suggesting the autoimmune nature of the model It must be noted that bovine and murine collagen did not induce arthritis in B6 mice induced arthritis Sustained T-cell...

Ngày tải lên: 09/08/2014, 10:21

... timepoint, the mean loss of toluidine blue stain, mean chondron size, number of cells per mm2 and the percentage of empty chondrons was determined To determine the loss of toluidine blue staining ... and the development of collagen antibody-induced arthritis provides an informative in vivo model in which inflammatory processes can be examined in the absence of an inductive immune response ... 46 47 FcgammaRII reduces joint inflammation and destruction in experimental immune complex-mediated arthritides not only by inhibition of FcgammaRI/III but also by efficient clearance and endocytosis...

Ngày tải lên: 09/08/2014, 06:23

... 300 µg of recombinant human ALP [5,16] (kindly provided by Dr Heinzel-Wieland, Darmstadt, Germany) or 300 µg of a control protein (human serum albumin; Octapharma, Langenfeld, Germany), or received ... volume of saline only After and 24 hours, animals (n = to per time point) were anaesthetized with ketamine (90 mg/kg body weight) and xylacin (6 mg/kg) for subsequent in vivo multifluorescence microscopy ... activity was determined in accordance with the instructions of the manufacturer of the luciferase assay kit (Promega, Mannheim, Germany) with a luminometer (Luminat LB 9501; Berthold) Protein concentrations...

Ngày tải lên: 09/08/2014, 08:22

... Paraffin-embedded lung tissues were sectioned (4 m thick) and stained with hematoxylin-eosin and elastin staining (Verhoeff Van Gieson) for light microscope examination For the measurement of ... 0.1 in a closed, custom-made Styrofoam chamber Changes in RVSP were measured 5, 10, and 15 minutes after the beginning of the exposure to hypoxia Acute FiO2 of 0.1 was achieved by using a commercial ... pressure measurements, at least one hour of recovery was allowed following removal from hypoxia Animals (Wild type and TSP-1-/-) were removed from hypoxia and anesthetized with Ketamine (90 mg/kg) and...

Ngày tải lên: 10/08/2014, 09:22

... for inflammation as described in Materials and methods mPGES-1 null mice showed a reduced inflammation score compared with WT mice in response to bleomycin *P < 0.05; bleomycin-treated WT and mPGES-1 ... signifies no inflammatory cells, signifies few inflammatory cells, signifies moderate inflammatory cells, and signifies extensive inflammatory cells Alpha-smooth muscle actin immunohistochemistry Sections ... detecting macrophage infiltration in response to bleomycin treatment mPGES-1 null mice showed marked reduction in the number of macrophages (inflammation) in response to bleomycin treatment,...

Ngày tải lên: 12/08/2014, 15:22

... Immunohistochemistry using anti-α-SMA antibody was performed mPGES-1 null mice showed a reduced number of α-SMA-expressing myofibroblasts compared with WT mice in response to bleomycin treatment ... formation in vivo (a) Hydroxyproline analysis showed reduced collagen content in mPGES-1 null mice compared with wild-type (WT) mice in response to bleomycin treatment Data from four separate mice ... (PBS)-treated mice +P < 0.05; bleomycin-treated mPGES-1 null mice compared with bleomycin-treated WT mice (d) Protein extracts from skin tissue after weeks of bleomycin or PBS treatment were subjected to...

Ngày tải lên: 12/08/2014, 15:22

... immune process [3,4,33] Presumably IgG antibodies form immune complexes with CII in the cartilage matrix and initiate the complement cascade to provoke an inflammatory reaction within the joint ... collagen in patients with rheumatoid arthritis= 34) and 96), psoriatic serum of antibodies against CB10 (a) and (RA; (b) in normal human arthritis (PsA; n 33), collagen in patients with rheumatoid arthritis ... drugs including corticosteroids, non-steroidal anti-inflammatory drugs, or diseasemodifying anti-rheumatic drugs, including methotrexate Sera were also obtained from 33 patients with PsA and from...

