báo cáo khoa học: " Tagging of MADS domain proteins for chromatin immunoprecipitation" pdf

... expression. pARC113 A pARC258 (pK7WGF2) pARC259 (pK7FWG2) pARC064 (Alligator2) gene gene gene gene tNOS tNOS t35S t35S B C D eGFP eGFP 3xHA StrepII-FLAG p35S p35S p35S p35S 0.5 kb BioMed Central Page 1 of 11 (page number not for citation purposes) BMC Plant Biology Open Access Research article Tagging of MADS domain proteins for chromatin immunoprecipitation Stefan ... gene...

Ngày tải lên: 12/08/2014, 05:20

... K m values of the mutant constructs may be caused predominantly by an increased off-rate for dissociation of the Michaelis complex. For the time being, we are limited to speculation because off-rates have ... catalyze the formation of 2-amino-5-formyl- amino-6-ribofuranosylamino-4(3H)-pyrimidinone triphosphate. J Biol Chem 274, 16727–16735. 17 Nar H (2004) GTP cyclohydrolase I. In H...

Ngày tải lên: 18/02/2014, 11:20

... have been strongly recommended for most analyses that require a large quantity of transcripts, proteins or cells. For immortal- ization of ECs, viral oncogenic proteins have been used in previous ... Japan) for providing pSA-bgeo; Dr Izumo Saito (Institute of Medical Science, University of Tokyo, Japan) for providing pCAG-Cre; Dr Ikuo Yana (Institute of Medical Science,...

Ngày tải lên: 18/02/2014, 17:20

... expression data of Cg-Clp1 and Cg-Clp2 transcripts. The relative level of expression of each target gene was calculated for 100 copies of GAPDH transcript by using the following formula: N ¼ 100 ... with a Glyco_18 domain (in a catalytically inactive form [5]) followed by an addi- tional C-terminal sequence of about 90 amino acids of unknown function. The high degree of iden...

Ngày tải lên: 19/02/2014, 00:20

... select or screen proteins with the desired properties from libraries of mutant proteins have been sought [5–8]. Phage-display coupled with proteolysis for selection of stably folded proteins was ... spectra indicated formation of a b-sheet structure consistent with the segment from the CspA. Thermal melting of the selected proteins was cooperative. The thermodynamic stabilit...

Ngày tải lên: 19/02/2014, 12:20

... as a whole, tagging sections of text by function (e.g., abstract, introduction, etc.), tagging sen- tences/clauses, and tagging participants in clauses. To overcome the complexity of dealing ... embedding of segments. Annotated texts are stored using stand-off XML, one file per source text and layer. See Figure 4 for a sample. The software does not currently input from or...

Ngày tải lên: 20/02/2014, 09:20

... always an easy task and often requires the use of a morphological analyzer. One common example in Arabic nouns is Broken Plurals. For example, one of the plu- ral forms of the Arabic word kAtb ... Ambiguity: The form of cer- tain letters in Arabic script allows suboptimal or- thographic variants of the same word to coexist in the same text. For example, variants of Hamzated Al...

Ngày tải lên: 20/02/2014, 11:21

... used to make the buffers of specific pH value are as follows; Mes for pH 6.0, Hepes for pH 7.0, Tris for pH 8.0 and 9.0, TAPS for pH 10.0. The reaction was incu- bated for 24 h with I-CsmI(237) ... 80–30% of the wild-type substrate for I-SpomI and I-CsmI, while 60–42% for I-SceII; for I-ScaI, efficiency of originally described as ‘reduced cleavage’. Not or scarcely cleaved...

Ngày tải lên: 07/03/2014, 17:20

... residues contribute to the processing of the p100 and p105 NF-jB precursor proteins for p50 and p49/p52. A prerequisite for the proteolytic cleavage of p105 is the phosphorylation of serines 894 and 908, which ... therefore exert its regulatory role before or after translocation of p65 and p50 to the nucleus. Beside the well-studied induction of CREB, there has been little evi...

Ngày tải lên: 08/03/2014, 02:20

... fields. It is com- mon for users to specify location information in the SearchTerm field. For example, “restaurants near Manhattan” for SearchTerm and “NY NY” for LocationTerm. For voice-based search, ... of the test set where the string identified by the parser for a slot is ex- actly the same as the annotated string for that slot. Table 3 reports parsing performance using the P...

Ngày tải lên: 08/03/2014, 21:20