báo cáo khoa học: " Analysis of cDNA libraries from developing seeds of guar (Cyamopsis tetragonoloba (L ) Taub)" ppsx

... Central Page 1 of 12 (page number not for citation purposes) BMC Plant Biology Open Access Research article Analysis of cDNA libraries from developing seeds of guar (Cyamopsis tetragonoloba (L. ) Taub) Marina ... the development of guar seeds. Results and Discussion Generation of cDNA libraries Figure 1 shows sections of developing guar seed...

Ngày tải lên: 12/08/2014, 05:20

... initial step of the isolation of AIVAS from VAAS, 6 mL of VAAS (3.6 mgÆmL )1 total protein) was incubated with 3 mL of LL–Sepharose and the bound proteins were eluted with 6 mL of the elution ... (2000 kDa); BSA (65 kDa); egg albumin (45 kDa); chymotrypsinogen (25 kDa); cytochrome c (12.3 kDa); aprotinin (6.5 kDa); and l-tyrosine (0.1 kDa). Proteins were eluted at 0.5 mLÆ min...

Ngày tải lên: 23/03/2014, 07:20

... Western blotting. Core histones from chicken erythrocytes (lanes c) and from pea (lanes p) were separated by SDS/PAGE. (A) shows a Coomassie- stained gel and (B) a similar gel blotted and probed ... minutes) and sedimented. Proteins recovered from the supernatant (lanes S) after 25% (w/v) trichloroacetic acid precipitation and the acid-soluble proteins from the residual nuclear pellet...

Ngày tải lên: 08/03/2014, 10:20

... spectrum. (D) Single-scan, ETD ⁄ PTR tandem mass spectrum of [M + 11H] +11 ions (m ⁄ z 492. 9) from a protein that elutes at 34 min [peak II in (A)], $ 3 min after peak I. Spectra in (C) and (D) contain ... function of biologically important proteins. Rev (regulator of expression of virion prod- ucts) is one such protein. Rev is expressed by HIV-1 and mediates the export of uns...

Ngày tải lên: 18/02/2014, 16:20

... suggesting the existence of some interactive cellular partner. FRAP analysis of the nuage revealed the slow recovery of Olvas–GFP (Fig. 3B). When the whole part of the single compartment of nuage was bleached, ... min B A Fig. 2. Time-lapse LSM analysis of Olvas–GFP reveals the dynamic nature of the nuage. Schematic diagram of the preparation of PGCs of medaka specime...

Ngày tải lên: 18/02/2014, 16:20

... 100 0) (Molecular Probes ⁄ Inter- chim, Montluc¸ on, France), anti-Hdj-1(Hsp4 0) (1 : 100 0) (Stressgen, Victoria, Canada), anti-Hsp27 (1 : 20 0) (Stress- gen), anti-Hsp70 (1 : 100 0) (Stressgen) or ... procedures. (B) Confocal immunofluorescence analysis of transfected cell population. COS-7 cells were transfected with vectors encoding either (a) httEx1-25Q-EGFP, (b) httEx1...

Ngày tải lên: 19/02/2014, 06:20

... extension of our joint inference approach we may soon be able to exceed the performance of a single manual annotator. Our analysis of the types of errors, and the per- formance of our knowledge ... identification (0.6% vs. 0.4 %) and classification (0.8% vs. 0.4 %) over single- document coreference. 7 Discussion The use of 'feedback' from subsequent sta...

Ngày tải lên: 20/02/2014, 12:20

... itself if it is of type REL). After the analysis of "Bob mat", the situation of the parse tree would be as in fig.2.a (and p~l is the current node). Tne analysis of "Sue" ... (usually a dialect of PROLOG) has to keep track of the binding of the variables, of the clauses that have not been used (but could be used in case of failure of the curre...

Ngày tải lên: 21/02/2014, 20:20

... R68A )) ) )) ) 5E R67A ⁄ R68A ⁄ K72A )) ) )) ) )) ) )) 5A,D K159A ⁄ K160A )) ) )) ) )) ) )) 5A,D a 25 mM Mes (pH 6. 5), 1 mM MgCl 2 , and 1 mM dithiothreitol. b Protein aggregated during the process of ... stronger than that of the wild type; ), DNA-binding activity is slightly weaker than that of the wild type; )) ), DNA-binding ac...

Ngày tải lên: 06/03/2014, 09:22

... Strain MPS26 was trans- formed with pGFP–N-LSM1 (Lsm 1), pMR144 (1N), pMR133 (1C), pGFP–N-LSM8 (Lsm 8), pMR132 (8N), pMR156 (8C) or pGFP–N-FUS (GFP; Table S 1). Cells were grown in SD–Ura–Met and localization ... of HA–Lsm8p after 10 h of growth on glucose (Fig. 9C). Northern analysis of U6 (Fig. 9A) and U4 snRNAs (Fig. 9B) after nondenaturing PAGE showed decreased AB C Fig. 9....

Ngày tải lên: 07/03/2014, 02:20