Ngày tải lên: 09/08/2014, 01:23

... compartment model of immune-mediated disease with implications for immunotherapeutic strategies Clin Immunol Immunopathol 1994, 73:27-37 Rosloniec EF, Brand DD, Myers LK, Whittington KB, Gumanovskaya ... time points after immunization is common to both DR1 and DR4 transgenic mice In addition to A12, we identified another altered peptide ligand, A13, containing substitutions within the immunodominant ... Brand DD, Myers LK, Esaki Y, Whittington KB, Zaller DM, Woods A, Stuart JM, Kang AH: Induction of autoimmune arthritis in HLA-DR4 (DRB1*0401) transgenic mice by immunization with human and bovine...

Ngày tải lên: 09/08/2014, 08:22

... autoimmunity in patients with RA CII autoimmunity may be a secondary phenomenon induced following inflammation in the joints and may play a role in the persistence of the disease rather than in ... double-blind, double-dummy, randomized, and MTX-controlled trial comparing efficacy and safety of CCII and MTX in the treatment of RA Patients were randomly assigned to a CCII (n = 326) or MTX (n ... tropicalis/ovalbumin murine co-immunization model J Clin Immunol 2005, 25:153-161 Staines NA, Derry CJ, Marinova-Mutafchieva L, Ali N, Davies DH, Murphy JJ: Constraints on the efficacy of mucosal tolerance in...

Ngày tải lên: 12/08/2014, 11:22

... 10 minutes at room temperature and permeabilized in 0.1% Triton X-100 in PBS for 15 minutes at 4°C After washing (10 mM TRIS HCl pH 7.6, containing 150 mM NaCl, TBS buffer) and blocking (normal ... Culture media ingredients and inhibitors were purchased from Sigma Chemical Co (Dorset, UK), unless stated otherwise Cells were grown in culture medium (RPMI 1640 medium containing mM L-glutamine, ... hours in the absence or presence of inhibitors of uptake (10.0 M cytochalasin D, 5.0 M monodansylcadaverine, 1.0 mM amiloride, 0.2 mM 5-(N,Ndimethyl) amiloride (DMA) or 0.4 μg/ml filipin) for...

Ngày tải lên: 09/08/2014, 08:22

... containing 10% FCS, 10 M b-mercaptoethanol, 10 mM HEPES, penicillin, and streptomycin (complete media, cDMEM) in humidified incubator at 37C in 7.5% CO2 Cells were stimulated with 25 g/ml of lathyritic ... were involved in performing the majority of experiments and drafted the manuscript RM was involved in assisting and planning the CIA experiments and helped to draft the manuscript, KSN was involved ... wished to investigate cystatin C involvement in an in vivo autoimmune process in a well-defined animal model Collagen induced arthritis (CIA) has been extensively used as an animal model for human...

Ngày tải lên: 12/08/2014, 15:22

... multivitamin and mineral supplements containing vitamins and minerals as the sole medicinal ingredients Use of concomitant prohibited medication (narcotics, oral NSAIDs, topical NSAIDs) within ... Visit Randomization Clinical assessments as indicated; first dose in clinic Clinical assessments as indicated Clinical assessments as indicated Clinical assessments as indicated 30 60 Treatment ... and safety of undenatured type II collagen singly or in combination with glucosamine and chondroitin in arthritic dogs Tox Mech Methods 2007; 17:189-196 Faria AM, Weiner HL Oral tolerance Immunol...

Ngày tải lên: 26/10/2012, 09:48

... in nontolerized CIA mice and normal DBA/1 mice in all three organs examined (Table 2) In normal DBA/1 mice the proportion of IL-10-producing CD4+CD25+ cells remained unchanged from baseline in ... dissolved in 0.05 N acetic acid at mg/ml and emulsified with an equal volume of complete Freund’s adjuvant (CFA) As primary immunization, 0.1 ml of the emulsion, containing 100 µg CII, was injected into ... involved in the induction of peripheral tolerance in an orally tolerized mouse model of arthritis R218 Our findings indicate that the serum levels of proinflammatory and anti-inflammatory IgG subtypes...

