báo cáo khoa học: " Subcellular localisation of Medicago truncatula 9/13-hydroperoxide lyase reveals a new localisation pattern and activation mechanism for CYP74C enzymes" doc
báo cáo khoa học: " Transcriptional profiling of Medicago truncatula under salt stress identified a novel CBF transcription factor MtCBF4 that plays an important role in abiotic stress responses" ppt
... underlined) and 5’-AT A CTA GTT TAA AAT GAG TAA CTC CAC A- 3’ (Spe I site underlined). An NcoI-SpeI fragment containing MtCBF4 cDNA was inserted into pCAMBIA1302 con- taining the 35S CaMV promoter and a ... GGA TGG TAG ATC TGA CTA GT-3’ (XmaI site underlined) and 5’-AT TCT AGA TT A GTG GCT AGC T’TT GTA TAG-3’ (XbaI site underlined). This created a new vector named pE3025-GFP....
Ngày tải lên: 11/08/2014, 11:21
báo cáo khoa học: " Transcriptional profiling of Medicago truncatula meristematic root cells" docx
... truncatula and provides candidates for functional analysis. Background The root and shoot apical meristems (RAM and SAM) are established during embryogenesis and serve as a source of stem cells for ... Spectrophotometer; RNA with an absorbance A 260 /A 280 ratio > 2.0 was quality tested using the Agilent 2100 Bioanalyzer. Preparation of cRNA, hybridization, and sc...
Ngày tải lên: 12/08/2014, 05:20
Tài liệu Báo cáo khoa học: Identification of Ewing’s sarcoma protein as a G-quadruplex DNA- and RNA-binding protein ppt
... ACA G) and EWS reverse d(CGC TCG AGT CAC TAG TAG GGC CGA TCT CTG C), for pGEX–EWS; EAD forward d(CGG AAT TCA TGG CGT CCA CGG ATT ACA G) and EAD reverse d(CGC TCG AGT CAT CCG GAA AAT CCT CCA GAC ... T), for pGEX–EAD; RGG1 forward d(CGG AAT TCC CAG GAG AGA ACC GGA GCA T) and RGG1 reverse d(CGC TCG AGT CAA TCA AGA TCT GGT CCT TCA TCC ATG G), for pGEX–RGG1; RRM– RGG2–ZnF forward d(CGG...
Ngày tải lên: 15/02/2014, 01:20
Báo cáo khoa học: Subcellular compartmentalization of FADD as a new level of regulation in death receptor signaling pdf
... differential pathways directing FADD nuclear export and cytoplasmic trafficking, and identify subcellular compartmentalization of FADD as a novel regulatory mechanism in death receptor signaling. Abbreviations BFA, ... and the cytoplasm. Whereas cytoplasmic TRADD mediates apoptosis through FADD and caspase-8 activation, nuclear TRADD acts through a mitochondrial apoptosis pa...
Ngày tải lên: 07/03/2014, 02:20
Báo cáo khoa học: Subcellular localization of yeast Sec14 homologues and their involvement in regulation of phospholipid turnover pptx
... Name Primer 5¢ upstream of SFH1 SFH1/1D 5¢-ATGAAAAGAGGTCGGCATAG-3¢ SFH1/1R 5¢- AGCTAAACAGATCTGGCGCGCCTTAAGTATGCTGGTTGTCATTCTTCC-3¢ Downstream of SFH1 SFH1/2D 5¢- GTCGAAAACGAGCTCGAATTCATCGAGATACATCGGACCAGAAGGTG-3¢ SFH1/2R ... upstream of SFH2 SFH2/1D 5¢-ACGAGGCGGTCTCTGTTCTCTG-3¢ SFH2/1R 5¢- AAGCTAAACAGATCTGGCGCGCCTTATGCTTTTATGCTTCTGTGTGCG-3¢ Downstream of SFH2: SFH2/2D 5¢- GTCGAAAACGAG...
