báo cáo khoa học: " Bioinformatic analysis of the CLE signaling peptide family" ppt

... CLE sequences from various plant species CLE3 4, CLE3 6, CLE6 4, CLE7 8, CLE8 0, CLE1 17, CLE1 18 and CLE1 63, due to the redundancy in the conserved domain. Because the CLE domain that was used for clustering ... of the Multidomain CLE sequencesFigure 1 Multidomain CLE sequences. The potential multidomain CLE signaling peptides CLE7 5, CLE7 6, CLE6 8, CLE3...

Ngày tải lên: 12/08/2014, 05:20

... related to the reduction of the PQ pool. Results As reported in the literature, the I step of the O–J–I–P fluorescence transient cannot be clearly distinguished by visual analysis of the FI traces ... amplitudes and rate constants of the two other phases. The above should be gov- erned by the balance between the rate of reduction and oxidation of the accepto...

Ngày tải lên: 19/02/2014, 05:20

... connected to the DEC1 E-boxes (Fig. 2), while deletion of the Orange domain ( DEC1DOr- ange) had no effect on the suppressive activity, indicating the importance of t he bHLH region of DEC1. The HXXXXXXXR ... conclude that the basic region, p artic- ularly the conserved A rg65, but not His57, is essential for the suppressive activity of DEC1. Determination of the b...

Ngày tải lên: 19/02/2014, 16:20

... Addition of the L2 peptide (dansyl-GSKL) was safely possible due to the conserved binding site and the presence of a YQSKL pentapeptide in the modelling template. Modelling of the longer peptide ... investigate the binding of the dansylated PTS1 peptide L2 to HpPex5p and correlate the fluorescence data with structural data, a molecular model of the HpPex5p wa...

Ngày tải lên: 21/02/2014, 00:20

... of the structure of NADP + -bound FeR revealed that the nicotinamide moiety of NADP + faces the re-side of the isoalloxazine ring, and that the 2¢-P-AMP moiety is held in the groove between the ... direction along the b10 of the other mono- mer, forming an anti-parallel intermolecular b sheet (Fig. 2A). The subsequent residues of the loop (Ala7– Pro13) turn i...

Ngày tải lên: 07/03/2014, 02:20

... proteins. The 3D arrangement of the binding sites for TIPRL and a4 on the surface of PP2Ac shows that they are in close proximity, but not overlapping, which allows the assembly of the TIPRL:PP2Ac:a4 ... indicates the elution positions of molecular mass markers are shown above the panels. The profiles of the three proteins overlap over a region that coincides with...

Ngày tải lên: 07/03/2014, 05:20

... incomplete process- ing of the signal peptide was also reported [16,17]. The uncleaved part of the a-mating sequence at the N-ter- minus as well as the histidine tag at the C-terminus of the recombinant ... lines and the curved blue arrows indicate the overall orientation of the cavities. (C), (F), (I) Ribbon diagrams at the top of the Man-binding lectins s...

Ngày tải lên: 14/03/2014, 23:20

... introducing features of the consensus motif into the Zm-p60.1 scaffold, an analysis of structural and catalytic prop- erties of the mutants, and simulations of the sub- strate–enzyme interactions of the wild-type ... F200K and P2. Furthermore, the F193A and W373K muta- tions do not result in any change in the thermostability of the enzyme (Table S2), and thermal...

Ngày tải lên: 16/03/2014, 04:20

... A milestone in the study of peptide s ynthetases was the determination of the A-domain structure of GrsA Phe -A [8]. Aspects of speci®city and binding were superimposed on other A-domains and with the help ... selectivity of the A-domains [9] simultaneously determines the primary sequence of the product. Subsequently the a ctivated amino acid i s tethered to the...

Ngày tải lên: 17/03/2014, 11:20

... in the NdeI site of the pET15b vector (Novagen, Darmstadt, Germany). In this construct, the His6-tag sequence was fused to the N-terminus of the protein. The EVL fragments were expressed in the ... described previously [28,29]. The con- centrations of the RAD51 and EVL proteins were determined with the Bradford method, using BSA as the standard. The concentration...

Ngày tải lên: 23/03/2014, 04:20