Ngày tải lên: 09/08/2014, 01:23

... levels of col II in osteoarthritic joints were fourfold those in normal joints in the lateral compartment (P < 0.001) and were sevenfold those in normal joints in the medial compartment (P

Ngày tải lên: 09/08/2014, 06:22

... the hybridomas were expanded in DMEM Glutamax-1 medium containing 0.5% streptomycin and 0.6% penicillin and Ultra low IgG fetal bovine serum (FBS) (Gibco BRL, Grand Island, NY, USA) The mAbs were ... inflammatory cell types in the development of erosive arthritis Arthritis Rheum 1987, 30:460-465 Nandakumar KS, Andren M, Martinsson P, Bajtner E, Hellstrom S, Holmdahl R, Kleinau S: Induction of arthritis ... glycosylated in normal cartilage but modified by arthritis in both rats and humans Eur J Immunol 2005, 35:357-366 Daumer KM, Khan AU, Steinbeck MJ: Chlorination of pyridinium compounds Possible...

Ngày tải lên: 09/08/2014, 07:20

... consisted of RPMI-1640, mM l-Glutamine, 1% non-essential amino acids, 1% sodium pyruvate, 100 U/ml penicillin, 100 µg/ml streptomycin, 25 mM HEPES Buffer, and 55 M 2-mercaptoethanol (all from Invitrogen, ... alloactivated T lymphocytes in systemic sclerosis and Raynaud's phenomenon Clin Exp Immunol 1999, 116:164-168 Duncan M R, Berman B: Gamma interferon is the lymphokine and beta interferon the monokine responsible ... PBMCs were resuspended at × 107 cells per ml in phosphate-buffered saline (PBS) containing 0.1% bovine serum albumin (BSA) (Sigma-Aldrich) Prior to staining, an aliquot of CFSE (5 mM in dimethyl...

Ngày tải lên: 09/08/2014, 08:22

... Glomerular infiltration of monocytes/macrophages is frequently observed in a variety of human LN [30,31] and the chemokine most commonly involved in renal monocytes/macrophages recruitment is MCP-1 ... concentration of dexamethasone inhibited the LPS-induced increase in MCP-1 mRNA (Figure 5a) and in OPN mRNA (Figure 5b) levels in NZB/W MCs and the LPS-induced increase in protein levels of MCP-1 (ELISA; ... collagenase, and the dissociated glomerular cells were cultured in RPMI 1640 medium containing 20% heat-inactivated endotoxin-free FBS, penicillin/streptomycin, and HEPES (10 mM) (GIBCO, Invitrogen,...

Ngày tải lên: 09/08/2014, 10:20

... citrullinated antigens Mol Immunol 2008, 45:2808-2819 Okroj M, Heinegård D, Holmdahl R, Blom AM: Rheumatoid arthritis and the complement system Annals Med 2007, 39:517-530 Song H, Qiao F, Atkinson ... paraffin The front ankle joints were sectioned at m and stained with hematoxylin and eosin (H&E) by the standard technique For immunohistochemical staining, the sections were deparaffinized and ... Excluding CM-5 from the cocktail induced a more severe form of arthritis Furthermore, experiments in vitro showed that the activation of complement and binding to mouse CII of CM-5 were much less...

Ngày tải lên: 11/08/2014, 03:20

... citrullinated antigens Mol Immunol 2008, 45:2808-2819 Okroj M, Heinegård D, Holmdahl R, Blom AM: Rheumatoid arthritis and the complement system Annals Med 2007, 39:517-530 Song H, Qiao F, Atkinson ... paraffin The front ankle joints were sectioned at m and stained with hematoxylin and eosin (H&E) by the standard technique For immunohistochemical staining, the sections were deparaffinized and ... Excluding CM-5 from the cocktail induced a more severe form of arthritis Furthermore, experiments in vitro showed that the activation of complement and binding to mouse CII of CM-5 were much less...

Ngày tải lên: 11/08/2014, 06:23