Ngày tải lên: 23/03/2014, 18:20
Tài liệu Báo cáo khoa học: Gene transcription of fgl2 in endothelial cells is controlled by Ets-1 and Oct-1 and requires the presence of both Sp1 and Sp3 pdf
... 5¢-TGT CGAATGCAAATCACTAGAA-3¢; Sp1, 5¢-ATTCGATC GGGGCGGGGCGAGC-3¢; Ets/Pea3, 5¢-GATCTCGAG CAGGAAGTTCGA-3¢; Ets (PU.1), 5¢-GGGCTGCTTG AGGAAGTATAAGAAT-3¢;Stat3,5¢-GATCCTTCTG GGAATTCCTAGATC-3¢; and C/EBP, ... TCT GAG GTA CCA ATG CAA ATG CGC Mu-AS)97/)91 GCG CAT TTG CAT TGG TAC CTC AGA CGC CAC Mu-S)87/)80 GAG ACT GTG ATG CGG TAC CTC CCG CCC TTT C Mu-AS)87/)80 GAA AAG GGC GGG AGG TAC CGC ATC ACA...
Ngày tải lên: 20/02/2014, 11:20
Báo cáo khoa học: Fluorescence studies of the replication initiator protein RepA in complex with operator and iteron sequences and free in solution pdf
... GAACAAGGACAGGGCATTGACTTGTCCCTGTCCCTTAAT 1DR 45 ATACCC GGGTTTAAAGGGGACAGATTCAGGCTGTTATCCACACCC 1DR-short 30 GCCC GGGTTTAAAGGGGACAGATTCAGGCC A D B E C F Fig. 3. Excitation spectra (k em = 480 nm) of AEDANS C160–RepA with ... convoluted experimental data and resulting fits. In the case of AEDANS, individual analysis of 480 and 530 nm decays gave very similar lifetime values. Theref...
Ngày tải lên: 07/03/2014, 04:20
Báo cáo khoa học: Nuclear localization of human spermine oxidase isoforms – possible implications in drug response and disease etiology pptx
... primer pair 5¢-CAATC CTC GAGTATGCAAAGTTGTGAATCCAG-3¢ and 5¢-TAA T AAGCTTTGGTCCCCTGCTGGAAGAGGTC-3¢ and each cDNA in pET15b as the template. PCR products were restriction digested with XhoI and HindIII ... human non-small cell lung carcinoma, A5 49 human lung adenocarcinoma and Beas2B transformed, non-tumorigenic, human lung epithelium (ATCC, Manassas, VA, USA). H157 and A5 49 cells...
Ngày tải lên: 07/03/2014, 05:20
Báo cáo khoa học: Modulatory effects of plant phenols on human multidrug-resistance proteins 1, 4 and 5 (ABCC1, 4 and 5) potx
... ATGGCCAAAATCACAAGGGTTAGC ABCC1 1119–1670 AGTGGAACCCCTCTCTGTTTAAG CCTGATACGTCTTGGTCTTCATC ABCC4 3880–4124 TGATGAGCCGTATGTTTTGC CTTCGGAACGGACTTGACAT ABCC5 3692–3864 AGAGGTGACCTTTGAGAACGCA CTCCAGATAACTCCACCAGACGG ABCC11 ... for the ABC transporters for quantitative real-time RT-PCR. ABC transporter Position of primer Forward oligo sequence Reverse oligo sequence ABCB1 834–1086 GCCTGGCAGCTG...
Ngày tải lên: 07/03/2014, 21:20
Báo cáo khoa học: Sequences downstream of the transcription initiation site are important for proper initiation and regulation of mouse ribonucleotide reductase R2 gene transcription ppt
... and Py-Im targets CÆGbasepairs and the Py-b-alanine pair recognizes both A TandT A base pairs [38]. The boxed sequence represents the atypical R2 TATA-box. Black and white circles, Im and Py rings, ... that contain the native R2 TATA- box and either the natural or the deleted R2 5¢-UTR (TATA Wt UTR Wt and TATA Wt UTR mut , respectively). We found that addition of increasing am...
Ngày tải lên: 08/03/2014, 02:20